Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,307	C→T	47 (TAG→TAA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,307	0	C	T	100.0%	141.2 / NA	45	47 (TAG→TAA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/21); total (24/21)

AATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGC  >  NC_000913/1119172‑1119442

aaTCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaa                                                                                                                                        >  1:430197/1‑139 (MQ=255)

   cTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaaaa                                                                                                                                     >  1:1788371/1‑139 (MQ=255)

       aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                                 <  2:430197/139‑1 (MQ=255)

                 tGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagat                                                                                                                     <  1:1242121/139‑1 (MQ=255)

                 tGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagat                                                                                                                     >  2:1339509/1‑139 (MQ=255)

                      tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                                                   <  1:10859/122‑1 (MQ=255)

                      tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                                                   >  2:10859/1‑122 (MQ=255)

                      tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑Acaca                                                                                                                          >  1:1022452/1‑129 (MQ=255)

                      tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑Acaca                                                                                                                          <  2:1022452/129‑1 (MQ=255)

                       cAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagatcagatc                                                                                                               >  1:829143/1‑139 (MQ=255)

                                agaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGc                                                                                                      <  1:1339509/139‑1 (MQ=255)

                                agaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGc                                                                                                      <  2:522397/139‑1 (MQ=255)

                                  aTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCca                                                                                                    >  2:778300/1‑139 (MQ=255)

                                    gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACACATAGATCAGATCCATAATTGCca                                                                                                    >  2:1308066/1‑139 (MQ=255)

                                    gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCcaac                                                                                                  >  2:278937/1‑139 (MQ=255)

                                    gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCcaac                                                                                                  >  1:588174/1‑139 (MQ=255)

                                            aGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaa                                                                                                                                  <  1:940294/101‑1 (MQ=255)

                                            aGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaa                                                                                                                                  >  2:940294/1‑101 (MQ=255)

                                              tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCa                                                                                          <  1:456968/137‑1 (MQ=255)

                                              tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCa                                                                                          >  2:456968/1‑137 (MQ=255)

                                                  gTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaaa                                                                                    <  2:1788371/139‑1 (MQ=255)

                                                             aataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagatcag                                                                                                                  <  2:1420955/98‑1 (MQ=255)

                                                             aataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagatcag                                                                                                                  >  1:1420955/1‑98 (MQ=255)

                                                                  tctcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGa                                                                    >  1:255564/1‑139 (MQ=255)

                                                                  tctcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGa                                                                    >  2:1485604/1‑139 (MQ=255)

                                                                    tcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATg                                                                  <  1:278937/139‑1 (MQ=255)

                                                                            tttttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                          <  1:498638/139‑1 (MQ=255)

                                                                                    gCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtg                                                  <  2:588174/139‑1 (MQ=255)

                                                                                              aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatt                                                      <  1:446874/125‑1 (MQ=255)

                                                                                              aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGAATCAGATGCTCGCGAAtatt                                                      >  2:446874/1‑125 (MQ=255)

                                                                                                  agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑acac                                                                                                                           >  2:1275287/1‑52 (MQ=255)

                                                                                                  agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑acac                                                                                                                           <  1:1275287/52‑1 (MQ=255)

                                                                                                        aaTATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCg                              <  2:255564/139‑1 (MQ=255)

                                                                                                                  aGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCCGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                             >  2:1203922/1‑98 (MQ=255)

                                                                                                                  aGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                             <  1:1203922/98‑1 (MQ=255)

                                                                                                                            aGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTa          >  1:1039809/1‑139 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                                              <  1:1414568/69‑1 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                                              >  2:1414568/1‑69 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                          >  2:1518580/1‑89 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                          <  1:1518580/89‑1 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTaaa                    <  2:1611370/127‑1 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTaaa                    >  1:1611370/1‑127 (MQ=255)

                                                                                                                              tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa        >  2:1548894/1‑139 (MQ=255)

                                                                                                                                cGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaaaa      >  1:1785156/1‑139 (MQ=255)

                                                                                                                                    aaaTTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGc  <  2:1785156/139‑1 (MQ=255)

AATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGC  >  NC_000913/1119172‑1119442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A