Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,910,474	T→C	S67P (TCC→CCC)	yebQ →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,910,474	0	T	C	100.0%	188.1 / NA	53	S67P (TCC→CCC)	yebQ	putative transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/28); total (25/28)

TGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCG  >  NC_000913/1910340‑1910607

tgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCt                                                                                                                                   >  1:411322/1‑139 (MQ=255)

   ttGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGct                                                                                                                                <  2:303391/139‑1 (MQ=255)

    tGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGctc                                                                                                                               >  1:500548/1‑139 (MQ=255)

              aTGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCtcgttttcgt                                                                                                                     >  1:1176413/1‑139 (MQ=255)

                ggCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCtcgttttcgttt                                                                                                                   >  2:188629/1‑139 (MQ=255)

                 gCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTc                                                                                                                  >  2:796857/1‑139 (MQ=255)

                 gCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTc                                                                                                                  >  2:12105/1‑139 (MQ=255)

                  ccGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCt                                                                                                                 <  1:12105/139‑1 (MQ=255)

                     tCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTggg                                                                                                              >  1:224379/1‑139 (MQ=255)

                       cTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                            <  2:411322/139‑1 (MQ=255)

                       cTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                            <  1:1524221/139‑1 (MQ=255)

                       cTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                            <  1:29666/139‑1 (MQ=255)

                        ttGACGGCGCAATCGCCAACGTCGCCCTACCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGa                                                                                                           <  2:238645/139‑1 (MQ=255)

                         tGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGat                                                                                                          <  2:1538772/139‑1 (MQ=255)

                              gcgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGtt                                                                                                     <  2:1233526/139‑1 (MQ=255)

                               cgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGttt                                                                                                    >  1:275373/1‑139 (MQ=255)

                               cgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGttt                                                                                                    >  1:877769/1‑139 (MQ=255)

                                    tCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTa                                                                                               >  2:402822/1‑139 (MQ=255)

                                     cGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTAt                                                                                              <  2:1407989/139‑1 (MQ=255)

                                     cGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGCTCCATCTGGGTAGTGAACGCCTATCAAATAGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTAt                                                                                              <  1:1060602/139‑1 (MQ=255)

                                         aaCGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                          <  2:275373/139‑1 (MQ=255)

                                         aaCGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                          <  2:1438532/139‑1 (MQ=255)

                                         aaCGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                          <  2:137498/139‑1 (MQ=255)

                                         aaCGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                          <  2:877769/139‑1 (MQ=255)

                                           cGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGt                                                                                        <  2:346458/139‑1 (MQ=255)

                                              cGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTAtt                                                                                     >  2:428056/1‑139 (MQ=255)

                                              cGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCAGCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTAtt                                                                                     >  2:1065117/1‑139 (MQ=255)

                                              cGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCAGCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTCGGCTATCGACGTAtt                                                                                     >  2:1065116/1‑139 (MQ=255)

                                                cccTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTa                                                                                       <  1:937674/135‑1 (MQ=255)

                                                cccTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTa                                                                                       >  2:937674/1‑135 (MQ=255)

                                                cccTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTta                                                                                   <  1:796857/139‑1 (MQ=255)

                                                          aaTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGt                                                                         <  2:224379/139‑1 (MQ=255)

                                                           aTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTgg                                                                                                               <  2:152721/100‑1 (MQ=255)

                                                           aTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTgg                                                                                                               >  1:152721/1‑100 (MQ=255)

                                                                    gACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGttt                                                               >  2:825182/1‑139 (MQ=255)

                                                                           aTGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgtt                                                        <  2:536543/139‑1 (MQ=255)

                                                                                 cGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                          <  2:613798/99‑1 (MQ=255)

                                                                                 cGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                          >  1:613798/1‑99 (MQ=255)

                                                                                    cagccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACt                                               >  1:793587/1‑139 (MQ=255)

                                                                                                tCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCt                                   >  2:1173469/1‑139 (MQ=255)

                                                                                                    ggTAGTGAACGCCTATCAAATTGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                          <  1:1180645/80‑1 (MQ=255)

                                                                                                    ggTAGTGAACGCCTATCAAATCGCCATTGTAATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                          >  2:1180645/1‑80 (MQ=255)

                                                                                                           aaCGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGc                        >  1:280403/1‑139 (MQ=255)

                                                                                                              gCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGc                     <  2:793587/139‑1 (MQ=255)

                                                                                                                          gCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCAc                                                <  1:1550547/100‑1 (MQ=255)

                                                                                                                          gCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCAc                                                >  2:1550547/1‑100 (MQ=255)

                                                                                                                           ccATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCt        >  2:916161/1‑139 (MQ=255)

                                                                                                                             aTTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTg      >  2:1543561/1‑139 (MQ=255)

                                                                                                                              ttGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTgc     <  2:280403/139‑1 (MQ=255)

                                                                                                                              ttGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTgc     <  1:1445261/139‑1 (MQ=255)

                                                                                                                               tGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCAcc          >  1:161489/1‑133 (MQ=255)

                                                                                                                               tGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCAcc          <  2:161489/133‑1 (MQ=255)

                                                                                                                                 tCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGcgcg  <  1:428056/139‑1 (MQ=255)

TGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCG  >  NC_000913/1910340‑1910607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A