Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 616,422 A→G S756G (AGC→GGC)  entF → enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913616,4220AG100.0% 233.5 / NA 65S756G (AGC→GGC) entFenterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/31);  total (34/31)

AACAGGTTTTCTGTAGTGGTGAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT  >  NC_000913/616306‑616556
                                                                                                                    |                                                                                                                                      
aaCAGGTTTTCTGTAGTGGTGAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGa                                                                                                                  <  1:169035/139‑1 (MQ=255)
                  gtgAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTgcg                                                                                                >  2:129841/1‑139 (MQ=255)
                  gtgAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTgcg                                                                                                >  1:55522/1‑139 (MQ=255)
                       gCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgca                                                                                           >  2:619560/1‑139 (MQ=255)
                          ttACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        <  1:619560/139‑1 (MQ=255)
                          ttACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        >  1:130922/1‑139 (MQ=255)
                            aCCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGcagcagg                                                                                      >  1:682798/1‑138 (MQ=255)
                                    aTTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGAt                                                                              <  2:130922/139‑1 (MQ=255)
                                          gCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGc                                                                                                                                      >  1:1088095/1‑77 (MQ=255)
                                              cggaTGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTgg                                                                                                          <  1:899798/98‑1 (MQ=255)
                                              cgAATGGCAACAGTTTACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTGGGCGAGGAACTgg                                                                                                          >  2:899798/1‑101 (MQ=255)
                                              cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCg                                                                                                                   <  1:1150994/92‑1 (MQ=255)
                                              cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCg                                                                                                                   >  2:1150994/1‑92 (MQ=255)
                                              cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTgg                                                                                                          <  1:76925/101‑1 (MQ=255)
                                              cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTgg                                                                                                          >  2:76925/1‑101 (MQ=255)
                                              cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCg                                                                    >  1:1005311/1‑139 (MQ=255)
                                                 aTGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 <  1:129841/139‑1 (MQ=255)
                                                             aaCTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 <  2:163810/127‑1 (MQ=255)
                                                             aaCTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 >  1:163810/1‑127 (MQ=255)
                                                                   cgcgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagc                                                                                         >  2:1252794/1‑97 (MQ=255)
                                                                   cgcgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagc                                                                                         <  1:1252794/97‑1 (MQ=255)
                                                                   cgcgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGt                                               >  2:426872/1‑139 (MQ=255)
                                                                     cgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTg                                                                                                                                    >  2:163116/1‑52 (MQ=255)
                                                                     cgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTg                                                                                                                                    <  1:163116/52‑1 (MQ=255)
                                                                            gCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGAt                                      <  2:55522/139‑1 (MQ=255)
                                                                                     atatGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGatgatg                             >  1:441014/1‑139 (MQ=255)
                                                                                       atGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCAGAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCa                           >  2:881280/1‑139 (MQ=255)
                                                                                       atGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCa                           >  2:684639/1‑139 (MQ=255)
                                                                                       atGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCa                           >  2:22982/1‑139 (MQ=255)
                                                                                         ggCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATc                         <  2:1005311/139‑1 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTgcg                                                                                                >  1:316298/1‑66 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTgcg                                                                                                <  2:316298/66‑1 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        <  1:868751/74‑1 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        >  2:868751/1‑74 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcag                                                                                          >  2:732711/1‑72 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcag                                                                                          <  1:732711/72‑1 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTAt                                                                       <  2:1182483/91‑1 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTAt                                                                       >  1:1182483/1‑91 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 >  2:736080/1‑97 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 <  1:736080/97‑1 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACggg                                                      >  2:1235063/1‑108 (MQ=255)
                                                                                           cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACggg                                                      <  1:1235063/108‑1 (MQ=255)
                                                                                            ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAAc                                                                                                             >  2:934455/1‑52 (MQ=255)
                                                                                            ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAAc                                                                                                             <  1:934455/52‑1 (MQ=255)
                                                                                            ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTgg                                                                           >  2:407191/1‑86 (MQ=255)
                                                                                            ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTgg                                                                           <  1:407191/86‑1 (MQ=255)
                                                                                            ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACgggg                                                     >  2:786397/1‑107 (MQ=255)
                                                                                            ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGt                                                     <  1:786397/108‑1 (MQ=255)
                                                                                            ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  2:658291/1‑139 (MQ=255)
                                                                                            ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  2:673556/1‑139 (MQ=255)
                                                                                              gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        >  2:741813/1‑71 (MQ=255)
                                                                                              gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        <  1:741813/71‑1 (MQ=255)
                                                                                              gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATg                                                               <  1:1161087/96‑1 (MQ=255)
                                                                                              gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATg                                                               >  2:1161087/1‑96 (MQ=255)
                                                                                              gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGa                                       <  1:751228/120‑1 (MQ=255)
                                                                                              gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGa                                       >  2:751228/1‑120 (MQ=255)
                                                                                                cGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTgcc                  >  2:1082311/1‑139 (MQ=255)
                                                                                                              gATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCgg    <  1:22982/139‑1 (MQ=255)
                                                                                                               aTGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCggg   <  1:881280/139‑1 (MQ=255)
                                                                                                               aTGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCggg   <  2:441014/139‑1 (MQ=255)
                                                                                                               aTGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCggg   <  1:1236358/139‑1 (MQ=255)
                                                                                                                tGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGt  <  1:684639/139‑1 (MQ=255)
                                                                                                                tGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGt  <  1:426872/139‑1 (MQ=255)
                                                                                                                 gTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAgg                                                                                                    >  1:908383/1‑40 (MQ=255)
                                                                                                                 gTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAgg                                                                                                    <  2:908383/40‑1 (MQ=255)
                                                                                                                    |                                                                                                                                      
AACAGGTTTTCTGTAGTGGTGAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT  >  NC_000913/616306‑616556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A