Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,910,474	T→C	S67P (TCC→CCC)	yebQ →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,910,474	0	T	C	100.0%	175.8 / NA	50	S67P (TCC→CCC)	yebQ	putative transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/27); total (23/27)

ATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGT  >  NC_000913/1910342‑1910608

aTTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCCCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGc                                                                                                                                  >  2:1457478/1‑139 (MQ=255)

          cGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCtcgttttc                                                                                                                        >  1:1342032/1‑139 (MQ=255)

               gCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTc                                                                                                                   >  2:134452/1‑139 (MQ=255)

               gCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTc                                                                                                                   >  1:1259227/1‑139 (MQ=255)

               gCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTACCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTc                                                                                                                   >  1:1245274/1‑139 (MQ=255)

                ccGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCt                                                                                                                  <  1:364942/139‑1 (MQ=255)

                   tCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTggg                                                                                                               >  2:1062789/1‑139 (MQ=255)

                   tCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTggg                                                                                                               >  1:80942/1‑139 (MQ=255)

                     cTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                             <  2:83911/139‑1 (MQ=255)

                     cTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                             <  2:1342032/139‑1 (MQ=255)

                       tGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGat                                                                                                           >  1:65311/1‑139 (MQ=255)

                            gcgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCACCCCCCTGCTCTCGCTTTCGTTTCTGGGCGATATGtt                                                                                                      <  2:1139848/139‑1 (MQ=255)

                             cgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGtt                                                                                                      >  1:1370789/1‑138 (MQ=255)

                             cgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGtt                                                                                                      <  2:1370789/138‑1 (MQ=255)

                               cAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTgg                                                                                                   <  2:488983/139‑1 (MQ=255)

                                     ccAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCg                                                                                             <  2:343714/139‑1 (MQ=255)

                                     ccAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCg                                                                                             <  1:1062789/139‑1 (MQ=255)

                                       aaCGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                           <  2:80942/139‑1 (MQ=255)

                                          gTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTa                                                                                        <  2:65311/139‑1 (MQ=255)

                                              cccTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                           >  1:721253/1‑132 (MQ=255)

                                              cccTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                           <  2:721253/132‑1 (MQ=255)

                                              cccTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTta                                                                                    >  1:210816/1‑139 (MQ=255)

                                                        aaTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGt                                                                          <  2:474388/139‑1 (MQ=255)

                                                         aTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTc                                                                         >  2:723621/1‑139 (MQ=255)

                                                             ccACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGt                                                                     <  2:210816/139‑1 (MQ=255)

                                                             ccACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGt                                                                     >  1:792766/1‑139 (MQ=255)

                                                                      ttCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCt                                                            >  2:1101612/1‑139 (MQ=255)

                                                                       tCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtg                                                           <  2:792766/139‑1 (MQ=255)

                                                                        cATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgt                                                          <  1:1101612/139‑1 (MQ=255)

                                                                         aTGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgtt                                                         <  1:134452/139‑1 (MQ=255)

                                                                             cACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGt                                                                                         <  2:143215/103‑1 (MQ=255)

                                                                             cACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGt                                                                                         >  1:143215/1‑103 (MQ=255)

                                                                             cACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATg                                                                              <  1:1063083/114‑1 (MQ=255)

                                                                             cACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATg                                                                              >  2:1063083/1‑114 (MQ=255)

                                                                                         ttCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGTTATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTgc                                         <  1:723621/139‑1 (MQ=255)

                                                                                         ttCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTgc                                         <  2:1245274/139‑1 (MQ=255)

                                                                                           ccATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTgcgc                                       <  2:13890/139‑1 (MQ=255)

                                                                                                       tGAACGCCTATCAAATCGCCATTGTCATCCCCCTGc                                                                                                                                  >  2:246626/1‑36 (MQ=255)

                                                                                                       tGAACGCCTATCAAATCGCCATTGTCATCCCCCTGc                                                                                                                                  <  1:246626/36‑1 (MQ=255)

                                                                                                         aaCGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTc                           >  2:1229460/1‑137 (MQ=255)

                                                                                                         aaCGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTc                           <  1:1229460/137‑1 (MQ=255)

                                                                                                            gCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                             <  2:739519/52‑1 (MQ=255)

                                                                                                            gCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                             >  1:739519/1‑52 (MQ=255)

                                                                                                            gCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGc                      >  2:374839/1‑139 (MQ=255)

                                                                                                              cTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCaa                    >  1:1189920/1‑139 (MQ=255)

                                                                                                              cTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCaa                    <  1:374839/139‑1 (MQ=255)

                                                                                                                 tCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATg                 >  1:293061/1‑139 (MQ=255)

                                                                                                                             tGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCa                                                  <  1:1138275/94‑1 (MQ=255)

                                                                                                                             tGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCa                                                  >  2:1138275/1‑94 (MQ=255)

                                                                                                                                cATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCgt  <  2:293061/139‑1 (MQ=255)

ATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGT  >  NC_000913/1910342‑1910608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A