Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I2 R1 760 67.6 3323148 92.7% 3080558 109.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 967,118 A→G Q166Q (CAA→CAG)  msbA → lipid ABC transporter permease/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913967,1180AG100.0% 199.3 / NA 55Q166Q (CAA→CAG) msbAlipid ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/25);  total (30/25)

TATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCT  >  NC_000913/967001‑967252
                                                                                                                     |                                                                                                                                      
tATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATtgtg                                                                                                                   >  2:710844/1‑139 (MQ=255)
tATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATtgtg                                                                                                                   <  2:1233452/139‑1 (MQ=255)
  ttACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCt                                                                                                                 >  2:950052/1‑139 (MQ=255)
  ttACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCt                                                                                                                 >  1:518826/1‑139 (MQ=255)
     ccTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGc                                                                                                              >  1:1052938/1‑139 (MQ=255)
      cTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCa                                                                                                             <  1:950052/139‑1 (MQ=255)
         cGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCg                                                                                                          >  2:1259940/1‑139 (MQ=255)
         cGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCg                                                                                                          >  1:399540/1‑139 (MQ=255)
          gATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGa                                                                                                         >  2:1659388/1‑139 (MQ=255)
          gATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGa                                                                                                         >  2:1083593/1‑139 (MQ=255)
           aTTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGAt                                                                                                        <  2:444272/139‑1 (MQ=255)
             tCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCAt                                                                                                                             >  2:1209892/1‑116 (MQ=255)
             tCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCAt                                                                                                                             <  1:1209892/116‑1 (MQ=255)
             tCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTg                                                                                                      >  1:142923/1‑139 (MQ=255)
               cGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGtt                                                                                                    >  1:635033/1‑139 (MQ=255)
                gAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGttt                                                                                                   <  2:142923/139‑1 (MQ=255)
                             cttcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                                      <  1:1083593/139‑1 (MQ=255)
                               tcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                    <  1:1259940/139‑1 (MQ=255)
                               tcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                    <  2:399540/139‑1 (MQ=255)
                               tcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                    <  1:1659388/139‑1 (MQ=255)
                                                   tACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGc                                                                <  1:710844/139‑1 (MQ=255)
                                                      tgttgtGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                             <  2:518826/139‑1 (MQ=255)
                                                        ttgtGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACAt                                                           >  1:977897/1‑139 (MQ=255)
                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGAt                                                                                                        >  1:197299/1‑91 (MQ=255)
                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGAt                                                                                                        <  2:197299/91‑1 (MQ=255)
                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAg                                                        >  2:120657/1‑139 (MQ=255)
                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAg                                                        >  1:1384151/1‑139 (MQ=255)
                                                             cGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTa                                                      >  1:1084522/1‑139 (MQ=255)
                                                                gAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTaaaa                                                   >  1:379909/1‑139 (MQ=255)
                                                                     tGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                                      <  1:1350033/99‑1 (MQ=255)
                                                                     tGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                                      >  2:1350033/1‑99 (MQ=255)
                                                                       cGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                                      >  2:702581/1‑97 (MQ=255)
                                                                       cGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                                      <  1:702581/97‑1 (MQ=255)
                                                                          cGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                         <  2:1601298/139‑1 (MQ=255)
                                                                                    ccTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGa                                                                                                                        >  1:1560172/1‑50 (MQ=255)
                                                                                    ccTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGa                                                                                                                        <  2:1560172/50‑1 (MQ=255)
                                                                                     cTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                              <  2:379909/139‑1 (MQ=255)
                                                                                                  tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                         >  2:715202/1‑67 (MQ=255)
                                                                                                  tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                         <  1:715202/67‑1 (MQ=255)
                                                                                                  tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                                      >  1:1272802/1‑70 (MQ=255)
                                                                                                  tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                                      <  2:1272802/70‑1 (MQ=255)
                                                                                                  tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACAt                                                           <  2:283867/97‑1 (MQ=255)
                                                                                                  tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACAt                                                           >  1:283867/1‑97 (MQ=255)
                                                                                                  tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAg                 >  2:861938/1‑139 (MQ=255)
                                                                                                  tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAg                 >  1:1034345/1‑139 (MQ=255)
                                                                                                       tATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAg            >  2:646315/1‑139 (MQ=255)
                                                                                                          tACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAc         <  1:646315/139‑1 (MQ=255)
                                                                                                              gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGt                                                                                                     <  1:760217/43‑1 (MQ=255)
                                                                                                              gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGt                                                                                                     >  2:760217/1‑43 (MQ=255)
                                                                                                              gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                    >  2:1518983/1‑60 (MQ=255)
                                                                                                              gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                    <  1:1518983/60‑1 (MQ=255)
                                                                                                                tGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGc   <  1:566030/139‑1 (MQ=255)
                                                                                                                 ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                         <  1:68728/52‑1 (MQ=255)
                                                                                                                 ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                         >  2:68728/1‑52 (MQ=255)
                                                                                                                 ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCt  >  1:1603156/1‑139 (MQ=255)
                                                                                                                     |                                                                                                                                      
TATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCT  >  NC_000913/967001‑967252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A