Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,146	C→T	A158V (GCG→GTG)	csiR →	transcriptional repressor of csiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,146	0	C	T	100.0%	189.8 / NA	59	A158V (GCG→GTG)	csiR	transcriptional repressor of csiD
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (33/26); total (33/26)

GAGGCCTGTGACGCCAGCGAGAAAATGCTTGAT‑‑GAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGA  >  NC_000913/2796016‑2796272

gAGGCCTGTGACGCCAGCGAGAAAATGCTTGAT‑‑GAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGtt                                                                                                                        <  2:1197685/139‑1 (MQ=255)

 aGGCCTGTGACGCCAGCGAGAAAATGCTTGAT‑‑GAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTa                                                                                                                       >  1:1556310/1‑139 (MQ=255)

      tgtgACGCCAGCGAGAAAATGCTTGAT‑‑GAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGAt                                                                                                                  >  1:572340/1‑139 (MQ=255)

       gtgACGCCAGCGAGAAAATGCTTGATGAGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGa                                                                                                                   >  1:493799/1‑139 (MQ=255)

        tgACGCCAGCGAGAAAATGCTTGAT‑‑GAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCg                                                                                                                    >  2:1293154/1‑135 (MQ=255)

        tgACGCCAGCGAGAAAATGCTTGAT‑‑GAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCg                                                                                                                    <  1:1293154/135‑1 (MQ=255)

             ccAGCGAGAAAATGCTTGAT‑‑GAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTg                                                                                                           >  2:919892/1‑139 (MQ=255)

                  gagaAAATGCTTGATGAGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCt                                                                                                        <  2:493799/139‑1 (MQ=255)

                                    aGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTACTTTc                                                                                      <  2:1556310/139‑1 (MQ=255)

                                           tCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGaa                                                                               >  1:1400095/1‑139 (MQ=255)

                                              gCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATg                                                                            >  1:1039816/1‑139 (MQ=255)

                                                 tcatcaGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                         <  2:1039816/139‑1 (MQ=255)

                                                 tcatcaGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                         <  1:1079612/139‑1 (MQ=255)

                                                  catcaGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTgg                                                                        >  1:1445881/1‑139 (MQ=255)

                                                   atcaGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTc                                                                                      >  1:1006694/1‑124 (MQ=255)

                                                   atcaGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTCCGGCGAACGGTGCTTTc                                                                                      <  2:1006694/124‑1 (MQ=255)

                                                    tcaGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTggag                                                                      >  1:264858/1‑139 (MQ=255)

                                                       ggCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGAt                                                                   >  1:1275345/1‑139 (MQ=255)

                                                                    gCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCt                                                                                                        >  1:76510/1‑89 (MQ=255)

                                                                    gCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCt                                                                                                        <  2:76510/89‑1 (MQ=255)

                                                                    gCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGc                                                      >  1:86738/1‑139 (MQ=255)

                                                                     cAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGcgcg                                                                                                                          <  1:316617/70‑1 (MQ=255)

                                                                     cAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGcgcg                                                                                                                          >  2:316617/1‑70 (MQ=255)

                                                                       aTTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGgtggcg                                                                                                                            >  1:557295/1‑66 (MQ=255)

                                                                       aTTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGgtggcg                                                                                                                            <  2:557295/66‑1 (MQ=255)

                                                                                  cTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAAc                                                                                               <  2:446348/84‑1 (MQ=255)

                                                                                  cTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAAc                                                                                               >  1:446348/1‑84 (MQ=255)

                                                                                  cTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACt                                        >  1:425156/1‑139 (MQ=255)

                                                                                   tgtgGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGcgcg                                                                                                                          <  2:1538757/56‑1 (MQ=255)

                                                                                   tgtgGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAac                                                               >  2:274533/1‑115 (MQ=255)

                                                                                   tgtgGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAac                                                               <  1:274533/115‑1 (MQ=255)

