Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,493,219 |
A→G |
E250E (GAA→GAG) |
trg → |
methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,493,219 | 0 | A | G | 100.0%
| 154.7
/ NA
| 43 | E250E (GAA→GAG) | trg | methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/22); total (21/22) |
GTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGC > NC_000913/1493090‑1493354
|
gTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt < 1:944217/139‑1 (MQ=255)
gtgCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTa < 2:1076969/139‑1 (MQ=255)
gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTa > 2:705427/1‑139 (MQ=255)
cTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtc < 2:217638/139‑1 (MQ=255)
ttATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACa > 2:370337/1‑139 (MQ=255)
tGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt < 1:1631781/116‑1 (MQ=255)
tGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt > 2:1631781/1‑116 (MQ=255)
cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg < 1:1576199/119‑1 (MQ=255)
cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg > 2:1576199/1‑119 (MQ=255)
gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcgg > 2:846530/1‑83 (MQ=255)
gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcgg < 1:846530/83‑1 (MQ=255)
gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCa > 1:185872/1‑139 (MQ=255)
gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCa < 2:1362188/139‑1 (MQ=255)
gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCa > 1:1353521/1‑139 (MQ=255)
cGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTg < 2:1353521/139‑1 (MQ=255)
tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTggg > 2:6812/1‑139 (MQ=255)
tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTggg > 2:1384483/1‑139 (MQ=255)
tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTc > 2:1666764/1‑72 (MQ=255)
tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTc < 1:1666764/72‑1 (MQ=255)
tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAgg < 2:798477/139‑1 (MQ=255)
cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt > 1:1328795/1‑69 (MQ=255)
cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt < 2:1328795/69‑1 (MQ=255)
cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg < 2:1172851/82‑1 (MQ=255)
cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg > 1:1172851/1‑82 (MQ=255)
cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGa > 2:1195732/1‑139 (MQ=255)
cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGa > 1:1637212/1‑139 (MQ=255)
cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGa < 2:185872/139‑1 (MQ=255)
aCGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGgc < 2:1637212/139‑1 (MQ=255)
aaaaaTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGa < 2:197667/116‑1 (MQ=255)
aaaaaTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGa > 1:197667/1‑116 (MQ=255)
aaaTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCGTAAGTCGTAATTGACAGCAAATGCGGAATTCACTGGGGGTGACAGTAGGGACAGGTCGACAGGGCGTGGAAgagag > 2:762724/1‑138 (MQ=255)
tCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt < 2:168029/56‑1 (MQ=255)
tCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt > 1:168029/1‑56 (MQ=255)
cAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCgg < 2:1587731/51‑1 (MQ=255)
cAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCgg > 1:1587731/1‑51 (MQ=255)
aTCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg < 1:315630/89‑1 (MQ=255)
aTCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg > 2:315630/1‑89 (MQ=255)
cTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCg < 1:705427/139‑1 (MQ=255)
tgatgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgca < 2:395835/60‑1 (MQ=255)
tgatgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgca > 1:395835/1‑60 (MQ=255)
gatgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTg < 1:1384483/139‑1 (MQ=255)
atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTgg < 1:370337/139‑1 (MQ=255)
tgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGc > 1:728825/1‑139 (MQ=255)
|
GTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGC > NC_000913/1493090‑1493354
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
