Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
947,123 |
T→C |
intergenic (+104/‑106) |
ycaD → / → ycaM |
putative MFS transporter, inner membrane protein/putative transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 947,123 | 0 | T | C | 100.0%
| 105.2
/ NA
| 30 | intergenic (+104/‑106) | ycaD/ycaM | putative MFS transporter, inner membrane protein/putative transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/15); total (15/15) |
GTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGTGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGG > NC_000913/946988‑947247
|
gTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgca > 2:894541/1‑139 (MQ=255)
gAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag > 2:742028/1‑130 (MQ=255)
gAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag < 1:742028/130‑1 (MQ=255)
aCCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTta > 2:873497/1‑134 (MQ=255)
aCCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTta < 1:873497/134‑1 (MQ=255)
aCGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGc < 1:894541/139‑1 (MQ=255)
tgAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCcgcg > 2:562004/1‑139 (MQ=255)
aaCTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCa < 1:562004/139‑1 (MQ=255)
aCTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCg < 1:340105/105‑1 (MQ=255)
aCTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCg > 2:340105/1‑105 (MQ=255)
gTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag < 1:1140308/80‑1 (MQ=255)
gTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag > 2:1140308/1‑80 (MQ=255)
gTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCcgc > 2:922352/1‑128 (MQ=255)
gTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCcgc < 1:922352/128‑1 (MQ=255)
tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcagc < 2:955177/70‑1 (MQ=255)
tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcagc > 1:955177/1‑70 (MQ=255)
tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag < 2:393578/69‑1 (MQ=255)
tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag > 1:393578/1‑69 (MQ=255)
tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATc < 1:935844/125‑1 (MQ=255)
tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATc > 2:935844/1‑125 (MQ=255)
cTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGAt < 2:1286241/116‑1 (MQ=255)
cTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGAt > 1:1286241/1‑116 (MQ=255)
ttGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGAt < 2:316898/139‑1 (MQ=255)
gCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCg < 2:358639/73‑1 (MQ=255)
gCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCg > 1:358639/1‑73 (MQ=255)
tttATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGt > 1:1093656/1‑139 (MQ=255)
aTTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTa < 2:1093656/139‑1 (MQ=255)
aaTTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGAt < 2:487970/126‑1 (MQ=255)
aaTTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGAt > 1:487970/1‑126 (MQ=255)
ttAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAgg > 1:975263/1‑139 (MQ=255)
|
GTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGTGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGG > NC_000913/946988‑947247
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