                                                                                   tgtgGTTCTCACTATTTGCTGCAAATGAGTGAACGGTTGTTTGATCTGGTGGcgcg                                                                                                                          >  1:1538757/1‑56 (MQ=255)

                                                                                     tgGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCAc                                                    >  1:761635/1‑124 (MQ=255)

                                                                                     tgGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCAc                                                    <  2:761635/124‑1 (MQ=255)

                                                                                           tcACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTACTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTa                               >  1:457915/1‑139 (MQ=255)

                                                                                             aCTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACt                             >  1:1363255/1‑139 (MQ=255)

                                                                                                aTTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGgc                          <  2:1275345/139‑1 (MQ=255)

                                                                                                 tttGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGgcg                         >  1:1264927/1‑139 (MQ=255)

                                                                                                  ttGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGgcgc                        <  2:425156/139‑1 (MQ=255)

                                                                                                  ttGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGgcgc                        >  2:1404127/1‑139 (MQ=255)

                                                                                                  ttGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGgcgc                        <  2:1400095/139‑1 (MQ=255)

                                                                                                     ctgcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                         <  2:1226157/87‑1 (MQ=255)

                                                                                                     ctgcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                         >  1:1226157/1‑87 (MQ=255)

                                                                                                     ctgcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCgag                     >  1:339533/1‑139 (MQ=255)

                                                                                                       gcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTc                                                                                      <  2:909450/72‑1 (MQ=255)

                                                                                                       gcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTc                                                                                      >  1:909450/1‑72 (MQ=255)

                                                                                                       gcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt                   >  1:1340661/1‑139 (MQ=255)

                                                                                                         aaaTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                         >  2:661806/1‑83 (MQ=255)

                                                                                                         aaaTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                         <  1:661806/83‑1 (MQ=255)

                                                                                                          aaTGCATGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGTTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATAcc                <  2:1002319/139‑1 (MQ=255)

                                                                                                           aTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCTATTTATCTGGCTGCGGCTAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTTCTGGcgcg                       <  1:1037161/131‑1 (MQ=255)

                                                                                                           aTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTTCTGGcgcg                       >  2:1037161/1‑131 (MQ=255)

                                                                                                           aTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTAc                              >  2:391031/1‑124 (MQ=255)

                                                                                                           aTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTAc                              <  1:391031/124‑1 (MQ=255)

                                                                                                                       ttgttTGATCTGGTGGCGCGTTATCGATTTATCTgg                                                                                                          <  1:1308225/36‑1 (MQ=255)

                                                                                                                       ttgttTGATCTGGTGGCGCGTTATCGATTTATCTgg                                                                                                          >  2:1308225/1‑36 (MQ=255)

                                                                                                                       ttgttTGATCTGGTGGCGCGTTATCGATTTATCTGGCt                                                                                                        <  1:1506081/38‑1 (MQ=255)

                                                                                                                       ttgttTGATCTGGTGGCGCGTTATCGATTTATCTGGCt                                                                                                        >  2:1506081/1‑38 (MQ=38)

                                                                                                                       ttgttTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATaa                                                                 <  2:317884/77‑1 (MQ=255)

                                                                                                                       ttgttTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATaa                                                                 >  1:317884/1‑77 (MQ=255)

                                                                                                                        tgttTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGa  <  2:1340661/139‑1 (MQ=255)

                                                                                                                                 tggtggCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTa                               <  2:343840/101‑1 (MQ=255)

                                                                                                                                 tggtggCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACcgcgcg           <  1:1383849/121‑1 (MQ=255)

                                                                                                                                 tggtggCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCa      >  1:320670/1‑126 (MQ=255)

                                                                                                                                 tggtggCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGAAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTa                               >  1:343840/1‑101 (MQ=255)

GAGGCCTGTGACGCCAGCGAGAAAATGCTTGAT‑‑GAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGA  >  NC_000913/2796016‑2796272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A