Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,118,164 |
G→A |
R81C (CGT→TGT) |
wcaK ← |
colanic acid biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,118,164 | 0 | G | A | 100.0%
| 159.5
/ NA
| 49 | R81C (CGT→TGT) | wcaK | colanic acid biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/25); total (24/25) |
ACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCAT > NC_000913/2118034‑2118290
|
aCCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACtt > 1:622075/1‑139 (MQ=255)
aTAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTaa < 2:653498/139‑1 (MQ=255)
tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAAcgcg > 1:854901/1‑139 (MQ=255)
tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGa > 1:796605/1‑139 (MQ=255)
cTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcc < 2:801979/139‑1 (MQ=255)
cagcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccgcc < 2:268007/139‑1 (MQ=255)
cagcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccgcc < 2:796605/139‑1 (MQ=255)
ggCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCt > 1:618202/1‑139 (MQ=255)
cACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACGTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccgcc > 2:1237962/1‑130 (MQ=255)
cACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccgcc < 1:1237962/130‑1 (MQ=255)
cACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttg > 1:615784/1‑139 (MQ=255)
gAAGTCGGTGAATCCCTGGGCGATGGCGATATTGTGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgt < 2:973823/139‑1 (MQ=255)
tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAAcg > 1:471920/1‑86 (MQ=255)
tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAAcg < 2:471920/86‑1 (MQ=255)
tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg > 1:317226/1‑107 (MQ=255)
tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg < 2:317226/107‑1 (MQ=255)
tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCgg > 1:471907/1‑139 (MQ=255)
ggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg < 1:857055/114‑1 (MQ=255)
ggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg > 2:857055/1‑114 (MQ=255)
cAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTAcc > 2:952400/1‑139 (MQ=255)
cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcc > 1:131367/1‑84 (MQ=255)
cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcc < 2:131367/84‑1 (MQ=255)
cAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa > 2:684524/1‑119 (MQ=255)
cAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa < 1:684524/119‑1 (MQ=255)
tgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa < 2:666553/116‑1 (MQ=255)
tgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa > 1:666553/1‑116 (MQ=255)
tgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag < 2:622075/139‑1 (MQ=255)
tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTa < 1:1062407/57‑1 (MQ=255)
tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTa > 2:1062407/1‑57 (MQ=255)
gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccgcc > 2:985652/1‑75 (MQ=255)
gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccgcc < 1:985652/75‑1 (MQ=255)
aCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa < 1:657088/91‑1 (MQ=255)
aCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCCACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa > 2:657088/1‑91 (MQ=255)
cgTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTATTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaag > 1:137283/1‑139 (MQ=255)
gagaGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTATTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAAc < 2:137283/139‑1 (MQ=255)
agagCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACt < 2:597384/139‑1 (MQ=255)
ccTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATa < 2:615784/139‑1 (MQ=255)
gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa < 2:101161/89‑1 (MQ=255)
gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa > 1:101161/1‑89 (MQ=255)
aTGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg < 2:410922/55‑1 (MQ=255)
aTGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg > 1:410922/1‑55 (MQ=255)
tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc < 1:1122715/139‑1 (MQ=255)
ggTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcat > 2:1141421/1‑139 (MQ=255)
gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg < 2:636799/47‑1 (MQ=255)
gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg > 1:636799/1‑47 (MQ=255)
gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGc < 2:1120724/70‑1 (MQ=255)
gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGc > 1:1120724/1‑70 (MQ=255)
gcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGc > 1:976403/1‑64 (MQ=255)
gcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGc < 2:976403/64‑1 (MQ=255)
|
ACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCAT > NC_000913/2118034‑2118290
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
