Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	967,118	A→G	Q166Q (CAA→CAG)	msbA →	lipid ABC transporter permease/ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	967,118	0	A	G	100.0%	203.0 / NA	56	Q166Q (CAA→CAG)	msbA	lipid ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/27); total (29/27)

TACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATG  >  NC_000913/966986‑967250

tACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCg                                                                                                                                <  2:927158/139‑1 (MQ=255)

tACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCg                                                                                                                                >  2:178455/1‑139 (MQ=255)

tACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCg                                                                                                                                >  1:927158/1‑139 (MQ=255)

                         gATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGa                                                                                                       >  1:506611/1‑139 (MQ=255)

                                     gTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTg                                                                                           >  1:973143/1‑139 (MQ=255)

                                        gCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGa                                                                                        <  1:178455/139‑1 (MQ=255)

                                          ttcttcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAtt                                                                                      <  2:963369/139‑1 (MQ=255)

                                                     ccGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATc                                                                           >  1:36176/1‑139 (MQ=255)

                                                                 ttACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATGGTTCTGGCACAGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCg                                                               <  2:634743/139‑1 (MQ=255)

                                                                         gtgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCa                                                       >  2:371308/1‑139 (MQ=255)

                                                                          tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                       >  2:763847/1‑106 (MQ=255)

                                                                          tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                       <  1:763847/106‑1 (MQ=255)

                                                                          tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACAt                                                         >  2:583914/1‑136 (MQ=255)

                                                                          tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACAt                                                         <  1:583914/136‑1 (MQ=255)

                                                                          tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAg                                                      <  2:506611/139‑1 (MQ=255)

                                                                          tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAg                                                      >  2:306038/1‑139 (MQ=255)

                                                                              tGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTaaa                                                  >  1:197472/1‑139 (MQ=255)

                                                                               gAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTaaaa                                                 <  2:412976/139‑1 (MQ=255)

                                                                               gAAGGTGCGTCGATCATCGGCCTGTTCATCATGATCTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTaaaa                                                 <  1:649442/139‑1 (MQ=255)

                                                                                    tGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATg                                            >  1:800392/1‑139 (MQ=255)

                                                                                    tGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATg                                            <  1:835385/139‑1 (MQ=255)

                                                                                      cGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCa                                          <  1:534547/139‑1 (MQ=255)

                                                                                      cGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCa                                          >  2:534547/1‑139 (MQ=255)

                                                                                      cGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCa                                          >  1:264305/1‑139 (MQ=255)

                                                                                        tCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGa                                        <  2:800392/139‑1 (MQ=255)

                                                                                         cGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                       <  2:264305/139‑1 (MQ=255)

                                                                                           atcatcGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAaca                                     >  2:1043574/1‑139 (MQ=255)

                                                                                                  gCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCaa                                                            >  2:486896/1‑109 (MQ=255)

                                                                                                  gCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCaa                                                            <  1:486896/109‑1 (MQ=255)

                                                                                                    cTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                            <  1:371308/139‑1 (MQ=255)

                                                                                                      gTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGAt                                                                                                                              <  2:824721/39‑1 (MQ=255)

                                                                                                      gTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGAt                                                                                                                              >  1:824721/1‑39 (MQ=255)

                                                                                                       ttCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAgg                         >  1:389710/1‑139 (MQ=255)

                                                                                                        tcatcaTGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAg                                                      <  2:661297/109‑1 (MQ=255)

                                                                                                        tcatcaTGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAg                                                      >  1:661297/1‑109 (MQ=255)

                                                                                                        tcatcaTGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGt                        <  1:306038/139‑1 (MQ=255)

                                                                                                            catGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGacc                    >  1:466801/1‑139 (MQ=255)

                                                                                                              tgatgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGaccac                  <  2:389710/139‑1 (MQ=255)

                                                                                                               gatgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGaccacc                 >  2:94887/1‑139 (MQ=255)

                                                                                                                         tACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAc       <  1:1043574/139‑1 (MQ=255)

                                                                                                                         tACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAc       <  1:94887/139‑1 (MQ=255)

                                                                                                                         tACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAc       <  2:197472/139‑1 (MQ=255)

                                                                                                                           cAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACaa     >  1:88939/1‑139 (MQ=255)

                                                                                                                             gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAAt   >  1:422685/1‑139 (MQ=255)

                                                                                                                             gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAAt   >  1:147563/1‑139 (MQ=255)

                                                                                                                              ttGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                  <  1:81461/59‑1 (MQ=255)

                                                                                                                              ttGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                  >  2:81461/1‑59 (MQ=255)

                                                                                                                              ttGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATg  >  1:773979/1‑139 (MQ=255)

                                                                                                                                ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                       >  1:708945/1‑52 (MQ=255)

                                                                                                                                ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                       >  2:41486/1‑100 (MQ=255)

                                                                                                                                ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                       <  1:41486/100‑1 (MQ=255)

                                                                                                                                ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                       <  1:1049703/100‑1 (MQ=255)

                                                                                                                                ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                       >  2:1049703/1‑100 (MQ=255)

                                                                                                                                ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAgcg             >  1:665073/1‑126 (MQ=255)

                                                                                                                                ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAgcg             <  2:665073/126‑1 (MQ=255)

                                                                                                                                ggCAGCTGTCGAACATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAt                                                                                       <  2:708945/52‑1 (MQ=39)

TACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATG  >  NC_000913/966986‑967250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A