Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,330,454	G→A	A72V (GCC→GTC)	yciN ←	DUF2498 protein YciN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,330,454	0	G	A	100.0%	111.8 / NA	35	A72V (GCC→GTC)	yciN	DUF2498 protein YciN
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/20); total (15/20)

GCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCG  >  NC_000913/1330328‑1330585

gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                         <  1:1078163/139‑1 (MQ=255)

gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                         <  1:232518/139‑1 (MQ=255)

 ccGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGaa                                                                                                                        <  2:729805/139‑1 (MQ=255)

  cgatcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAAc                                                                                                                       <  2:934448/139‑1 (MQ=255)

            ttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTATGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAaca                                                                                                             <  1:745223/139‑1 (MQ=255)

                       gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCtt                                                                                                  >  1:954801/1‑139 (MQ=255)

                                 ggTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTg                                                                                        >  2:321429/1‑139 (MQ=255)

                                             ttGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCa                                                                                                                                  <  2:334172/85‑1 (MQ=255)

                                             ttGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCa                                                                                                                                  >  1:334172/1‑85 (MQ=255)

                                             ttGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                            >  2:68359/1‑139 (MQ=255)

                                             ttGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                            >  2:100571/1‑139 (MQ=255)

                                              tGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc                                                                           <  2:954801/139‑1 (MQ=255)

                                              tGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc                                                                           <  2:843464/139‑1 (MQ=255)

                                                               gcgAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGACCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc                                                          <  1:321429/139‑1 (MQ=255)

                                                               gcgAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc                                                          <  2:141786/139‑1 (MQ=255)

                                                                        aGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg                                                           >  1:175146/1‑129 (MQ=255)

                                                                        aGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg                                                           <  2:175146/129‑1 (MQ=255)

                                                                            cTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAg                                             <  2:1130973/139‑1 (MQ=255)

                                                                              cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                         <  1:920051/61‑1 (MQ=255)

                                                                              cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                         >  2:920051/1‑61 (MQ=255)

                                                                              cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGc                                                                                             >  1:578370/1‑89 (MQ=255)

                                                                              cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGc                                                                                             <  2:578370/89‑1 (MQ=255)

                                                                              cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGc                                                                                      <  1:540579/96‑1 (MQ=255)

                                                                              cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGc                                                                                      >  2:540579/1‑96 (MQ=255)

                                                                              cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCa                                           >  2:1127070/1‑139 (MQ=255)

                                                                              cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCa                                           >  1:103675/1‑139 (MQ=255)

                                                                                   tttGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCa                                      <  2:84342/139‑1 (MQ=255)

                                                                                     tGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACata                                                                                                                    >  2:867418/1‑59 (MQ=255)

                                                                                     tGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACata                                                                                                                    <  1:867418/59‑1 (MQ=255)

                                                                                                    gatgatATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGcc                     >  1:230009/1‑139 (MQ=255)

                                                                                                         tatTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTgg                >  1:640615/1‑139 (MQ=255)

                                                                                                                   aaaGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagta                                                               <  2:1133964/82‑1 (MQ=255)

                                                                                                                   aaaGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagta                                                               >  1:1133964/1‑82 (MQ=255)

                                                                                                                     aGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTg    <  1:1127070/139‑1 (MQ=255)

                                                                                                                       cacaTGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCg  <  2:230009/139‑1 (MQ=255)

GCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCG  >  NC_000913/1330328‑1330585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A