Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	967,118	A→G	Q166Q (CAA→CAG)	msbA →	lipid ABC transporter permease/ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	967,118	0	A	G	100.0%	226.9 / NA	65	Q166Q (CAA→CAG)	msbA	lipid ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (34/31); total (34/31)

GTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTG  >  NC_000913/966995‑967253

gtCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCg                                                                                                                                    >  1:785080/1‑130 (MQ=255)

gtCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCg                                                                                                                                    <  2:785080/130‑1 (MQ=255)

gtCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTg                                                                                                                           >  1:702953/1‑139 (MQ=255)

        ttACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCt                                                                                                                   >  2:1556110/1‑139 (MQ=255)

        ttACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCt                                                                                                                   >  1:536739/1‑139 (MQ=255)

          aCCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGAt                                                                                                                                  >  2:557620/1‑122 (MQ=255)

          aCCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGAt                                                                                                                                  <  1:557620/122‑1 (MQ=255)

             tACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCAc                                                                                                              <  1:1294220/139‑1 (MQ=255)

               cGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGTCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCg                                                                                                            >  1:173148/1‑139 (MQ=255)

                                cttcttcttcttCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                           <  2:1588811/139‑1 (MQ=255)

                                      cttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGt                                                                                     >  2:1109084/1‑139 (MQ=255)

                                               gcgcACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGaa                                                                            >  1:908893/1‑139 (MQ=255)

                                               gcgcACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGaa                                                                            <  2:536739/139‑1 (MQ=255)

                                                  cACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCg                                                                         >  1:1090314/1‑139 (MQ=255)

                                                    cTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCg                                                                                                                                    >  1:558942/1‑78 (MQ=255)

                                                    cTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCg                                                                                                                                    <  2:558942/78‑1 (MQ=255)

                                                            tgttgtGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCGGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                               <  2:1090314/139‑1 (MQ=255)

                                                              ttgtGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACAt                                                             >  1:147396/1‑139 (MQ=255)

                                                               tgtgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATc                                                            >  1:1039668/1‑139 (MQ=255)

                                                                 tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACa                                                              <  1:288154/135‑1 (MQ=255)

                                                                 tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACa                                                              >  2:288154/1‑135 (MQ=255)

                                                                   cGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCg                                                                                                            >  1:1515604/1‑87 (MQ=255)

                                                                   cGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCg                                                                                                            <  2:1515604/87‑1 (MQ=255)

                                                                     tGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCAttt                                                                                                                             >  2:784520/1‑68 (MQ=255)

                                                                     tGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCAttt                                                                                                                             <  1:784520/68‑1 (MQ=255)

                                                                     tGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑aaa                                                     >  2:1186734/1‑139 (MQ=255)

                                                                        aGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAAc                                                  <  2:702953/139‑1 (MQ=255)

                                                                               tCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACGTGCAGa                                           >  1:1434960/1‑139 (MQ=255)

                                                                                cGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGaa                                          <  2:894929/139‑1 (MQ=255)

                                                                                cGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGaa                                          <  1:1186734/139‑1 (MQ=255)

                                                                                 gATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAAACATGCAGaa                                          <  2:147396/139‑1 (MQ=255)

                                                                                     atcGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCa                                     <  2:1039668/139‑1 (MQ=255)

                                                                                     atcGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCa                                     <  2:908893/139‑1 (MQ=255)

                                                                                              ttCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                               <  2:271836/105‑1 (MQ=255)

                                                                                              ttCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                               >  1:271836/1‑105 (MQ=255)

                                                                                                 atcatGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGc                               <  1:1278556/133‑1 (MQ=255)

                                                                                                 atcatGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGc                               >  2:1278556/1‑133 (MQ=255)

                                                                                                   catGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAg                             >  2:242148/1‑133 (MQ=255)

                                                                                                   catGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAg                             <  1:242148/133‑1 (MQ=255)

                                                                                                   catGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGacc                       >  2:991893/1‑139 (MQ=255)

                                                                                                     tgatgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACGTGCAGAACACCATGGGGCAGGTGaccac                     <  2:1434960/139‑1 (MQ=255)

                                                                                                          ttCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAgcg                >  1:85369/1‑139 (MQ=255)

                                                                                                           tCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGc                                                                                                                >  1:783296/1‑43 (MQ=255)

                                                                                                           tCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGc                                                                                                                <  2:783296/43‑1 (MQ=255)

                                                                                                           tCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAgcgc               <  1:991893/139‑1 (MQ=255)

                                                                                                             tATTACAGTTAGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGc                               <  2:1290707/121‑1 (MQ=255)

                                                                                                             tATTACAGTTAGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGc                               >  1:1290707/1‑121 (MQ=255)

                                                                                                               ttACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGaa           <  1:1109084/139‑1 (MQ=255)

                                                                                                               ttACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGaa           >  1:501279/1‑139 (MQ=255)

                                                                                                                tACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAg                             <  1:890011/120‑1 (MQ=255)

                                                                                                                tACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAg                             >  2:890011/1‑120 (MQ=255)

                                                                                                                    gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGtt                                                                                    >  1:892736/1‑62 (MQ=255)

                                                                                                                    gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGtt                                                                                    <  2:892736/62‑1 (MQ=255)

                                                                                                                    gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGaa                                          <  2:993366/103‑1 (MQ=255)

                                                                                                                    gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTATTTCGATTGGGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGaa                                          >  1:993366/1‑103 (MQ=255)

                                                                                                                     ttGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATg     >  2:854019/1‑139 (MQ=255)

                                                                                                                       ggCAGCTGTCGATCATTTTGATTGTGTTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAg                                            >  1:139743/1‑98 (MQ=255)

                                                                                                                       ggCAGCTGTCGATCATTTTGATTGTGTTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAg                                            <  2:139743/98‑1 (MQ=255)

                                                                                                                       ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGaa                                          >  1:385769/1‑100 (MQ=255)

                                                                                                                       ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGaa                                          <  2:385769/100‑1 (MQ=255)

                                                                                                                       ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGaccac                     >  2:76748/1‑121 (MQ=255)

                                                                                                                       ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGaccac                     <  1:76748/121‑1 (MQ=255)

                                                                                                                       ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCt   >  1:335825/1‑139 (MQ=255)

                                                                                                                       ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCt   >  1:439929/1‑139 (MQ=255)

                                                                                                                        gCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTg  <  1:1556110/139‑1 (MQ=255)

GTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGT‑AAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTG  >  NC_000913/966995‑967253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A