Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 771 44.8 2153074 94.1% 2026042 109.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 616,422 A→G S756G (AGC→GGC)  entF → enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913616,4220AG100.0% 62.6 / NA 19S756G (AGC→GGC) entFenterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

AGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT  >  NC_000913/616327‑616556
                                                                                               |                                                                                                                                      
aGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCgg                                                                                             >  2:644871/1‑139 (MQ=255)
                                                cgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATc                                                                      >  2:664203/1‑114 (MQ=255)
                                                cgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATc                                                                      <  1:664203/114‑1 (MQ=255)
                                                               tataTGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAgg                                                                                                    >  2:395119/1‑69 (MQ=255)
                                                               tataTGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAgg                                                                                                    <  1:395119/69‑1 (MQ=255)
                                                                 tatGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGAGTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGc                            >  2:1037842/1‑139 (MQ=255)
                                                                      cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCg                                                >  2:732179/1‑114 (MQ=255)
                                                                      cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCg                                                <  1:732179/114‑1 (MQ=255)
                                                                      cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCg                       >  1:496440/1‑139 (MQ=255)
                                                                      cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCAC‑GGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  2:118991/1‑139 (MQ=255)
                                                                       ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  1:57145/1‑139 (MQ=255)
                                                                       ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  2:1065319/1‑139 (MQ=255)
                                                                         gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTg                    <  2:496440/139‑1 (MQ=255)
                                                                         gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTg                    >  1:309432/1‑139 (MQ=255)
                                                                           cGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTgcc                  >  1:1057777/1‑139 (MQ=255)
                                                                              aaGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTgccgcc               <  1:1065319/139‑1 (MQ=255)
                                                                                  ggcggTAGATGTCGGCTGGTATCCGGCTGTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGgt           <  1:644871/139‑1 (MQ=255)
                                                                                          aTGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCggg   <  1:322031/139‑1 (MQ=255)
                                                                                           tGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGt  <  1:567217/139‑1 (MQ=255)
                                                                                               |                                                                                                                                      
AGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT  >  NC_000913/616327‑616556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A