Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I2 R1 777 59.2 2955938 92.5% 2734242 106.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,118,164 G→A R81C (CGT→TGT)  wcaK ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,118,1640GA100.0% 179.3 / NA 55R81C (CGT→TGT) wcaKcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

GCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCAT  >  NC_000913/2118046‑2118290
                                                                                                                      |                                                                                                                              
gCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGcc                                                                                                            >  1:588123/1‑139 (MQ=255)
   tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGa                                                                                                         >  1:574787/1‑139 (MQ=255)
   tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGa                                                                                                         >  1:526035/1‑139 (MQ=255)
                 cagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                           >  2:1373186/1‑139 (MQ=255)
                 cagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                           <  1:581054/139‑1 (MQ=255)
                   ggCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCt                                                                                         >  1:299753/1‑139 (MQ=255)
                    gcgcACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtg                                                                                        <  2:1039882/139‑1 (MQ=255)
                    gcgcACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtg                                                                                        <  2:588123/139‑1 (MQ=255)
                                   gAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCAtt                                                                         <  2:299753/139‑1 (MQ=255)
                                        ccTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAg                                                                    <  2:1243808/139‑1 (MQ=255)
                                        ccTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAg                                                                    <  1:1373186/139‑1 (MQ=255)
                                          tGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGa                                                                  >  1:173095/1‑139 (MQ=255)
                                                 tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCgg                                                           >  1:1134364/1‑139 (MQ=255)
                                                      atatTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCg                                                                                                          >  1:1417066/1‑87 (MQ=255)
                                                      atatTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCg                                                                                                          <  2:1417066/87‑1 (MQ=255)
                                                            cgcAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                           >  2:70874/1‑96 (MQ=255)
                                                            cgcAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                           <  1:70874/96‑1 (MQ=255)
                                                                 cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACgcgc                                                                                                             >  2:1285133/1‑73 (MQ=255)
                                                                 cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACgcgc                                                                                                             <  1:1285133/73‑1 (MQ=255)
                                                                 cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtg                                                                                        >  2:1181775/1‑94 (MQ=255)
                                                                 cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtg                                                                                        <  1:1181775/94‑1 (MQ=255)
                                                                   tGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCg                                         >  1:333415/1‑139 (MQ=255)
                                                                                 aCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGGACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAgg                           <  2:574787/139‑1 (MQ=255)
                                                                                 aCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACATCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAgg                           >  2:887944/1‑139 (MQ=255)
                                                                                   gcgTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg                                                                                   >  1:288447/1‑81 (MQ=255)
                                                                                   gcgTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg                                                                                   <  2:288447/81‑1 (MQ=255)
                                                                                   gcgTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaa                         >  1:916316/1‑139 (MQ=255)
                                                                                     gTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaaga                       <  1:887944/139‑1 (MQ=255)
                                                                                           gCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                   <  1:709865/121‑1 (MQ=255)
                                                                                           gCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                   >  2:709865/1‑121 (MQ=255)
                                                                                               tACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                           >  1:1138402/1‑61 (MQ=255)
                                                                                               tACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                           <  2:1138402/61‑1 (MQ=255)
                                                                                                  cTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACAGACCGGATAg          <  2:805373/139‑1 (MQ=255)
                                                                                                   tGATGCTGGTAGCGGCGACAGAGGACCTTTTTAAcg                                                                                                                <  2:986768/36‑1 (MQ=255)
                                                                                                   tGATGCTGGTAGCCGCGACAGAGGACTTTTTTAAcg                                                                                                                >  1:986768/1‑36 (MQ=255)
                                                                                                    gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa                                                                           <  2:1404795/72‑1 (MQ=255)
                                                                                                    gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa                                                                           >  1:1404795/1‑72 (MQ=255)
                                                                                                    gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaaga                       <  2:1135245/124‑1 (MQ=255)
                                                                                                    gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaaga                       >  1:1135245/1‑124 (MQ=255)
                                                                                                    gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGa                  >  1:928239/1‑129 (MQ=255)
                                                                                                    gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGa                  <  2:928239/129‑1 (MQ=255)
                                                                                                     ctGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaa                         >  1:918217/2‑121 (MQ=255)
                                                                                                     aTGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGAGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaa                         <  2:918217/121‑1 (MQ=255)
                                                                                                      tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc      <  2:375257/139‑1 (MQ=255)
                                                                                                         tGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                   >  2:1434101/1‑107 (MQ=255)
                                                                                                         tGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                   <  1:1434101/107‑1 (MQ=255)
                                                                                                          ggTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                   >  1:636840/1‑106 (MQ=255)
                                                                                                          ggTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                   <  2:636840/106‑1 (MQ=255)
                                                                                                          ggTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcat  <  2:1134364/139‑1 (MQ=255)
                                                                                                            tAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                   <  1:1252414/104‑1 (MQ=255)
                                                                                                            tAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                   >  2:1252414/1‑104 (MQ=255)
                                                                                                            tAGCGGCGACAGAGGACTTTTTTAACGCG‑CCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAg                                                              >  2:189444/1‑76 (MQ=255)
                                                                                                            tAGCGGCGACAGAGGACTTTTTTAACGCG‑CCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAg                                                              <  1:189444/76‑1 (MQ=255)
                                                                                                               cggcgACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                             <  2:485253/43‑1 (MQ=255)
                                                                                                               cggcgACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                             >  1:485253/1‑43 (MQ=255)
                                                                                                                      |                                                                                                                              
GCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCAT  >  NC_000913/2118046‑2118290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A