Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	444,641	C→T	intergenic (‑126/‑42)	panE ← / → yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	444,641	0	C	T	100.0%	160.9 / NA	51	intergenic (‑126/‑42)	panE/yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/25); total (26/25)

TAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGTTTTGA  >  NC_000913/444506‑444774

tAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTggg                                                                                                                                    >  1:107448/1‑139 (MQ=255)

tAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTggg                                                                                                                                    >  1:336480/1‑139 (MQ=255)

               ctcctcACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCAt                                                                                                                     <  1:209872/139‑1 (MQ=255)

                 cctcACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCa                                                                                                                   >  2:264031/1‑139 (MQ=255)

                           aaCCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAgg                                                                                                         <  2:336480/139‑1 (MQ=255)

                                 tCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAgg                                                                                                   >  2:103099/1‑139 (MQ=255)

                                   cGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGaa                                                                                                 <  2:436883/139‑1 (MQ=255)

                                        gtTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAgg                                                                                                         >  1:14643/1‑126 (MQ=255)

                                        gtTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAgg                                                                                                         <  2:14643/126‑1 (MQ=255)

                                         ttCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATg                                                                                           >  2:145060/1‑139 (MQ=255)

                                          tCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGc                                                                                          <  1:103099/139‑1 (MQ=255)

                                            aaTAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                                        <  1:264031/139‑1 (MQ=255)

                                                aGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCtt                                                                                    <  1:145060/139‑1 (MQ=255)

                                                 ggCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCtt                                                                                                                          <  2:868646/100‑1 (MQ=255)

                                                 ggCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCtt                                                                                                                          >  1:868646/1‑100 (MQ=255)

                                                   cTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                                         <  2:95863/131‑1 (MQ=255)

                                                   cTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                                         >  1:95863/1‑131 (MQ=255)

                                                     aTCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGaa                                                                                                           <  1:1010124/111‑1 (MQ=255)

                                                     aTCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGaa                                                                                                           >  2:1010124/1‑111 (MQ=255)

                                                     aTCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGat                                                                               <  2:925336/139‑1 (MQ=255)

                                                      tCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGata                                                                              >  1:371995/1‑139 (MQ=255)

                                                         cTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTgg                                                                                                                                     >  1:483597/1‑81 (MQ=255)

                                                            aaTTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtct                                                                        <  2:371995/139‑1 (MQ=255)

                                                                  gCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAg                                                                  >  1:269284/1‑139 (MQ=255)

                                                                   cATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                                 <  2:107448/139‑1 (MQ=255)

                                                                           tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTc                                                         >  1:1131923/1‑139 (MQ=255)

                                                                           tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTc                                                         >  2:421834/1‑139 (MQ=255)

                                                                           tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTc                                                         <  1:421834/139‑1 (MQ=255)

                                                                             cGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCttt                                                                                   >  2:596953/1‑111 (MQ=255)

                                                                             cGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCttt                                                                                   <  1:596953/111‑1 (MQ=255)

                                                                                   tGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCa                                                 <  2:475377/139‑1 (MQ=255)

                                                                                          ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCtt                                                                                                                          <  2:945888/59‑1 (MQ=255)

                                                                                          ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCtt                                                                                                                          >  1:945888/1‑59 (MQ=255)

                                                                                          ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGtt                                                                <  2:95537/117‑1 (MQ=255)

                                                                                          ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGtt                                                                >  1:95537/1‑117 (MQ=255)

                                                                                             aGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATAtt                                                                            >  1:465269/1‑102 (MQ=255)

                                                                                             aGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATAtt                                                                            <  2:465269/102‑1 (MQ=255)

                                                                                                          ggTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATc                                                            >  2:608018/1‑105 (MQ=255)

                                                                                                          ggTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATc                                                            <  1:608018/105‑1 (MQ=255)

                                                                                                                ggACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtctc                                                                       <  1:52444/88‑1 (MQ=255)

                                                                                                                ggACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtctc                                                                       >  2:52444/1‑88 (MQ=255)

                                                                                                                 gACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                                         >  2:88858/1‑69 (MQ=255)

                                                                                                                 gACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                                         <  1:88858/69‑1 (MQ=255)

                                                                                                                     gTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGa                                                              <  2:794691/92‑1 (MQ=255)

                                                                                                                     gTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGa                                                              >  1:794691/1‑92 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGa              >  2:1071453/1‑139 (MQ=255)

                                                                                                                      tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGa              >  2:1052186/1‑139 (MQ=255)

                                                                                                                         gTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTc           <  1:1052186/139‑1 (MQ=255)

                                                                                                                             tttAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                             <  2:67783/69‑1 (MQ=255)

                                                                                                                             tttAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                             >  1:67783/1‑69 (MQ=255)

                                                                                                                                  cTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGTTTTGa  >  2:883061/1‑139 (MQ=255)

TAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGTTTTGA  >  NC_000913/444506‑444774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A