Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,213,680	C→T	148 (TAG→TAA)	yjaB ←	GNAT‑family putative N‑acetyltransferase, acetyl coenzyme A‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,213,680	0	C	T	100.0%	164.2 / NA	52	148 (TAG→TAA)	yjaB	GNAT‑family putative N‑acetyltransferase, acetyl coenzyme A‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/25); total (27/25)

CCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGG  >  NC_000913/4213545‑4213813

ccAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTAc                                                                                                                                    <  1:1132714/139‑1 (MQ=255)

ccAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTAc                                                                                                                                    >  1:583576/1‑139 (MQ=255)

               tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                     >  1:711376/1‑139 (MQ=255)

                 cgcAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAg                                                                                                                   >  2:1198648/1‑139 (MQ=255)

                    aaTCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACg                                                                                                                                   <  1:1204625/120‑1 (MQ=255)

                    aaTCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACg                                                                                                                                   >  2:1204625/1‑120 (MQ=255)

                      tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCa                                                                                                              >  1:1104103/1‑139 (MQ=255)

                        aGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                            <  2:583576/139‑1 (MQ=255)

                                   gTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                       >  2:187414/1‑117 (MQ=255)

                                   gTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                       <  1:187414/117‑1 (MQ=255)

                                    tGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGt                                                                                                >  2:971137/1‑139 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGc                                                                                                                      <  1:1171762/114‑1 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGc                                                                                                                      >  2:1171762/1‑114 (MQ=255)

                                         gTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTccc                                                                                           >  1:210474/1‑139 (MQ=255)

                                          tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACgg                                                                                                 >  2:179046/1‑132 (MQ=255)

                                          tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACgg                                                                                                 <  1:179046/132‑1 (MQ=255)

                                          tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcccc                                                                                          >  2:63896/1‑139 (MQ=255)

                                                  tGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  2:711376/139‑1 (MQ=255)

                                                  tGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  1:63896/139‑1 (MQ=255)

                                                  tGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  1:971137/139‑1 (MQ=255)

                                                        tGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGGTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTc                                                                            >  1:746430/1‑139 (MQ=255)

                                                               gCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCcac                                                                                                                            <  1:283783/84‑1 (MQ=255)

                                                               gCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCcac                                                                                                                            >  2:283783/1‑84 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCg                                                                 >  2:755002/1‑139 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCg                                                                 >  2:152102/1‑139 (MQ=255)

                                                                    aataatCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                                <  1:1198648/139‑1 (MQ=255)

                                                                    aataatCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                                >  2:569217/1‑139 (MQ=255)

                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCcac                                                                                                                            >  2:638719/1‑70 (MQ=255)

                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCcac                                                                                                                            <  1:638719/70‑1 (MQ=255)

                                                                              tCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACAt                                                                                                                          >  2:373427/1‑71 (MQ=255)

                                                                              tCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACAt                                                                                                                          <  1:373427/71‑1 (MQ=255)

                                                                              tCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaaa                                                      >  2:803536/1‑139 (MQ=255)

                                                                                aCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  >  2:719163/1‑109 (MQ=255)

                                                                                aCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  1:719163/109‑1 (MQ=255)

                                                                                aCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                    <  1:294670/139‑1 (MQ=255)

                                                                                  cATTGGTGGGTACTTAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACCTACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  2:746430/139‑1 (MQ=255)

                                                                                     tggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACct                                               <  2:1104103/139‑1 (MQ=255)

                                                                                       gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttc                                             >  2:481948/1‑139 (MQ=255)

                                                                                          ggTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGc                                                                       >  1:468136/1‑110 (MQ=255)

                                                                                          ggTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGc                                                                       <  2:468136/110‑1 (MQ=255)

                                                                                                 aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                       >  1:456074/1‑55 (MQ=255)

                                                                                                 aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                       <  2:456074/55‑1 (MQ=255)

                                                                                                     tGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAAc                               <  1:569217/139‑1 (MQ=255)

                                                                                                      gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAAcc                              >  2:30562/1‑139 (MQ=255)

                                                                                                       aaGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCg                             >  2:354338/1‑139 (MQ=255)

                                                                                                             aGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACct                                               >  2:473328/1‑115 (MQ=255)

                                                                                                             aGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACct                                               <  1:473328/115‑1 (MQ=255)

                                                                                                               cccACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCa                     >  2:750704/1‑139 (MQ=255)

                                                                                                                    cGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg                <  1:755002/139‑1 (MQ=255)

                                                                                                                           agaAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTa         <  1:152102/139‑1 (MQ=255)

                                                                                                                                 aaTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgttg   <  1:750704/139‑1 (MQ=255)

                                                                                                                                  aTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgttgg  <  1:6493/139‑1 (MQ=255)

CCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGG  >  NC_000913/4213545‑4213813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A