Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 760 58.2 3022526 90.8% 2744453 102.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,118,164 G→A R81C (CGT→TGT)  wcaK ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,118,1640GA100.0% 179.2 / NA 55R81C (CGT→TGT) wcaKcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/23);  total (32/23)

AATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATCCA  >  NC_000913/2118042‑2118298
                                                                                                                          |                                                                                                                                      
aaTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAAcg                                                                                                                        <  2:911769/139‑1 (MQ=255)
aaTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAAcg                                                                                                                        <  1:127917/139‑1 (MQ=255)
       tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACagag                                                                                                                                     <  2:1506766/119‑1 (MQ=255)
       tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACagag                                                                                                                                     >  1:1506766/1‑119 (MQ=255)
       tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGa                                                                                                                 >  2:1197329/1‑139 (MQ=255)
                   agcagGCGCACGAAGTCGGTGAATTCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                     <  1:1046049/139‑1 (MQ=255)
                   agcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                     <  2:1127770/139‑1 (MQ=255)
                       ggCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCt                                                                                                 >  1:1214065/1‑139 (MQ=255)
                                     gTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa                                                                                   <  2:918289/139‑1 (MQ=255)
                                          tCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAAcgcg                                                                                                                      >  2:288325/1‑99 (MQ=255)
                                          tCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAAcgcg                                                                                                                      <  1:288325/99‑1 (MQ=255)
                                           cccTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCa                                                                             <  1:910466/139‑1 (MQ=255)
                                                                     cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                   <  1:1074540/91‑1 (MQ=255)
                                                                     cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                   >  2:1074540/1‑91 (MQ=255)
                                                                     cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGcc                                                             >  1:1110361/1‑129 (MQ=255)
                                                                     cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGcc                                                             <  2:1110361/129‑1 (MQ=255)
                                                                        gCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCgg                                                >  2:1437764/1‑139 (MQ=255)
                                                                               tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagca                                         >  1:400372/1‑139 (MQ=255)
                                                                               tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagca                                         >  2:220080/1‑139 (MQ=255)
                                                                                  gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                           <  1:26521/134‑1 (MQ=255)
                                                                                  gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                           >  2:26521/1‑134 (MQ=255)
                                                                                  gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACACGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa                                                                  >  1:378242/1‑111 (MQ=255)
                                                                                  gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACACGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa                                                                  <  2:378242/111‑1 (MQ=255)
                                                                                   tGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCa                                     >  2:858891/1‑139 (MQ=255)
                                                                                       gcgTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaa                                 >  2:725843/1‑139 (MQ=255)
                                                                                        cgTGAGAGCGATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                        <  1:928224/131‑1 (MQ=255)
                                                                                        cgTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACATTTTTAACGAGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                        >  2:928224/1‑131 (MQ=255)
                                                                                         gTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCa                                                           >  1:1273078/1‑111 (MQ=255)
                                                                                         gTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCa                                                           <  2:1273078/111‑1 (MQ=255)
                                                                                          tGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaagaa                              >  2:943854/1‑139 (MQ=255)
                                                                                           gagaGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAAc                             <  1:725843/139‑1 (MQ=255)
                                                                                             gagCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCACCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTg                           >  1:673499/1‑139 (MQ=255)
                                                                                                   tACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGa                     >  2:1059698/1‑139 (MQ=255)
                                                                                                   tACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGa                     >  2:543085/1‑139 (MQ=255)
                                                                                                    aCCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGAt                    <  1:220080/139‑1 (MQ=255)
                                                                                                        gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGAt                                                                 <  1:722741/90‑1 (MQ=255)
                                                                                                        gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGAt                                                                 >  2:722741/1‑90 (MQ=255)
                                                                                                        gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCg                >  1:1406208/1‑139 (MQ=255)
                                                                                                        gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCg                >  2:722186/1‑139 (MQ=255)
                                                                                                          tGCTGGTAGGGGGGACAGAGGCCTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc              <  2:810291/139‑1 (MQ=255)
                                                                                                          tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                     >  2:1272746/1‑52 (MQ=255)
                                                                                                          tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                     >  2:84084/1‑52 (MQ=255)
                                                                                                          tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                     <  1:1272746/52‑1 (MQ=255)
                                                                                                          tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                     <  1:84084/52‑1 (MQ=255)
                                                                                                          tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc              >  2:490492/1‑139 (MQ=255)
                                                                                                          tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc              <  1:722186/139‑1 (MQ=255)
                                                                                                          tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc              >  2:308169/1‑139 (MQ=255)
                                                                                                               gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                     >  1:1278498/1‑47 (MQ=255)
                                                                                                               gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                     <  2:1278498/47‑1 (MQ=255)
                                                                                                               gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGcc                                                             <  1:952881/87‑1 (MQ=255)
                                                                                                               gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGcc                                                             >  2:952881/1‑87 (MQ=255)
                                                                                                               gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcatc         >  2:406166/1‑139 (MQ=255)
                                                                                                                   cggcgACAGAGGACTTTTTTAACGCGCCTGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACAt     >  2:1268363/1‑139 (MQ=255)
                                                                                                                    ggcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc              <  2:480008/129‑1 (MQ=255)
                                                                                                                    ggcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc              >  1:480008/1‑129 (MQ=255)
                                                                                                                     acgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                           <  1:311288/98‑1 (MQ=255)
                                                                                                                     acgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                           >  2:311288/2‑99 (MQ=255)
                                                                                                                      cgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATCCa  >  2:937932/1‑139 (MQ=255)
                                                                                                                          |                                                                                                                                      
AATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATCCA  >  NC_000913/2118042‑2118298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A