Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I2 R1 777 59.2 2955938 92.5% 2734242 106.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,493,219 A→G E250E (GAA→GAG)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,493,2190AG100.0% 210.3 / NA 59E250E (GAA→GAG) trgmethyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/31);  total (28/31)

GCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGG  >  NC_000913/1493086‑1493353
                                                                                                                                     |                                                                                                                                      
gcgcGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                                   <  2:921209/139‑1 (MQ=255)
gcgcGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                                   <  1:318283/139‑1 (MQ=255)
 cgcgTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggcg                                                                                                                                  <  1:1144057/139‑1 (MQ=255)
 cgcgTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggcg                                                                                                                                  >  1:636695/1‑139 (MQ=255)
    gTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                               <  2:847003/139‑1 (MQ=255)
    gTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                               <  2:801515/139‑1 (MQ=255)
               cccTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAAt                                                                                                                    >  2:580988/1‑139 (MQ=255)
                   ggTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt                                                                                                                >  1:648718/1‑139 (MQ=255)
                   ggTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt                                                                                                                >  1:41513/1‑139 (MQ=255)
                              tGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                                   >  2:558355/1‑109 (MQ=255)
                              tGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                                   <  1:558355/109‑1 (MQ=255)
                              tGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcg                                                                                                     >  1:510201/1‑139 (MQ=255)
                               gATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcgt                                                                                                    <  1:1188664/139‑1 (MQ=255)
                                               cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                                   <  2:1087575/92‑1 (MQ=255)
                                               cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                                   >  1:1087575/1‑92 (MQ=255)
                                               cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                               <  2:757177/96‑1 (MQ=255)
                                               cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                               >  1:757177/1‑96 (MQ=255)
                                               cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgc                                                                                    >  1:371456/1‑139 (MQ=255)
                                               cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgc                                                                                    >  1:1166226/1‑139 (MQ=255)
                                                tACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                      >  2:866210/1‑136 (MQ=255)
                                                tACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                      <  1:866210/136‑1 (MQ=255)
                                                  cGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagc                                                                                 >  2:970777/1‑139 (MQ=255)
                                                   gTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca                                                                                <  2:41513/139‑1 (MQ=255)
                                                       gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCa                                                                            <  2:371456/139‑1 (MQ=255)
                                                       gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCa                                                                            <  1:580988/139‑1 (MQ=255)
                                                       gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCa                                                                            <  2:648718/139‑1 (MQ=255)
                                                         tCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                          <  2:1166226/139‑1 (MQ=255)
                                                         tCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                          <  1:847786/139‑1 (MQ=255)
                                                            tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTggg                                                                       <  2:510201/139‑1 (MQ=255)
                                                            tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTggg                                                                       >  2:267193/1‑139 (MQ=255)
                                                            tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTggg                                                                       >  2:230486/1‑139 (MQ=255)
                                                              aTTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGa                                                                     <  2:636695/139‑1 (MQ=255)
                                                              aTTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGa                                                                     <  1:230486/139‑1 (MQ=255)
                                                                tCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg                                                                   <  2:510231/139‑1 (MQ=255)
                                                                    ccACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACa                                                                                            >  1:1370478/1‑110 (MQ=255)
                                                                    ccACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACa                                                                                            <  2:1370478/110‑1 (MQ=255)
                                                                            aCATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                          >  1:1167207/1‑120 (MQ=255)
                                                                            aCATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                          <  2:1167207/120‑1 (MQ=255)
                                                                             cATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                      <  1:679996/107‑1 (MQ=255)
                                                                             cATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                      >  2:679996/1‑107 (MQ=255)
                                                                             cATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTg                                                      >  1:355788/1‑139 (MQ=255)
                                                                               tGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                                   <  2:74003/60‑1 (MQ=255)
                                                                               tGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                                   >  1:74003/1‑60 (MQ=255)
                                                                                         cGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGgcg                                          >  2:1444047/1‑139 (MQ=255)
                                                                                              tGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                      >  1:494836/1‑90 (MQ=255)
                                                                                              tGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                      <  2:494836/90‑1 (MQ=255)
                                                                                                     aTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGAttt                              >  2:1239395/1‑139 (MQ=255)
                                                                                                               gCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCAc                    >  1:529206/1‑139 (MQ=255)
                                                                                                                cGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg                                                                                                                  >  2:1431472/1‑44 (MQ=255)
                                                                                                                cGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg                                                                                                                  <  1:1431472/44‑1 (MQ=255)
                                                                                                                  aTCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAg                 <  1:1239395/139‑1 (MQ=255)
                                                                                                                         gATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAg                                                                                           <  2:1198802/58‑1 (MQ=255)
                                                                                                                         gATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAg                                                                                           >  1:1198802/1‑58 (MQ=255)
                                                                                                                            gaatgaTGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCa       <  2:227433/139‑1 (MQ=255)
                                                                                                                              atgatgAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAgg                                                           >  1:934679/1‑85 (MQ=255)
                                                                                                                              atgatgAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAgg                                                           <  2:934679/85‑1 (MQ=255)
                                                                                                                               tgatgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCt    <  2:699188/139‑1 (MQ=255)
                                                                                                                                 atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTgg  >  1:1235117/1‑139 (MQ=255)
                                                                                                                                 atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTgg  >  1:1423638/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGG  >  NC_000913/1493086‑1493353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A