Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,213,680	C→T	148 (TAG→TAA)	yjaB ←	GNAT‑family putative N‑acetyltransferase, acetyl coenzyme A‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,213,680	0	C	T	98.7%	249.0 / NA	78	148 (TAG→TAA)	yjaB	GNAT‑family putative N‑acetyltransferase, acetyl coenzyme A‑binding protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (37/40); total (38/40)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTG  >  NC_000913/4213549‑4213812

ttCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                >  1:267494/1‑139 (MQ=255)

     caTCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGcc                                                                                                                                 <  1:792628/133‑1 (MQ=255)

     caTCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGcc                                                                                                                                 >  2:792628/1‑133 (MQ=255)

           tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                <  2:176925/128‑1 (MQ=255)

           tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                >  1:176925/1‑128 (MQ=255)

           tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                     >  1:1561950/1‑139 (MQ=255)

                  tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCa                                                                                                              >  2:421262/1‑139 (MQ=255)

                    aGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                            <  1:479089/139‑1 (MQ=255)

                     gCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCGCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCa                                                                                                           <  1:213311/139‑1 (MQ=255)

                      cGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCaa                                                                                                          >  1:1264719/1‑139 (MQ=255)

                              aGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTAc                                                                                                                                    >  1:807997/1‑105 (MQ=255)

                              aGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTAc                                                                                                                                    <  2:807997/105‑1 (MQ=255)

                                     gTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑ccc                                                                                          >  2:1594724/1‑139 (MQ=255)

                                      tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑cccc                                                                                         >  2:1009440/1‑139 (MQ=255)

                                      tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑cccc                                                                                         >  1:1275250/1‑139 (MQ=255)

                                        cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                <  2:85688/99‑1 (MQ=255)

                                        cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                >  1:85688/1‑99 (MQ=255)

                                          gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                >  2:1604419/1‑97 (MQ=255)

                                          gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                <  1:1604419/97‑1 (MQ=255)

                                          gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAAt                                                                                     >  1:209539/1‑139 (MQ=255)

                                             aTGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                     <  1:856825/105‑1 (MQ=255)

                                             aTGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                     >  2:856825/1‑105 (MQ=255)

                                              tGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTc                                                                                 <  1:1435965/139‑1 (MQ=255)

                                              tGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTc                                                                                 <  1:421262/139‑1 (MQ=255)

                                               ggCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCCAAATCGTc                                                                                 <  1:372867/139‑1 (MQ=255)

                                                gCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCa                                                                               <  2:1275250/139‑1 (MQ=255)

                                                  aaTGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCAcc                                                                             <  2:114055/139‑1 (MQ=255)

                                                        tAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCagag                                                                       <  2:267494/139‑1 (MQ=255)

                                                               aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCaa                                                                                                          >  2:198267/1‑98 (MQ=255)

                                                               aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCaa                                                                                                          <  1:198267/98‑1 (MQ=255)

                                                               aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCg                                                                >  1:256664/1‑139 (MQ=255)

                                                                aataatCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               <  2:256664/139‑1 (MQ=255)

                                                                         gtCGCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGGGTCCCGTAACCTTaaa                                                      >  2:1097854/1‑139 (MQ=255)

                                                                         gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCCGATTCAGCAACGGATACGGTTT‑CCCCaaa                                                                                      <  2:1076305/107‑1 (MQ=255)

                                                                         gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAGCGGATACGGTTT‑CCCCaaa                                                                                      >  1:1076305/1‑107 (MQ=255)

                                                                         gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaa                                                      >  1:263233/1‑139 (MQ=255)

                                                                            aCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                   <  2:1561950/139‑1 (MQ=255)

                                                                               aTTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCcaca                                                                                                                           >  1:1187257/1‑65 (MQ=255)

                                                                               aTTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCcaca                                                                                                                           <  2:1187257/65‑1 (MQ=255)

                                                                               aTTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCAc                                                <  2:263233/139‑1 (MQ=255)

                                                                                ttGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCAcc                                                                             >  1:638754/1‑109 (MQ=255)

                                                                                ttGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCAcc                                                                             <  2:638754/109‑1 (MQ=255)

                                                                                 tggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAGCCCACct                                              <  1:368989/139‑1 (MQ=255)

                                                                                  ggtggGTACTAAACCTGAGGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCa                                                                                                           >  1:894944/1‑78 (MQ=255)

                                                                                  ggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCa                                                                                                           <  2:894944/78‑1 (MQ=255)

                                                                                   gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               <  2:665880/120‑1 (MQ=255)

                                                                                   gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               >  1:665880/1‑120 (MQ=255)

                                                                                   gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttc                                            >  2:757562/1‑139 (MQ=255)

                                                                                        tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAGATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑cccc                                                                                         >  2:1078649/1‑89 (MQ=255)

                                                                                        tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAATGGATACGGTTT‑cccc                                                                                         <  1:1078649/89‑1 (MQ=255)

                                                                                        tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttc                                            >  2:454883/1‑134 (MQ=255)

                                                                                        tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttc                                            <  1:454883/134‑1 (MQ=255)

                                                                                        tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTtata                                       >  2:1035735/1‑139 (MQ=255)

                                                                                             aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTAc                                                                                                                                    >  1:852318/1‑42 (MQ=255)

                                                                                             aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTAc                                                                                                                                    <  2:852318/42‑1 (MQ=255)

                                                                                             aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGc                                                                      <  2:1268978/103‑1 (MQ=255)

                                                                                             aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGc                                                                      >  1:1268978/1‑103 (MQ=255)

                                                                                             aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCt                                                          >  2:251808/1‑115 (MQ=255)

                                                                                             aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCt                                                          <  1:251808/115‑1 (MQ=255)

                                                                                                  gAAGTTCAGCCCACCGGGATGCGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCCAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAAcc                             >  1:1122599/1‑139 (MQ=255)

                                                                                                  gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                   >  1:301242/1‑117 (MQ=255)

                                                                                                  gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                   <  2:301242/117‑1 (MQ=255)

                                                                                                  gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                 >  2:174401/1‑119 (MQ=255)

                                                                                                  gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                 <  1:174401/119‑1 (MQ=255)

                                                                                                  gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAAcc                             >  1:224172/1‑139 (MQ=255)

                                                                                                        cAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTc                                                                                 >  2:232815/1‑81 (MQ=255)

                                                                                                        cAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTc                                                                                 <  1:232815/81‑1 (MQ=255)

                                                                                                        cAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGc                       <  2:224172/139‑1 (MQ=255)

                                                                                                        cAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGc                       >  2:656336/1‑139 (MQ=255)

                                                                                                         aGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCt                      <  2:443523/139‑1 (MQ=255)

                                                                                                         aGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCt                      >  2:690796/1‑139 (MQ=255)

                                                                                                           cccACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCg                                                                     >  2:1399570/1‑90 (MQ=255)

                                                                                                           cccACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCg                                                                     <  1:1399570/90‑1 (MQ=255)

                                                                                                               ccGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCAtttt                <  1:1594724/139‑1 (MQ=255)

                                                                                                               ccGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCAtttt                <  1:690796/139‑1 (MQ=255)

                                                                                                                cGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg               <  2:209539/139‑1 (MQ=255)

                                                                                                                  ggATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCt             <  1:757562/139‑1 (MQ=255)

                                                                                                                             aaTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgttg  <  1:656336/139‑1 (MQ=255)

TTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTT‑CCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTG  >  NC_000913/4213549‑4213812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A