Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	551,332	G→A	298 (TAG→TAA)	ybcF →	putative carbonate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	551,332	0	G	A	100.0%	150.0 / NA	47	298 (TAG→TAA)	ybcF	putative carbonate kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/23); total (24/23)

GATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGT  >  NC_000913/551198‑551467

gATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                     <  1:791726/139‑1 (MQ=255)

   ggTTCGATGGGGCCGAATGTAACGGTGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAgg                                                                                                                                  <  2:286043/139‑1 (MQ=255)

        gATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTa                                                                                                                             <  1:185458/139‑1 (MQ=255)

                  aaTGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACAGTtgtg                                                                                                                   >  1:439926/1‑139 (MQ=255)

                            cggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGAtaaga                                                                                                         >  1:870991/1‑135 (MQ=255)

                            cggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGAtaaga                                                                                                         >  1:440695/1‑135 (MQ=255)

                                 aGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                     <  2:396120/106‑1 (MQ=255)

                                 aGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                     >  1:396120/1‑106 (MQ=255)

                                   tGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGa                                                                                                  <  2:1248112/139‑1 (MQ=255)

                                   tGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGa                                                                                                  <  2:578699/139‑1 (MQ=255)

                                   tGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGa                                                                                                  <  2:906176/139‑1 (MQ=255)

                                   tGGTTATGTCAGAAGCCGTGGGAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGa                                                                                                  <  2:870991/139‑1 (MQ=255)

                                    ggTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGAc                                                                                                 >  1:1437137/1‑139 (MQ=255)

                                           tCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCtt                                                                                          >  2:150474/1‑139 (MQ=255)

                                             aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAg                                                                                                                            >  1:657123/1‑103 (MQ=255)

                                             aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAg                                                                                                                            <  2:657123/103‑1 (MQ=255)

                                             aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTAt                                                                                        >  1:332574/1‑139 (MQ=255)

                                                               gcgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc                                                                                       <  2:1309825/122‑1 (MQ=255)

                                                               gcgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc                                                                                       >  1:1309825/1‑122 (MQ=255)

                                                                cgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGcc                                                                     >  2:1470992/1‑139 (MQ=255)

                                                                  tGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGc                                                                   >  1:1211433/1‑139 (MQ=255)

                                                                          ggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                     >  1:148966/1‑65 (MQ=255)

                                                                          ggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                     <  2:148966/65‑1 (MQ=255)

                                                                                           aaGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCa                                                                                      <  1:1181853/95‑1 (MQ=255)

                                                                                           aaGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCa                                                                                      >  2:1181853/1‑95 (MQ=39)

                                                                                                aCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGAt                                                                                                             >  1:450884/1‑67 (MQ=255)

                                                                                                aCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGAt                                                                                                             <  2:450884/67‑1 (MQ=255)

                                                                                                 cGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc                                                                                       >  1:1350832/1‑88 (MQ=39)

                                                                                                 cGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc                                                                                       <  2:1350832/88‑1 (MQ=39)

                                                                                                 cGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcg                                    >  2:1293573/1‑139 (MQ=255)

                                                                                                   cTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCaa                                                          <  2:1020011/115‑1 (MQ=255)

                                                                                                   cTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCaa                                                          >  1:1020011/1‑115 (MQ=255)

                                                                                                   cTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcgat                                  >  2:880341/1‑139 (MQ=255)

                                                                                                    tGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcgatg                                 <  1:1293573/139‑1 (MQ=255)

                                                                                                     ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                     >  2:1371470/1‑38 (MQ=255)

                                                                                                     ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                     <  1:1371470/38‑1 (MQ=255)

                                                                                                         ggcgAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                            <  2:1211433/139‑1 (MQ=255)

                                                                                                              aGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc                                                                                       <  2:931190/75‑1 (MQ=38)

                                                                                                              aGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc                                                                                       >  1:931190/1‑75 (MQ=38)

                                                                                                                        tGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  >  1:916686/1‑134 (MQ=255)

                                                                                                                        tGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  <  2:916686/134‑1 (MQ=255)

                                                                                                                          tATTTCGCTGTAGTCGTAGGGATTACACATTTGTGCCAGATAAGACGGTTGCCGCGTATTATCAGGCCTACAACCGGGGCCGCATCCGGCAATTGGTGCACAATGACTGATGCGATGCTTGACGCATCTTATCAGGCCt           <  1:1470992/139‑1 (MQ=37)

                                                                                                                               cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAAcc                                                                           >  2:470327/1‑70 (MQ=25)

                                                                                                                               cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAAcc                                                                           <  1:470327/70‑1 (MQ=25)

                                                                                                                                 cTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  <  2:4517/125‑1 (MQ=255)

                                                                                                                                 cTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  >  1:4517/1‑125 (MQ=255)

                                                                                                                                 cTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATgg    >  1:1155676/1‑139 (MQ=255)

                                                                                                                                   gTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGt  <  2:1437137/139‑1 (MQ=255)

GATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGT  >  NC_000913/551198‑551467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A