Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I1 R1 770 51.4 2439994 93.8% 2288714 110.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 610,746 C→T A583A (GCG→GCA)  fepA ← ferrienterobactin outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913610,7460CT100.0% 206.8 / NA 64A583A (GCG→GCA) fepAferrienterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/30);  total (34/30)

GGATGATCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACCGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCG  >  NC_000913/610622‑610877
                                                                                                                            |                                                                                                                                   
ggATGATCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGtt                                                                                                                       <  1:651348/139‑1 (MQ=255)
 gatgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTa                                                                                                                      <  1:218315/139‑1 (MQ=255)
 gatgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTa                                                                                                                      >  2:218315/1‑139 (MQ=255)
  atgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  1:1064413/139‑1 (MQ=255)
  atgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  2:437165/139‑1 (MQ=255)
  atgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  1:681432/139‑1 (MQ=255)
    gatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATcc                                                                                                                   <  2:343506/139‑1 (MQ=255)
     atCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     >  1:973641/1‑136 (MQ=255)
     atCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  2:973641/136‑1 (MQ=255)
      tCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCa                                                                                                                 <  2:185739/139‑1 (MQ=255)
                gTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAgaga                                                                                                       >  1:417813/1‑139 (MQ=255)
                 tCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGAt                                                                                                      <  2:444527/139‑1 (MQ=255)
                                       tctTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGccc                                                                                <  2:1133670/139‑1 (MQ=255)
                                       tctTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGccc                                                                                <  1:495398/139‑1 (MQ=255)
                                           ctgCATATATGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTTCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACa                                                                            <  1:512105/137‑1 (MQ=255)
                                           cAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCa                                                                                                                 >  2:362287/1‑102 (MQ=255)
                                           cAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCa                                                                                                                 <  1:362287/102‑1 (MQ=255)
                                            aGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTAGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAg                                                                           >  2:806610/1‑139 (MQ=255)
                                                 ataAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCTGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCcaca                                                                      <  1:806610/139‑1 (MQ=255)
                                                 ataAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCAc                                                                                                                >  1:1006435/1‑97 (MQ=255)
                                                 ataAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCAc                                                                                                                <  2:1006435/97‑1 (MQ=255)
                                                 ataAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCcaca                                                                      >  2:575469/1‑139 (MQ=255)
                                                     gTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGc                                                                  >  1:779186/1‑139 (MQ=255)
                                                        aTGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     >  1:417175/1‑85 (MQ=255)
                                                        aTGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  2:417175/85‑1 (MQ=255)
                                                        aTGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCTGACTGCGTTTTGCCCTACAGCCACATAGCCCg                                                               >  1:769974/1‑139 (MQ=255)
                                                        aTGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCg                                                               >  2:753438/1‑139 (MQ=255)
                                                             aTTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCa                                                          >  2:945582/1‑139 (MQ=255)
                                                                  tCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGtt                                                                                      <  2:356393/106‑1 (MQ=255)
                                                                  tCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGtt                                                                                      >  1:356393/1‑106 (MQ=255)
                                                                     acaTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAg                                                                           <  2:580370/114‑1 (MQ=255)
                                                                     acaTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAg                                                                           >  1:580370/1‑114 (MQ=255)
                                                                      caTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGc                                                                                                <  1:231435/92‑1 (MQ=255)
                                                                      caTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGc                                                                                                >  2:231435/1‑92 (MQ=255)
                                                                       aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCa                                                                                                                           <  2:223990/64‑1 (MQ=255)
                                                                       aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCa                                                                                                                           >  1:223990/1‑64 (MQ=255)
                                                                       aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACg                                                                                                                         <  1:731597/66‑1 (MQ=255)
                                                                       aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACg                                                                                                                         >  2:731597/1‑66 (MQ=255)
                                                                       aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTa                                                                              >  2:268925/1‑109 (MQ=255)
                                                                       aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTa                                                                              <  1:268925/109‑1 (MQ=255)
                                                                         cACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGa                                              <  2:837179/139‑1 (MQ=255)
                                                                           ccGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATa                                            >  1:1117506/1‑139 (MQ=255)
                                                                           ccGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATa                                            >  2:321313/1‑139 (MQ=255)
                                                                             gTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAAt                                          <  2:1117506/139‑1 (MQ=255)
                                                                                           tACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAg                            >  2:1131004/1‑139 (MQ=255)
                                                                                                  aaCGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTa                                                    <  2:1188186/108‑1 (MQ=255)
                                                                                                  aaCGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTa                                                    >  1:1188186/1‑108 (MQ=255)
                                                                                                   aCGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGAagcc                    >  1:498965/1‑139 (MQ=255)
                                                                                                    cGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGAcgccc                   <  2:171305/139‑1 (MQ=255)
                                                                                                        ccTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCgcca               <  2:399024/139‑1 (MQ=255)
                                                                                                        ccTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCgcca               >  2:507997/1‑139 (MQ=255)
                                                                                                         cTTCCAGACCTTCCACCACTGCTTTCGGCACGTTATCCCCCTGATAGAGATCGGTGACGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGTTGACGCCCgctag              >  1:805129/1‑139 (MQ=255)
                                                                                                         cTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAAt                                          >  2:990984/1‑111 (MQ=255)
                                                                                                         cTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAAt                                          <  1:990984/111‑1 (MQ=255)
                                                                                                         cTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCgccag              >  2:270058/1‑139 (MQ=255)
                                                                                                                 ccTTCAACCACTGCTTTCGGCTCGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATGTTATTCCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCCCCCGt      >  1:733573/1‑139 (MQ=255)
                                                                                                                 ccTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCAGTGCCGACTGCGTTTTGCCCT‑CAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTc     >  1:1044077/1‑139 (MQ=255)
                                                                                                                  cTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGt      <  2:10760/138‑1 (MQ=255)
                                                                                                                  cTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGt      >  1:10760/1‑138 (MQ=255)
                                                                                                                   ttCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcg    >  2:1013689/1‑139 (MQ=255)
                                                                                                                    tCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcgc   >  2:871401/1‑139 (MQ=255)
                                                                                                                     cAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCGGCCACCCGTcgcg  >  2:66643/1‑139 (MQ=255)
                                                                                                                      aaCCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGc                                                                  >  2:1026328/1‑74 (MQ=255)
                                                                                                                      aaCCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGc                                                                  <  1:1026328/74‑1 (MQ=255)
                                                                                                                            |                                                                                                                                   
GGATGATCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACCGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCG  >  NC_000913/610622‑610877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A