Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,240	A→G	I280T (ATC→ACC)	mdtO ←	membrane translocase (MDR) of MdtNOP efflux pump, PET family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,240	0	A	G	100.0%	175.9 / NA	49	I280T (ATC→ACC)	mdtO	membrane translocase (MDR) of MdtNOP efflux pump, PET family
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/25); total (24/25)

CCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCA  >  NC_000913/4302111‑4302361

cccAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAg                                                                                                                  <  1:470509/139‑1 (MQ=255)

             acGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     >  1:1109464/1‑139 (MQ=255)

              cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                    >  2:605932/1‑139 (MQ=255)

               gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGa                                                                                                   >  1:1112950/1‑139 (MQ=255)

                             ctcCAGGTTACATTCCCGTGTCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTAAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGcc                                                                                     <  2:229419/139‑1 (MQ=255)

                             ctcCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGcc                                                                                     >  1:160185/1‑139 (MQ=255)

                               ccAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTg                                                                                   >  2:807437/1‑139 (MQ=255)

                                  ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                <  1:487418/139‑1 (MQ=255)

                                  ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                >  2:231901/1‑139 (MQ=255)

                                     tACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATc                                                                             >  1:404775/1‑139 (MQ=255)

                                      aCATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCa                                                                            <  2:404775/139‑1 (MQ=255)

                                              gTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAaga                                                                    >  1:1076098/1‑139 (MQ=255)

                                                  cgccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTg                                                                >  2:455110/1‑139 (MQ=255)

                                                              actttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGa                                                    >  1:184508/1‑139 (MQ=255)

                                                                 ttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     >  1:176845/1‑87 (MQ=255)

                                                                 ttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     <  2:176845/87‑1 (MQ=255)

                                                                       gATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCaaaa                                                                      <  1:76926/112‑1 (MQ=255)

                                                                       gATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCaaaa                                                                      >  2:76926/1‑112 (MQ=255)

                                                                         tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                         <  2:1112950/139‑1 (MQ=255)

                                                                         tCCGCAAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGACTGAGAAt                                                                              >  1:145311/1‑102 (MQ=255)

                                                                         tCCGCAAGTCGCTTTGCCAGCACTGAACTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTAGTCGGAATTCAATAATTGCCTGAGAAt                                                                              <  2:145311/102‑1 (MQ=255)

                                                                          ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                            >  1:628014/1‑135 (MQ=255)

                                                                          ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                            <  2:628014/135‑1 (MQ=255)

                                                                          ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                        <  1:455110/139‑1 (MQ=255)

                                                                           cGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                       <  2:131947/139‑1 (MQ=255)

                                                                             ccAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCCGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaataa                                                                                          >  1:743994/1‑86 (MQ=255)

                                                                             ccAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaataa                                                                                          <  2:743994/86‑1 (MQ=255)

                                                                               aGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAAt                                   <  2:1076098/139‑1 (MQ=255)

                                                                                gTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGaa                                                                                                                   <  2:1112964/58‑1 (MQ=255)

                                                                                gTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGaa                                                                                                                   >  1:1112964/1‑58 (MQ=255)

                                                                                  cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     <  1:238283/70‑1 (MQ=255)

                                                                                  cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     >  2:238283/1‑70 (MQ=255)

                                                                                   gCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAg                               <  2:160185/139‑1 (MQ=255)

                                                                                                  aCCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGACGGAATTCAATAATTGCCTGAGAAGCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACg                >  1:681026/1‑139 (MQ=255)

                                                                                                   ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                       >  1:257628/1‑51 (MQ=255)

                                                                                                   ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                       <  2:257628/51‑1 (MQ=255)

                                                                                                   ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGc               <  2:1109464/139‑1 (MQ=255)

                                                                                                     ttCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAg             <  2:185879/139‑1 (MQ=255)

                                                                                                       cAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                    <  2:383784/50‑1 (MQ=255)

                                                                                                       cAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                    >  1:383784/1‑50 (MQ=255)

                                                                                                               gCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                       >  2:577678/1‑39 (MQ=255)

                                                                                                               gCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                       <  1:577678/39‑1 (MQ=255)

                                                                                                                cATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGCATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGccacca  <  2:681026/139‑1 (MQ=255)

                                                                                                                       cAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                         <  1:862765/93‑1 (MQ=255)

                                                                                                                       cAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                         >  2:862765/1‑93 (MQ=255)

                                                                                                                        aGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAAt                                   <  2:295785/98‑1 (MQ=255)

                                                                                                                        aGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAAt                                   >  1:295785/1‑98 (MQ=255)

                                                                                                                             gttgGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAg                                               >  2:143785/1‑81 (MQ=255)

                                                                                                                             gttgGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAg                                               <  1:143785/81‑1 (MQ=255)

CCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCA  >  NC_000913/4302111‑4302361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A