Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,761,518	G→A	R92C (CGT→TGT)	sufD ←	component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,761,518	0	G	A	100.0%	106.6 / NA	34	R92C (CGT→TGT)	sufD	component of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/17); total (17/17)

GGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTT  >  NC_000913/1761395‑1761652

ggCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAacac                                                                                                                         >  1:1228411/1‑139 (MQ=255)

     tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCAt                                                                                                                                   <  2:981267/124‑1 (MQ=255)

     tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCAt                                                                                                                                   >  1:981267/1‑124 (MQ=255)

     tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGcc                                                                                                                    >  1:1135009/1‑139 (MQ=255)

                cAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                         <  1:602720/139‑1 (MQ=255)

                     acacTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCCCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGt                                                                                                    >  1:390154/1‑139 (MQ=255)

                                       cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                  <  1:235877/139‑1 (MQ=255)

                                                 aCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTggg                                                                        >  2:335830/1‑139 (MQ=255)

                                                      gACGGTCGTCGTTAATGCTCACTTGATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGatat                                                                   <  1:752659/139‑1 (MQ=255)

                                                            cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                             >  1:1180780/1‑123 (MQ=255)

                                                            cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                             <  2:1180780/123‑1 (MQ=255)

                                                             gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCAc                                                                                                                <  1:1029906/87‑1 (MQ=255)

                                                             gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCAc                                                                                                                >  2:1029906/1‑87 (MQ=255)

                                                             gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc                                                            <  2:1135009/139‑1 (MQ=255)

                                                                         cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGAc                                                 >  1:38511/1‑138 (MQ=255)

                                                                         cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGAc                                                 <  2:38511/138‑1 (MQ=255)

                                                                           cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              <  1:335830/139‑1 (MQ=255)

                                                                             tCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                            <  2:452021/139‑1 (MQ=255)

                                                                                      cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              >  1:443631/1‑128 (MQ=255)

                                                                                      cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              <  2:443631/128‑1 (MQ=255)

                                                                                      cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGa                                   >  2:143922/1‑139 (MQ=255)

                                                                                       tGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                  <  2:1228411/139‑1 (MQ=255)

                                                                                                   cATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                   <  1:1191416/62‑1 (MQ=255)

                                                                                                   cATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                   >  2:1191416/1‑62 (MQ=255)

                                                                                                   cATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCa                                                                                       >  1:593920/1‑74 (MQ=255)

                                                                                                   cATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCa                                                                                       <  2:593920/74‑1 (MQ=255)

                                                                                                   cATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCa                       >  1:765324/1‑138 (MQ=255)

                                                                                                   cATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCa                       <  2:765324/138‑1 (MQ=255)

                                                                                                        cTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcggcg                 >  1:1080068/1‑139 (MQ=255)

                                                                                                                  gCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTa                                                                                                        >  2:1262349/1‑42 (MQ=255)

                                                                                                                  gCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTa                                                                                                        <  1:1262349/42‑1 (MQ=255)

                                                                                                                       tAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGtttt  >  1:1085159/1‑139 (MQ=255)

                                                                                                                       tAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGATAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              >  1:792912/1‑95 (MQ=255)

                                                                                                                       tAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGATAAGGCATCACGCTGCTGTGGGGATATATCTCCCGCAATGCTGACaaa                                              <  2:792912/95‑1 (MQ=255)

GGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTT  >  NC_000913/1761395‑1761652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A