Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	542,530	A→G	T215A (ACG→GCG)	glxK →	glycerate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	542,530	0	A	G	100.0%	176.2 / NA	49	T215A (ACG→GCG)	glxK	glycerate kinase II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/22); total (27/22)

CGCGTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCG  >  NC_000913/542395‑542655

cgcgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAgcgc                                                                                                                            >  1:748012/1‑139 (MQ=255)

  cgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCg                                                                                                                          >  1:835636/1‑139 (MQ=255)

  cgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCg                                                                                                                          >  2:1088377/1‑139 (MQ=255)

  cgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGAGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCg                                                                                                                          >  2:1035183/1‑139 (MQ=255)

                     gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTg                                                                                                       >  2:51799/1‑139 (MQ=255)

                     gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCC‑CTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCga                                                                                                               >  2:1418399/1‑130 (MQ=255)

                     gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCC‑CTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCga                                                                                                               <  1:1418399/130‑1 (MQ=255)

                                 ccGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                           <  1:51799/139‑1 (MQ=255)

                                          gCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACg                                                                                  >  1:1316316/1‑139 (MQ=255)

                                              aTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              <  1:1035183/139‑1 (MQ=255)

                                              aTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              <  2:748012/139‑1 (MQ=255)

                                              aTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              <  1:1088377/139‑1 (MQ=255)

                                                          aaGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTacaaca                                                                  >  1:480513/1‑139 (MQ=255)

                                                            gTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGc                                                                <  1:1288756/139‑1 (MQ=255)

                                                                cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              >  1:1504675/1‑121 (MQ=255)

                                                                cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              <  2:1504675/121‑1 (MQ=255)

                                                                cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                            <  2:480513/139‑1 (MQ=255)

                                                                cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                            >  2:844850/1‑139 (MQ=255)

                                                                  tgtgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCg                                                                                                                          <  1:1453376/75‑1 (MQ=255)

                                                                  tgtgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCg                                                                                                                          >  2:1453376/1‑75 (MQ=255)

                                                                    tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCaa                                                                                         >  2:1288782/1‑106 (MQ=255)

                                                                    tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCaa                                                                                         <  1:1288782/106‑1 (MQ=255)

                                                                                     tGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                                       >  2:181873/1‑139 (MQ=255)

                                                                                      ggTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCgaag                                                                                                             <  2:1042912/68‑1 (MQ=255)

                                                                                      ggTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCgaag                                                                                                             >  1:1042912/1‑68 (MQ=255)

                                                                                          ggggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCga                                                                                                               >  1:1293767/1‑62 (MQ=255)

                                                                                          ggggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCga                                                                                                               <  2:1293767/62‑1 (MQ=255)

                                                                                           gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcggg                                 >  1:631630/1‑139 (MQ=255)

                                                                                            ggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAgggg                                                                                               <  1:874215/76‑1 (MQ=255)

                                                                                            ggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAgggg                                                                                               >  2:874215/1‑76 (MQ=255)

                                                                                            ggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggc                                >  2:1379501/1‑139 (MQ=255)

                                                                                              cACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                              >  2:670547/1‑139 (MQ=255)

                                                                                               aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggc                             >  2:1208072/1‑139 (MQ=255)

                                                                                                 gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcgg                           >  1:760396/1‑139 (MQ=255)

                                                                                                 gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcgg                           >  2:952438/1‑139 (MQ=255)

                                                                                                 gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAAGGGCAGCGCCAGAGATGGTCGAAGAACTTGAACAGAGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcgg                           >  1:695268/1‑139 (MQ=255)

                                                                                                  cggcgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                          >  1:366895/1‑139 (MQ=255)

                                                                                                  cggcgcggcgGCGGTGTTTGGCCCACAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              <  2:1505332/87‑1 (MQ=255)

                                                                                                  cggcgcggcgGCGGTGTTTGGCCCACAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              >  1:1505332/1‑87 (MQ=255)

                                                                                                     cgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCt                       <  1:670547/139‑1 (MQ=255)

                                                                                                       cggcggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgc                     >  1:431450/1‑139 (MQ=255)

                                                                                                       cggcggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgc                     <  2:631630/139‑1 (MQ=255)

                                                                                                         gcggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTccgg                   <  2:1537842/139‑1 (MQ=255)

                                                                                                             cggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              <  1:489704/76‑1 (MQ=255)

                                                                                                             cggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                              >  2:489704/1‑76 (MQ=255)

                                                                                                                tgtTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt            <  2:835636/139‑1 (MQ=255)

                                                                                                                tgtTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt            <  1:844850/139‑1 (MQ=255)

                                                                                                                          gCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcg  <  2:760396/139‑1 (MQ=255)

                                                                                                                            aaaaaGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTa                                                                                    >  2:547538/1‑55 (MQ=255)

                                                                                                                            aaaaaGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTa                                                                                    <  1:547538/55‑1 (MQ=255)

CGCGTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCG  >  NC_000913/542395‑542655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A