Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	444,641	C→T	intergenic (‑126/‑42)	panE ← / → yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	444,641	0	C	T	100.0%	129.3 / NA	41	intergenic (‑126/‑42)	panE/yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/21); total (20/21)

TAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGTTTTGAC  >  NC_000913/444506‑444775

tAATTTTCATTGTTCCTCCTCACCCACAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTggg                                                                                                                                     >  2:945683/1‑139 (MQ=255)

              cctcctCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCa                                                                                                                       >  1:1114063/1‑139 (MQ=255)

                      cccGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCAt                                                                                                                      >  2:342965/1‑132 (MQ=255)

                      cccGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCAt                                                                                                                      <  1:342965/132‑1 (MQ=255)

                                            aaTAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                                         <  1:945683/139‑1 (MQ=255)

                                                aGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCtt                                                                                     <  1:173861/139‑1 (MQ=255)

                                                   cTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCg                                                                                  >  2:752779/1‑139 (MQ=255)

                                                        ccTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATAtt                                                                             <  1:33347/139‑1 (MQ=255)

                                                                 tgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                                         <  1:125454/118‑1 (MQ=255)

                                                                 tgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                                         >  2:125454/1‑118 (MQ=255)

                                                                 tgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGaa                                                                    >  2:904372/1‑139 (MQ=255)

                                                                 tgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGaa                                                                    >  1:969792/1‑139 (MQ=255)

                                                                  gCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAg                                                                   <  1:752779/139‑1 (MQ=255)

                                                                  gCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAg                                                                   >  2:1025752/1‑139 (MQ=255)

                                                                           tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAg                                                                                               <  2:658466/102‑1 (MQ=255)

                                                                           tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAg                                                                                               >  1:658466/1‑102 (MQ=255)

                                                                           tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTc                                                          >  1:1155159/1‑139 (MQ=255)

                                                                           tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTc                                                          <  2:1114063/139‑1 (MQ=255)

                                                                               aCTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGa                                                      <  2:969792/139‑1 (MQ=255)

                                                                                   tGCACAATTAAGGTATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCa                                                  <  1:904372/139‑1 (MQ=255)

                                                                                             aGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGa                                                      >  1:22571/1‑125 (MQ=255)

                                                                                             aGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGa                                                      <  2:22571/125‑1 (MQ=255)

                                                                                                    cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCa                                                  >  2:997351/1‑122 (MQ=255)

                                                                                                    cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCa                                                  <  1:997351/122‑1 (MQ=255)

                                                                                                          ggTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAg                           <  1:766985/139‑1 (MQ=255)

                                                                                                          ggTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAg                           >  2:49521/1‑139 (MQ=255)

                                                                                                                ggACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGa                     >  2:205098/1‑139 (MQ=255)

                                                                                                                 gACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGaa                    >  1:125199/1‑139 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAgg                                                                                                          >  2:632282/1‑48 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAgg                                                                                                          <  1:632282/48‑1 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAAcgcg                                         >  1:560282/1‑113 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAAcgcg                                         <  2:560282/113‑1 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGcc                         <  2:1214266/129‑1 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGcc                         >  1:1214266/1‑129 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGa               >  2:822983/1‑139 (MQ=255)

                                                                                                                             tttAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAAcgc                                          >  1:909664/1‑105 (MQ=255)

                                                                                                                             tttAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAAcgc                                          <  2:909664/105‑1 (MQ=255)

                                                                                                                             tttAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGt        <  1:205098/139‑1 (MQ=255)

                                                                                                                                aaCTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGtttt     <  2:10378/139‑1 (MQ=255)

                                                                                                                                aaCTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGtttt     <  1:1025752/139‑1 (MQ=255)

                                                                                                                                   tttGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGTTTTGAc  <  2:125199/139‑1 (MQ=255)

TAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGTTTTGAC  >  NC_000913/444506‑444775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A