Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I2 R1 766 64.2 3232166 90.9% 2938038 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 988,952 C→T G12S (GGC→AGC)  asnS ← asparaginyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913988,9520CT98.4% 192.6 / NA 62G12S (GGC→AGC) asnSasparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (30/31);  minor base G (1/0);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGACAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTGCAATCAGACAAG  >  NC_000913/988824‑989079
                                                                                                                                |                                                                                                                               
gatgaCAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACg                                                                                                                       <  1:232089/139‑1 (MQ=255)
   gaCAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCg                                                                                                                    >  2:888965/1‑139 (MQ=255)
                ggATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACaa                                                                                                       >  2:614356/1‑139 (MQ=255)
                 gATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAAc                                                                                                      >  2:311920/1‑139 (MQ=255)
                 gATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAAc                                                                                                      <  1:311920/139‑1 (MQ=255)
                   tCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGc                                                                                                    <  1:888965/139‑1 (MQ=255)
                    cAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTa                                                                                                                >  2:1182681/1‑126 (MQ=255)
                    cAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTa                                                                                                                <  1:1182681/126‑1 (MQ=255)
                      aaGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCa                                                                                                 >  1:1313908/1‑139 (MQ=255)
                          aGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCataat                                                                                             <  2:1313908/139‑1 (MQ=255)
                            gAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTc                                                                                                  <  2:38356/132‑1 (MQ=255)
                            gAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTc                                                                                                  >  1:38356/1‑132 (MQ=255)
                            gAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAatat                                                                                           >  2:657464/1‑139 (MQ=255)
                             aaCCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATAtt                                                                                          >  2:898907/1‑139 (MQ=255)
                              aCCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGTTACAGGCACAACGCTCATAATATtc                                                                                         <  2:791052/139‑1 (MQ=255)
                                cGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctc                                                                                       <  1:898907/139‑1 (MQ=255)
                                cGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctc                                                                                       <  1:614356/139‑1 (MQ=255)
                                 gTCATAAACGGCGAGGAAGGAGATGCCAGATTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACCCTCATAATATtctct                                                                                      >  2:1077796/1‑139 (MQ=255)
                                  tCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctctc                                                                                     >  1:298783/1‑139 (MQ=255)
                                    aTAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc                                                                                         >  2:1270096/1‑133 (MQ=255)
                                    aTAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc                                                                                         <  1:1270096/133‑1 (MQ=255)
                                        aCGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct                                                                                      <  2:1456044/132‑1 (MQ=255)
                                        aCGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct                                                                                      >  1:1456044/1‑132 (MQ=255)
                                            cGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGt                                                                                                                      >  1:42238/1‑96 (MQ=255)
                                            cGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGt                                                                                                                      <  2:42238/96‑1 (MQ=255)
                                            cGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGt                                                                           >  1:73807/1‑139 (MQ=255)
                                            cGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGGGACTTCGCTGTCAACGGCTACACGGCGCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCCGTTAATAGt                                                                           >  1:744736/1‑139 (MQ=255)
                                                 aaggaGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGaa                                                                      <  2:15048/139‑1 (MQ=255)
                                                  aggaGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGaaa                                                                     <  2:73807/139‑1 (MQ=255)
                                                       aTGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct                                                                                      >  2:1544506/1‑117 (MQ=255)
                                                       aTGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct                                                                                      <  1:1544506/117‑1 (MQ=255)
                                                           cAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAaca                                                            <  2:298783/139‑1 (MQ=255)
                                                              cTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACtt                                                         >  2:391190/1‑139 (MQ=255)
                                                                 ttGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAATGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAATAAATAAACACTTGTc                                                      <  1:1077796/139‑1 (MQ=255)
                                                                 ttGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTc                                                      <  1:113231/139‑1 (MQ=255)
                                                                           gcggGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTaaa                                            >  2:474824/1‑139 (MQ=255)
                                                                                tacgtacCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATgggg                                       <  1:643677/139‑1 (MQ=255)
                                                                                 acgtacCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAGTAGTCGCTCTGTTAATAGTCGGAAAAAATAAACGCTTGGCCACCCGTAAATggggg                                      <  2:744736/139‑1 (MQ=255)
                                                                                    tacCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGAt                                   >  2:1363719/1‑139 (MQ=255)
                                                                                         aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACaa                                                                                                       <  1:361649/66‑1 (MQ=255)
                                                                                         aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACaa                                                                                                       >  2:361649/1‑66 (MQ=255)
                                                                                         aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATggggg                                      <  1:371253/131‑1 (MQ=255)
                                                                                         aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATggggg                                      >  2:371253/1‑131 (MQ=255)
                                                                                         aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTa                              >  1:395708/1‑139 (MQ=255)
                                                                                          tCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc                                                                                         >  2:124112/1‑79 (MQ=255)
                                                                                          tCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc                                                                                         <  1:124112/79‑1 (MQ=255)
                                                                                          tCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTAt                             >  2:565655/1‑139 (MQ=255)
                                                                                           ccGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGc                                                                                                    <  2:690165/67‑1 (MQ=255)
                                                                                           ccGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGc                                                                                                    >  1:690165/1‑67 (MQ=255)
                                                                                              cgcACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATaaa                                                              >  2:901172/1‑102 (MQ=255)
                                                                                              cgcACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATaaa                                                              <  1:901172/102‑1 (MQ=255)
                                                                                                   ggTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATaaa                                                              >  1:1391039/1‑97 (MQ=255)
                                                                                                   ggTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATaaa                                                              <  2:1391039/97‑1 (MQ=255)
                                                                                                     tGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCa                  >  2:1299943/1‑139 (MQ=255)
                                                                                                           cGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc                                                                                         >  1:1098950/1‑62 (MQ=255)
                                                                                                           cGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc                                                                                         <  2:1098950/62‑1 (MQ=255)
                                                                                                             cTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTaaa                                            >  2:338091/1‑105 (MQ=255)
                                                                                                             cTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTaaa                                            <  1:338091/105‑1 (MQ=255)
                                                                                                                  aaCGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct                                                                                      >  1:905125/1‑58 (MQ=255)
                                                                                                                  aaCGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct                                                                                      <  2:905125/58‑1 (MQ=255)
                                                                                                                     ggCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTGCAATCAGACAAg  <  1:391190/139‑1 (MQ=255)
                                                                                                                     ggCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTGCAATCAGACAAg  <  1:1363719/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                               
GATGACAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTGCAATCAGACAAG  >  NC_000913/988824‑989079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A