Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	542,530	A→G	T215A (ACG→GCG)	glxK →	glycerate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	542,530	0	A	G	100.0%	153.9 / NA	43	T215A (ACG→GCG)	glxK	glycerate kinase II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/19); total (24/19)

ATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTA  >  NC_000913/542421‑542644

aTGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAg                                                                                       >  1:149607/1‑139 (MQ=255)

 tGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                      >  1:1300995/1‑139 (MQ=255)

  gCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAggg                                                                                     >  2:973426/1‑139 (MQ=255)

  gCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAggg                                                                                     >  1:193304/1‑139 (MQ=255)

     aTCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCt                                                                                  >  2:462529/1‑139 (MQ=255)

       ccGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                <  1:462529/139‑1 (MQ=255)

         gtgTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCaa                                                                              >  2:234568/3‑139 (MQ=255)

                     ttGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGACAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                <  2:852309/125‑1 (MQ=255)

                     ttGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGACAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                >  1:852309/1‑125 (MQ=255)

                                aaGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTacaaca                                                       <  1:946128/139‑1 (MQ=255)

                                  gTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGTGCCGgaga                                                                                                           >  2:961344/1‑85 (MQ=255)

                                  gTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGgaga                                                                                                           <  1:961344/85‑1 (MQ=255)

                                      cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                 >  2:187803/1‑139 (MQ=255)

                                        tgtgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                               >  1:58386/1‑139 (MQ=255)

                                          tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaa                                             >  2:789282/1‑139 (MQ=255)

                                          tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaa                                             >  2:1252914/1‑139 (MQ=255)

                                               ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCgaag                                                                                                  >  1:1303328/1‑81 (MQ=255)

                                               ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCgaag                                                                                                  <  2:1303328/81‑1 (MQ=255)

                                                tGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAt                                       >  2:225243/1‑139 (MQ=255)

                                                 gATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATg                                      <  1:309996/139‑1 (MQ=255)

                                                      cccGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCaaa                                                  >  1:55634/1‑122 (MQ=255)

                                                      cccGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCaaa                                                  <  2:55634/122‑1 (MQ=255)

                                                          cTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGt                                     <  2:706707/131‑1 (MQ=255)

                                                          cTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGt                                     >  1:706707/1‑131 (MQ=255)

                                                          cTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                             <  1:973426/139‑1 (MQ=255)

                                                           tGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                            >  1:97783/1‑139 (MQ=255)

                                                                 gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                 <  2:756093/112‑1 (MQ=255)

                                                                 gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                 >  1:756093/1‑112 (MQ=255)

                                                                       gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                >  1:488603/1‑75 (MQ=255)

                                                                       gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                <  2:488603/75‑1 (MQ=255)

                                                                       gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGccc                                                                    >  1:93426/1‑87 (MQ=255)

                                                                       gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGccc                                                                    <  2:93426/87‑1 (MQ=255)

                                                                            gcggcggcgGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                      >  2:795151/1‑64 (MQ=255)

                                                                            gcggcggcgGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                      <  1:795151/64‑1 (MQ=255)

                                                                             cggcggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAAc                                                                                         <  2:970808/60‑1 (MQ=255)

                                                                             cggcggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAAc                                                                                         >  1:970808/1‑60 (MQ=255)

                                                                                cggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg       <  1:187803/139‑1 (MQ=255)

                                                                                cggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg       <  2:58386/139‑1 (MQ=255)

                                                                                 ggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc      <  2:193304/139‑1 (MQ=255)

                                                                                    ggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGTGGCGGCGCTGCGGGCGGt   <  1:789282/139‑1 (MQ=255)

                                                                                     gtgtTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTa  >  2:56916/1‑139 (MQ=255)

                                                                                                  aaaaaGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATg                                      >  1:496231/1‑90 (MQ=255)

                                                                                                  aaaaaGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATg                                      <  2:496231/90‑1 (MQ=255)

ATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTA  >  NC_000913/542421‑542644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A