Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,012,700	C→T	105 (TAG→TAA)	fliE ←	flagellar basal‑body component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,012,700	0	C	T	100.0%	148.7 / NA	47	105 (TAG→TAA)	fliE	flagellar basal‑body component
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/28); total (19/28)

TTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCAC  >  NC_000913/2012564‑2012830

ttATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTa                                                                                                                                  >  1:1410768/1‑139 (MQ=255)

        tataCGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCa                                                                                                                          <  1:140845/139‑1 (MQ=255)

          taCGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATg                                                                                                                        <  2:1410768/139‑1 (MQ=255)

          taCGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATg                                                                                                                        >  1:445853/1‑139 (MQ=255)

           aCGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGc                                                                                                                       <  2:160177/139‑1 (MQ=255)

              gATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGc                                                                                                                           >  1:1369057/1‑132 (MQ=255)

              gATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGc                                                                                                                           <  2:1369057/132‑1 (MQ=255)

               aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                   >  1:706971/1‑139 (MQ=255)

               aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                   <  2:706971/139‑1 (MQ=255)

               aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                   <  1:389240/139‑1 (MQ=255)

                 cGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcatca                                                                                                                 >  2:251048/1‑139 (MQ=255)

                   tGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACt                                                                                                               <  2:445853/139‑1 (MQ=255)

                         gCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGa                                                                                                         >  1:627767/1‑139 (MQ=255)

                          cAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGAt                                                                                                        <  2:838461/139‑1 (MQ=255)

                                   ttCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGcca                                                                                               <  2:10095/139‑1 (MQ=255)

                                      aGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCAGCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGccacca                                                                                            <  2:1413475/139‑1 (MQ=255)

                                       ggATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAg                                                                                           <  1:1000667/139‑1 (MQ=255)

                                              gTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATAcgcc                                                                                                   >  2:65723/1‑124 (MQ=255)

                                              gTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATAcgcc                                                                                                   <  1:65723/124‑1 (MQ=255)

                                                aTCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACg                                                                                  <  1:251048/139‑1 (MQ=255)

                                                aTCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACg                                                                                  <  1:142018/139‑1 (MQ=255)

                                                     ttttAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATAcgccgc                                                                                                 >  1:419833/1‑119 (MQ=255)

                                                     ttttAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATAcgccgc                                                                                                 <  2:419833/119‑1 (MQ=255)

                                                        tAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGaa                                                                          >  1:1226185/1‑139 (MQ=255)

                                                          aaCCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTGACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATc                                                                        <  1:900828/139‑1 (MQ=255)

                                                                              tGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                   <  2:1358712/76‑1 (MQ=255)

                                                                              tGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                   >  1:1358712/1‑76 (MQ=255)

                                                                               gCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAg                                                   <  2:792735/139‑1 (MQ=255)

                                                                                  ccatccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTa                                                                                                                                  <  1:842948/57‑1 (MQ=255)

                                                                                          gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                   <  2:532988/64‑1 (MQ=255)

                                                                                          gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                   >  1:532988/1‑64 (MQ=255)

                                                                                                cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGc                                                                                                                       >  1:765253/1‑54 (MQ=255)

                                                                                                cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGc                                                                                                                       <  2:765253/54‑1 (MQ=255)

                                                                                                cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCGCCATTTGCATGGAAACTGAGGCTTTCTGCATATCGGt                                  >  1:935492/1‑139 (MQ=255)

                                                                                                     tGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtcatca                             <  2:627767/139‑1 (MQ=255)

                                                                                                       agaTCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATGACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGATTCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATcaca                           <  2:935492/139‑1 (MQ=255)

                                                                                                               tGTAATTAACAACAGGTTACAAAACTTACACCTGc                                                                                                                           >  2:1230862/1‑35 (MQ=25)

                                                                                                               tGTAATTAACAACAGGTTACAAAACTTACACCTGc                                                                                                                           <  1:1230862/35‑1 (MQ=255)

                                                                                                                              gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGccacc                                                                                             <  2:195016/50‑1 (MQ=255)

                                                                                                                              gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGccacc                                                                                             >  1:195016/1‑50 (MQ=255)

                                                                                                                              gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCttatt                                                                                     >  2:244446/1‑58 (MQ=255)

                                                                                                                              gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCttatt                                                                                     <  1:244446/58‑1 (MQ=255)

                                                                                                                              gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACt                                                      <  2:584418/89‑1 (MQ=255)

                                                                                                                              gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACt                                                      >  1:584418/1‑89 (MQ=255)

                                                                                                                              gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTc    >  1:378895/1‑139 (MQ=255)

                                                                                                                              gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATACCGCTCACCACATCGTTTAACGCCACGCCGGGTTc    >  2:1004081/1‑139 (MQ=255)

                                                                                                                                tACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCAc  <  2:378895/139‑1 (MQ=255)

TTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCAC  >  NC_000913/2012564‑2012830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A