Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,307	C→T	47 (TAG→TAA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,307	0	C	T	100.0%	197.7 / NA	61	47 (TAG→TAA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/31); total (30/31)

CTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAA  >  NC_000913/1119175‑1119424

cTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaaaa                                                                                                                 >  1:963075/1‑139 (MQ=255)

 ttCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAAt                                                                                                                <  1:456438/139‑1 (MQ=255)

 ttCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAAt                                                                                                                >  1:254087/1‑139 (MQ=255)

 ttCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAAt                                                                                                                >  1:23297/1‑139 (MQ=255)

  tCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                               >  2:383835/1‑139 (MQ=255)

   cATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                               >  1:1018236/1‑138 (MQ=255)

   cATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                               <  2:1018236/138‑1 (MQ=255)

    aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                             <  2:310835/139‑1 (MQ=255)

           tAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATa                                                                                                      >  1:491585/1‑139 (MQ=255)

           tAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATa                                                                                                      >  2:1070631/1‑139 (MQ=255)

              tGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                   <  2:628754/139‑1 (MQ=255)

              tGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                   <  1:383835/139‑1 (MQ=255)

                 gTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagatcag                                                                                                <  2:963075/139‑1 (MQ=255)

                   tCAGCATCAGAGATCTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAATGGGAGATTAAATATTTGTCAGTTTATCTCAGTGCGGAAAATTAAAAATAAAACACATagatcagat                                                                                              >  2:894113/1‑139 (MQ=255)

                                 gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGGCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGAGCCATAATTGCcaac                                                                                <  2:992980/139‑1 (MQ=255)

                                    tGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACAt                                                                                                       <  1:16695/113‑1 (MQ=255)

                                    tGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACAt                                                                                                       >  2:16695/1‑113 (MQ=255)

                                       tGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaa                                                                                                              <  2:207120/103‑1 (MQ=255)

                                       tGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaa                                                                                                              >  1:207120/1‑103 (MQ=255)

                                           tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                             <  1:206371/100‑1 (MQ=255)

                                           tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                             >  2:206371/1‑100 (MQ=255)

                                           tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCaga                                                                      <  2:23297/139‑1 (MQ=255)

                                            gCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCagag                                                                     >  1:715630/1‑139 (MQ=255)

                                              tGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaa                                                                   >  2:1005489/1‑139 (MQ=255)

                                                tAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaaaa                                                                 >  2:358622/1‑139 (MQ=255)

                                                     gCAGGAATCATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                            <  1:894113/139‑1 (MQ=255)

                                                     gCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaaa                                                                                                                  <  1:1124549/85‑1 (MQ=255)

                                                     gCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaaa                                                                                                                  >  2:1124549/1‑85 (MQ=255)

                                                     gCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                   >  2:1416087/1‑100 (MQ=255)

                                                     gCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                   <  1:1416087/100‑1 (MQ=255)

                                                     gCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                            <  2:491585/139‑1 (MQ=255)

                                                      cAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                           <  1:358622/139‑1 (MQ=255)

                                                       aGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaaa                                                          <  1:1070631/139‑1 (MQ=255)

                                                              atCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                             <  1:1430352/81‑1 (MQ=255)

                                                              atCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                             >  2:1430352/1‑81 (MQ=255)

                                                                 tcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATg                                                <  1:819079/139‑1 (MQ=255)

                                                                                  cTAAATGAAAGGTAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtga                               <  2:68241/139‑1 (MQ=255)

                                                                                  cTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtga                               <  2:254087/139‑1 (MQ=255)

                                                                                   tAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATccc                                                                          <  2:1459976/95‑1 (MQ=255)

                                                                                   tAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATccc                                                                          >  1:1459976/1‑95 (MQ=255)

                                                                                           aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAAcaca                                                                                                        >  2:838887/1‑57 (MQ=255)

                                                                                           aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAAcaca                                                                                                        <  1:996184/57‑1 (MQ=255)

                                                                                           aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAAcaca                                                                                                        <  1:838887/57‑1 (MQ=255)

                                                                                           aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAAcaca                                                                                                        >  2:996184/1‑57 (MQ=255)

                                                                                               agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                            <  2:181097/97‑1 (MQ=255)

                                                                                               agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                            >  1:181097/1‑97 (MQ=255)

                                                                                               agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCaa                  >  2:726643/1‑139 (MQ=255)

                                                                                               agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCaa                  >  2:722485/1‑139 (MQ=255)

                                                                                                gaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCcaaca                                                                               <  2:815114/77‑1 (MQ=255)

                                                                                                gaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCcaaca                                                                               >  1:815114/1‑77 (MQ=255)

                                                                                                 aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACa                >  2:1170414/1‑139 (MQ=255)

                                                                                                      atatTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGa           <  1:1005489/139‑1 (MQ=255)

                                                                                                        atTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACg         >  1:1456840/1‑139 (MQ=255)

                                                                                                           tGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                           >  2:1258114/1‑86 (MQ=255)

                                                                                                           tGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                           <  1:1258114/86‑1 (MQ=255)

                                                                                                           tGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCAt      >  1:976888/1‑139 (MQ=255)

                                                                                                            gTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgcg                                          >  2:1023181/1‑102 (MQ=255)

                                                                                                            gTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgcg                                          <  1:1023181/102‑1 (MQ=255)

                                                                                                               aGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTaaa  <  2:715630/139‑1 (MQ=255)

                                                                                                                           tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatga                            >  1:975483/1‑101 (MQ=255)

                                                                                                                           tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatga                            <  2:975483/101‑1 (MQ=255)

CTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAA  >  NC_000913/1119175‑1119424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A