Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,012,700	C→T	105 (TAG→TAA)	fliE ←	flagellar basal‑body component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,012,700	0	C	T	100.0%	199.5 / NA	63	105 (TAG→TAA)	fliE	flagellar basal‑body component
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/33); total (30/33)

ATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCACCGAGA  >  NC_000913/2012579‑2012835

aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGTCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        <  1:392611/139‑1 (MQ=255)

aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        <  2:897608/139‑1 (MQ=255)

aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        <  1:1080135/139‑1 (MQ=255)

aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        >  2:1080135/1‑139 (MQ=255)

aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        >  1:767343/1‑139 (MQ=255)

aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        >  1:728751/1‑139 (MQ=255)

   gTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCAc                                                                                                                     >  1:1315421/1‑139 (MQ=255)

            aGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATCGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATa                                                                                                            >  1:1029592/1‑139 (MQ=255)

                aaCGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTaca                                                                                                                                     <  1:449840/110‑1 (MQ=255)

                aaCGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTaca                                                                                                                                     >  2:449840/1‑110 (MQ=255)

                    ttCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGcca                                                                                                    <  2:251740/139‑1 (MQ=255)

                               gTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCttatta                                                                                         <  2:728751/139‑1 (MQ=255)

                                 aTCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACg                                                                                       <  2:1157450/139‑1 (MQ=255)

                                    cATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCAc                                                                                    <  1:865047/139‑1 (MQ=255)

                                      ttttAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCt                                                                                  <  2:131340/139‑1 (MQ=255)

                                                gAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGc                                                                                                                                <  2:584471/83‑1 (MQ=255)

                                                gAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGc                                                                                                                                >  1:584471/1‑83 (MQ=255)

                                                 aaaTTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCAtt                                                                       <  2:767343/139‑1 (MQ=255)

                                                     tgagtgagTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCa                                                                   <  2:1315421/139‑1 (MQ=255)

                                                          gagtTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGc                                                                                      >  2:415584/1‑115 (MQ=255)

                                                          gagtTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGc                                                                                      <  1:415584/115‑1 (MQ=255)

                                                           agtTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGaaa                                                             >  2:966948/1‑139 (MQ=255)

                                                             tttGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACt                                                           <  2:240479/139‑1 (MQ=255)

                                                                gCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAg                                                        <  2:1029592/139‑1 (MQ=255)

                                                                     atccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCACCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAg                                                        >  2:1198074/1‑134 (MQ=255)

                                                                     atccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCACCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAg                                                        <  1:1198074/134‑1 (MQ=255)

                                                                      tccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATg                                                                                                                             >  2:1184124/1‑64 (MQ=255)

                                                                      tccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATg                                                                                                                             <  1:1184124/64‑1 (MQ=255)

                                                                      tccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGc                                                                                               >  2:885007/1‑94 (MQ=255)

                                                                      tccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGc                                                                                               <  1:885007/94‑1 (MQ=255)

                                                                          tGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACg                                                                                       >  1:31077/1‑98 (MQ=255)

                                                                          tGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACg                                                                                       <  2:31077/98‑1 (MQ=255)

                                                                          tGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCa                                              <  1:966948/139‑1 (MQ=255)

                                                                           gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        >  2:1180867/1‑64 (MQ=255)

                                                                           gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        <  1:1180867/64‑1 (MQ=255)

                                                                           gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCAtt                                                                       >  1:349151/1‑113 (MQ=255)

                                                                           gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCAtt                                                                       <  2:349151/113‑1 (MQ=255)

                                                                           gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCat                                             >  2:171992/1‑139 (MQ=255)

                                                                               cACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        <  1:1123913/60‑1 (MQ=255)

                                                                               cACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCtcat                                                                                                                        >  2:1123913/1‑60 (MQ=255)

                                                                                aCGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCata                                            >  2:634456/1‑135 (MQ=255)

                                                                                aCGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCata                                            <  1:634456/135‑1 (MQ=255)

                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCt                                                                                              <  2:776130/84‑1 (MQ=255)

                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCt                                                                                              >  1:776130/1‑84 (MQ=255)

                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGt                                       >  1:1135264/1‑139 (MQ=255)

                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGt                                       >  2:713629/1‑139 (MQ=255)

                                                                                       gagaTCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCt                                                                                              >  2:210164/1‑78 (MQ=255)

                                                                                       gagaTCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCt                                                                                              <  1:210164/78‑1 (MQ=255)

                                                                                        agaTCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATcaca                                <  1:171992/139‑1 (MQ=255)

                                                                                                    aTTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAacgcc                    >  2:1000610/1‑139 (MQ=255)

                                                                                                        acaacaGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCAtt                                                                       >  1:527579/1‑84 (MQ=255)

                                                                                                        acaacaGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCAtt                                                                       <  2:527579/84‑1 (MQ=255)

                                                                                                        acaacaGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAacgccacgc                <  1:713629/139‑1 (MQ=255)

                                                                                                         caacaGGTTACAAAACTTACACCTGCATGCTCATCACTTCTTGAt                                                                                                             >  1:819983/1‑45 (MQ=255)

                                                                                                         caacaGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGAt                                                                                                             <  2:819983/45‑1 (MQ=255)

                                                                                                         caacaGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATcccc                                                                          <  2:24692/80‑1 (MQ=255)

                                                                                                         caacaGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATcccc                                                                          >  1:24692/1‑80 (MQ=255)

                                                                                                            caGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCggg            >  2:1241033/1‑139 (MQ=255)

                                                                                                            caGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCACCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCggg            >  2:988139/1‑139 (MQ=255)

                                                                                                               gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGccac                                                                                                   <  2:1263577/49‑1 (MQ=255)

                                                                                                               gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGccac                                                                                                   >  1:1263577/1‑49 (MQ=255)

                                                                                                               gTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTc         >  2:1324497/1‑139 (MQ=255)

                                                                                                                      aaCTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCACCgaga  <  1:1000610/139‑1 (MQ=255)

ATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCACCGAGA  >  NC_000913/2012579‑2012835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A