Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
766 |
64.2 |
3232166 |
90.9% |
2938038 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,761,518 |
G→A |
R92C (CGT→TGT) |
sufD ← |
component of SufBCD Fe‑S cluster assembly scaffold |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,761,518 | 0 | G | A | 98.0%
| 156.8
/ ‑6.9
| 50 | R92C (CGT→TGT) | sufD | component of SufBCD Fe‑S cluster assembly scaffold |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/27); minor base C (1/0); total (23/27) |
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTA > NC_000913/1761384‑1761639
|
gCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCa < 1:977603/139‑1 (MQ=255)
ggCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAacac > 2:175675/1‑139 (MQ=255)
tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGCAACACCCATCGACAAACACCAGcc > 2:902138/1‑139 (MQ=255)
tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCAc < 1:733098/139‑1 (MQ=255)
cAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCt < 2:926267/139‑1 (MQ=255)
ttCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAAc > 2:24664/1‑139 (MQ=255)
gAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGc > 2:607930/1‑139 (MQ=255)
cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg > 2:165191/1‑139 (MQ=255)
cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg < 1:175675/139‑1 (MQ=255)
ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgc > 1:1296002/1‑139 (MQ=255)
ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCACTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct < 2:1587340/139‑1 (MQ=255)
aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg > 1:5436/1‑123 (MQ=255)
aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg < 2:5436/123‑1 (MQ=255)
aaTGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCAc < 2:877154/81‑1 (MQ=255)
aaTGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCAc > 1:877154/1‑81 (MQ=255)
tGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCt > 2:192464/1‑139 (MQ=255)
cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct < 2:271814/110‑1 (MQ=255)
cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct > 1:271814/1‑110 (MQ=255)
cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACa > 2:1144141/1‑139 (MQ=255)
cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg < 2:411102/106‑1 (MQ=255)
cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg > 1:411102/1‑106 (MQ=255)
cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa < 1:1144141/139‑1 (MQ=255)
cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa < 2:257115/139‑1 (MQ=255)
ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAAc < 1:902138/139‑1 (MQ=255)
ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAAc < 2:1312263/139‑1 (MQ=255)
cGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc > 1:519617/1‑116 (MQ=255)
cGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc < 2:519617/116‑1 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCt < 2:970612/69‑1 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCt > 1:970612/1‑69 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCaa < 2:508570/118‑1 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCaa > 1:508570/1‑118 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt > 1:770346/1‑130 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt < 2:770346/130‑1 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCt < 2:510410/134‑1 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCt > 1:510410/1‑134 (MQ=255)
cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGa > 2:1522054/1‑139 (MQ=255)
tGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTAACAAACTGGCTATTGAt < 1:165191/139‑1 (MQ=255)
ttCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTa > 1:465544/1‑65 (MQ=255)
ttCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTa < 2:465544/65‑1 (MQ=255)
gCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCa < 2:1354466/139‑1 (MQ=255)
cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACg > 2:510938/1‑50 (MQ=255)
cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACg < 1:510938/50‑1 (MQ=255)
cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt > 1:1436978/1‑117 (MQ=255)
cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt < 2:1436978/117‑1 (MQ=255)
cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAg < 1:607930/139‑1 (MQ=255)
cAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGta < 1:1522054/139‑1 (MQ=255)
tGC‑GGGCACGTAACCCCCATCGACAAACACCAGCCGCAc > 2:803519/1‑39 (MQ=255)
tGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCAc < 1:803519/39‑1 (MQ=255)
tAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCAt > 2:639516/1‑55 (MQ=255)
tAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCAt < 1:639516/55‑1 (MQ=255)
|
GCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTA > NC_000913/1761384‑1761639
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
