Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	942,735	A→G	M593V (ATG→GTG)	dmsA →	dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	942,735	0	A	G	100.0%	181.1 / NA	52	M593V (ATG→GTG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/23); total (29/23)

CTTACGTGATTTTCAACGATCAGGTGATTAAACCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGATGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCTTCCGTGAAGATC  >  NC_000913/942601‑942870

cTTACGTGATTTTCAACGATCAGGTGATTAAACCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCg                                                                                                                                     <  1:1349335/139‑1 (MQ=255)

  tACGTGATTTTCAACGATCAGGTGATTAAACCCCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGc                                                                                                                                   <  2:1459424/139‑1 (MQ=255)

                            tAAACCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGACGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCg                                                                                                         >  2:408174/1‑139 (MQ=255)

                               aCCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGAtt                                                                                                      >  1:1122426/1‑139 (MQ=255)

                                 cgcgCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTcc                                                                                                    <  1:1599753/139‑1 (MQ=255)

                                  gcgcTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCt                                                                                                   >  1:275502/1‑139 (MQ=255)

                                  gcgcTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCt                                                                                                   <  1:408174/139‑1 (MQ=255)

                                          aaTGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGcc                                                                                           <  2:443695/139‑1 (MQ=255)

                                            tGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCaa                                                                                         <  1:141019/139‑1 (MQ=255)

                                                aGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGt                                                                                                                             >  2:626566/1‑99 (MQ=255)

                                                aGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGt                                                                                                                             <  1:626566/99‑1 (MQ=255)

                                                    cATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAAc                                                                                        >  2:902555/1‑132 (MQ=255)

                                                    cATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAAc                                                                                        <  1:902555/132‑1 (MQ=255)

                                                        tATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGt                                                                             >  1:959510/1‑139 (MQ=255)

                                                           gAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTcc                                                                          <  2:959510/139‑1 (MQ=255)

                                                              aTGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCa                                                                       <  2:577392/139‑1 (MQ=255)

                                                                gACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGa                                                                                                        >  2:152212/1‑104 (MQ=255)

                                                                gACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGa                                                                                                        <  1:152212/104‑1 (MQ=255)

                                                                 aCCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTg                                                                                   <  1:240767/124‑1 (MQ=255)

                                                                 aCCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTg                                                                                   >  2:240767/1‑124 (MQ=255)

                                                                 aCCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGc                                                                    >  1:699157/1‑139 (MQ=255)

                                                                 aCCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGACGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGc                                                                    >  1:1016921/1‑139 (MQ=255)

                                                                  ccAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCa                                                                   >  2:149297/1‑139 (MQ=255)

                                                                  ccAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTACGCAAGCa                                                                   >  1:979878/1‑139 (MQ=255)

                                                                    aGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCg                                                                         <  1:686642/131‑1 (MQ=255)

                                                                    aGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCg                                                                         >  2:686642/1‑131 (MQ=255)

                                                                      cGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAgggg                                                               >  2:352555/1‑139 (MQ=255)

                                                                         aCTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATc                                                            <  2:1122426/139‑1 (MQ=255)

                                                                           tGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCtt                                                          >  2:655566/1‑139 (MQ=255)

                                                                             gCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTa                                                        >  2:673886/1‑139 (MQ=255)

                                                                                    gTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAgcg                                                 <  2:275502/139‑1 (MQ=255)

                                                                                      cTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGcgcg                                               >  2:212163/1‑139 (MQ=255)

                                                                                                aGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAgg                                     >  1:1143556/1‑139 (MQ=255)

                                                                                                     cAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATc                                >  1:221976/1‑139 (MQ=255)

                                                                                                        tttACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAggg                                                                >  1:853974/1‑104 (MQ=255)

                                                                                                        tttACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAggg                                                                <  2:853974/104‑1 (MQ=255)

                                                                                                          tACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAg                                                                     >  2:1508026/1‑97 (MQ=255)

                                                                                                          tACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAg                                                                     <  1:1508026/97‑1 (MQ=255)

                                                                                                           aCTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTg                          <  2:699157/139‑1 (MQ=255)

                                                                                                            cTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGc                         <  1:673886/139‑1 (MQ=255)

                                                                                                               aaGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTta                      >  2:655835/1‑139 (MQ=255)

                                                                                                                  gCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGc                                                                                                          >  1:353277/1‑52 (MQ=255)

                                                                                                                  gCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGc                                                                                                          <  2:353277/52‑1 (MQ=255)

                                                                                                                  gCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAgcgc                                                >  2:1019826/1‑110 (MQ=255)

                                                                                                                  gCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAgcgc                                                <  1:1019826/110‑1 (MQ=255)

                                                                                                                      tACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGcc               >  1:1114230/1‑139 (MQ=255)

                                                                                                                      tACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCACAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGcc               >  2:574040/1‑139 (MQ=255)

                                                                                                                       aCCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCt              <  1:655835/139‑1 (MQ=255)

                                                                                                                                   tGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACg                             <  1:286749/112‑1 (MQ=255)

                                                                                                                                   tGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACg                             >  2:286749/1‑112 (MQ=255)

                                                                                                                                   tGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCTTCCGTGAAGATc  >  2:442991/1‑139 (MQ=255)

                                                                                                                                   tGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCG‑TTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAgcg                                                 >  2:605167/1‑91 (MQ=255)

CTTACGTGATTTTCAACGATCAGGTGATTAAACCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGATGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCTTCCGTGAAGATC  >  NC_000913/942601‑942870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A