Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,307	C→T	47 (TAG→TAA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,307	0	C	T	100.0%	184.0 / NA	57	47 (TAG→TAA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/27); total (30/27)

AATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGG  >  NC_000913/1119172‑1119441

aaTCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaa                                                                                                                                     >  2:1443268/1‑139 (MQ=255)

     tCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                                                <  1:1535624/139‑1 (MQ=255)

              tAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATa                                                                                                                       >  1:966828/1‑139 (MQ=255)

                 tGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGACAGGGAGATTAAATAGTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                    <  1:1443268/139‑1 (MQ=255)

                 tGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                    <  1:1225537/139‑1 (MQ=255)

                     ttCAGCTTCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                    >  1:565235/1‑135 (MQ=255)

                     ttCAGCTTCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                    <  2:565235/135‑1 (MQ=255)

                     ttCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                    <  2:541327/135‑1 (MQ=255)

                     ttCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                    >  1:541327/1‑135 (MQ=255)

                               gagaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                    <  2:599103/125‑1 (MQ=255)

                               gagaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                    >  1:599103/1‑125 (MQ=255)

                               gagaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTg                                                                                                      >  2:636875/1‑139 (MQ=255)

                                  aTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTCTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCca                                                                                                   >  1:619201/1‑139 (MQ=255)

                                    gTCTGGTGAGTGCTGTAGAAGCAGAAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCcaac                                                                                                 >  2:1122582/1‑139 (MQ=255)

                                          tGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATccc                                                                                           >  1:124875/1‑139 (MQ=255)

                                            aGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                              >  1:335721/1‑102 (MQ=255)

                                            aGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                              <  2:335721/102‑1 (MQ=255)

                                              tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCaga                                                                                       >  1:555551/1‑139 (MQ=255)

                                              tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCaga                                                                                       >  1:758187/1‑139 (MQ=255)

                                                cTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaa                                                                                                                                     >  1:729866/1‑91 (MQ=255)

                                                cTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaa                                                                                                                                     <  2:729866/91‑1 (MQ=255)

                                                cTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAgaga                                                                                     >  1:381060/1‑139 (MQ=255)

                                                 tGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaa                                                                                    <  2:381060/139‑1 (MQ=255)

                                                   tAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaaaa                                                                                  >  1:110141/1‑139 (MQ=255)

                                                     gAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAg                                                                                <  2:555551/139‑1 (MQ=255)

                                                        gCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                                             >  1:1225193/1‑139 (MQ=255)

                                                           ggAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCa                                                                                         <  1:263353/124‑1 (MQ=255)

                                                           ggAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCa                                                                                         >  2:263353/1‑124 (MQ=255)

                                                                  tctcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGa                                                                   >  1:520185/1‑139 (MQ=255)

                                                                      aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCt                                                               >  2:97257/1‑139 (MQ=255)

                                                                          aaTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgcg                                                           <  2:758187/139‑1 (MQ=255)

                                                                          aaTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgcg                                                           <  2:124875/139‑1 (MQ=255)

                                                                            tttttCACGCTAAATGAAAGGGAGATTAAGTATTTGTCAGTTTATCTCAGTGCGGAAAATTAAAAATaaa                                                                                                                              >  2:957661/1‑70 (MQ=255)

                                                                            tttttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                              <  1:957661/70‑1 (MQ=255)

                                                                              tttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAATGCATCAGATGCTCGCGAata                                                       <  1:1122582/139‑1 (MQ=255)

                                                                                  aCGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCAGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAAGCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtattat                                                   <  2:409997/139‑1 (MQ=255)

                                                                                          tGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATgaga                                           <  2:520185/139‑1 (MQ=255)

                                                                                                ggAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatga                                             >  2:1185686/1‑131 (MQ=255)

                                                                                                ggAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatga                                             <  1:1185686/131‑1 (MQ=255)

                                                                                                    aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGc                                                                                                     >  2:481654/1‑71 (MQ=255)

                                                                                                    aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGc                                                                                                     <  1:481654/71‑1 (MQ=255)

                                                                                                    aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                                            >  2:1073548/1‑96 (MQ=255)

                                                                                                    aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                                            <  1:1073548/96‑1 (MQ=255)

                                                                                                    aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatg                                              >  2:970366/1‑126 (MQ=255)

                                                                                                    aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatg                                              <  1:970366/126‑1 (MQ=255)

                                                                                                           atTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACg                          >  2:735613/1‑139 (MQ=255)

                                                                                                             ttGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAAATGCCTACATTCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCa                        <  2:1225193/139‑1 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCGAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa       >  2:1029392/1‑139 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaa                                                                                    >  1:818902/1‑62 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaa                                                                                    <  2:818902/62‑1 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                                            >  2:1194605/1‑70 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                                            <  1:1194605/70‑1 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTc                                                              <  2:300183/84‑1 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTc                                                              >  1:300183/1‑84 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa       >  1:293605/1‑139 (MQ=255)

                                                                                                                                cGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaaaa     <  1:636875/139‑1 (MQ=255)

                                                                                                                                   aaaaTTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACgg  <  1:735613/139‑1 (MQ=255)

AATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGG  >  NC_000913/1119172‑1119441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A