Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	551,332	G→A	298 (TAG→TAA)	ybcF →	putative carbonate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	551,332	0	G	A	100.0%	96.3 / NA	32	298 (TAG→TAA)	ybcF	putative carbonate kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/19); total (13/19)

GATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGG  >  NC_000913/551198‑551466

gATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                    <  1:296023/139‑1 (MQ=255)

gATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                    <  2:125253/139‑1 (MQ=255)

 aTGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTa                                                                                                                                   <  1:333934/139‑1 (MQ=255)

                        aCGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGAt                                                                                                            >  1:577806/1‑139 (MQ=255)

                         cggcggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATg                                                                                                           <  1:13737/139‑1 (MQ=255)

                          ggcggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGc                                                                                                          >  2:1072426/1‑139 (MQ=255)

                                   tGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                 <  2:267400/139‑1 (MQ=255)

                                   tGGTTAGGTCAGAAGCCGTGGTAAACCCGCGGGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGTAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                 <  2:851984/139‑1 (MQ=255)

                                                aGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                       <  2:421430/136‑1 (MQ=255)

                                                aGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                       >  1:421430/1‑136 (MQ=255)

                                                aGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                    >  2:248079/1‑139 (MQ=255)

                                                   cGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCt                                                                                                               >  2:315713/1‑109 (MQ=255)

                                                   cGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCt                                                                                                               <  1:315713/109‑1 (MQ=255)

                                                    gTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCa                                                                                     <  2:465242/134‑1 (MQ=255)

                                                    gTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCa                                                                                     >  1:465242/1‑134 (MQ=255)

                                                                         gggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGc                                                           <  1:1072426/139‑1 (MQ=255)

                                                                            cGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt                                                        <  1:506326/139‑1 (MQ=255)

                                                                               tATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTgg                                                     <  2:577806/139‑1 (MQ=255)

                                                                               tATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTGTTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTgg                                                     >  2:1056559/1‑139 (MQ=255)

                                                                                                   cTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcgat                                 >  1:912536/1‑139 (MQ=255)

                                                                                                     ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGa                                                                                                                          <  2:262823/48‑1 (MQ=255)

                                                                                                     ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGa                                                                                                                          >  1:262823/1‑48 (MQ=255)

                                                                                                                   ggACTTTTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGGGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                 <  2:551425/139‑1 (MQ=255)

                                                                                                                   ggACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                 <  1:765992/139‑1 (MQ=255)

                                                                                                                   ggACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                 <  1:339586/139‑1 (MQ=255)

                                                                                                                   ggACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                 <  1:248079/139‑1 (MQ=255)

                                                                                                                      ccTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAg              <  1:271099/139‑1 (MQ=255)

                                                                                                                          tATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt                                                        >  2:941601/1‑93 (MQ=255)

                                                                                                                          tATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt                                                        <  1:941601/93‑1 (MQ=255)

                                                                                                                                 cTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATgg   >  2:1045600/1‑139 (MQ=255)

                                                                                                                                 cTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATgg   >  2:1045599/1‑139 (MQ=255)

                                                                                                                                  tGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATggg  >  2:1001279/1‑139 (MQ=255)

GATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGG  >  NC_000913/551198‑551466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A