Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,240	A→G	I280T (ATC→ACC)	mdtO ←	membrane translocase (MDR) of MdtNOP efflux pump, PET family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,240	0	A	G	100.0%	127.8 / NA	36	I280T (ATC→ACC)	mdtO	membrane translocase (MDR) of MdtNOP efflux pump, PET family
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/20); total (16/20)

CGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGT  >  NC_000913/4302125‑4302373

cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                                <  2:672176/139‑1 (MQ=255)

cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                                >  2:280824/1‑139 (MQ=255)

 gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTTACTGATCCGCCAGTCGCTTTGCCAGCTCTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGATTCTGAAGCTAATTTTTGTCGGa                                                                                                               >  1:622291/1‑139 (MQ=255)

 gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGa                                                                                                               >  1:339649/1‑139 (MQ=255)

  tCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGaa                                                                                                              <  1:424144/139‑1 (MQ=255)

                    ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                            >  1:531397/1‑139 (MQ=255)

                    ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                            <  2:225630/139‑1 (MQ=255)

                     gTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGaa                                                                                           >  2:636808/1‑139 (MQ=255)

                      ttACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAAt                                                                                          <  2:622291/139‑1 (MQ=255)

                             cccGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCaaa                                                                                   <  2:339649/139‑1 (MQ=255)

                               cGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                                 <  1:443302/107‑1 (MQ=255)

                               cGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                                 >  2:443302/1‑107 (MQ=255)

                                gTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAaga                                                                                <  2:639170/139‑1 (MQ=255)

                                 tGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAagag                                                                               >  1:358806/1‑139 (MQ=255)

                                   ccgccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGt                                                                             >  1:780662/1‑139 (MQ=255)

                                      ccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTggg                                                                          <  1:776402/139‑1 (MQ=255)

                                             ttcactttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAg                                                                   <  1:158509/139‑1 (MQ=255)

                                                  tttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGAtt                                                              <  1:21806/139‑1 (MQ=255)

                                                        tGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                                        <  1:712852/139‑1 (MQ=255)

                                                        tGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                                        <  2:358806/139‑1 (MQ=255)

                                                         gATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTc                                                       >  2:218803/1‑139 (MQ=255)

                                                          aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                                 >  1:500408/1‑80 (MQ=255)

                                                          aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                                 <  2:500408/80‑1 (MQ=255)

                                                          aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCg                                                      >  1:435654/1‑139 (MQ=255)

                                                           tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                                     >  1:219208/1‑139 (MQ=255)

                                                             cGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                   <  1:636808/139‑1 (MQ=255)

                                                             cGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                   <  2:583172/139‑1 (MQ=255)

                                                             cGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                   <  1:280824/139‑1 (MQ=255)

                                                                    cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTa                                            >  2:92592/1‑139 (MQ=255)

                                                                                   gtccTTCAGCAACTGCATGCTGCTGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATACTTGCCTGACAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCAc                             <  1:622833/137‑1 (MQ=255)

                                                                                   gACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCAc                             <  2:435654/139‑1 (MQ=255)

                                                                                    aCCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                         <  1:503721/110‑1 (MQ=255)

                                                                                    aCCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                         >  2:503721/1‑110 (MQ=255)

                                                                                               cGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTTCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCTTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGcca                 >  1:75010/1‑139 (MQ=255)

                                                                                                           gCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTg     <  1:369825/139‑1 (MQ=255)

                                                                                                              tgttgGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGt  >  2:263833/1‑139 (MQ=255)

CGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGT  >  NC_000913/4302125‑4302373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A