Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,330,454 G→A A72V (GCC→GTC)  yciN ← DUF2498 protein YciN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,330,4540GA100.0% 232.8 / NA 71A72V (GCC→GTC) yciNDUF2498 protein YciN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/38);  total (33/38)

CGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCG  >  NC_000913/1330327‑1330589
                                                                                                                               |                                                                                                                                       
cgcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTg                                                                                                                              >  1:195111/1‑139 (MQ=255)
 gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             <  1:265494/139‑1 (MQ=255)
 gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             <  1:1380013/139‑1 (MQ=255)
 gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             <  2:1088922/139‑1 (MQ=255)
 gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             <  2:171538/139‑1 (MQ=255)
   cgatcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAAc                                                                                                                           <  2:195111/139‑1 (MQ=255)
   cgatcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAAc                                                                                                                           <  2:266758/139‑1 (MQ=255)
        gatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             >  1:310622/1‑132 (MQ=255)
        gatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             <  2:310622/132‑1 (MQ=255)
                   cGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACcgcg                                                                                                            <  1:713419/138‑1 (MQ=255)
                   cGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACcgcg                                                                                                            >  2:713419/1‑138 (MQ=255)
                        gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAAc                                                                                                                           >  1:709731/1‑118 (MQ=255)
                        gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAAc                                                                                                                           <  2:709731/118‑1 (MQ=255)
                        gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACATCGCGGTGCtt                                                                                                      >  1:1689090/1‑139 (MQ=255)
                           gCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg                                                                                                   >  1:1627890/1‑139 (MQ=255)
                              gCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCt                                                                                                <  2:1627890/139‑1 (MQ=255)
                                  ggTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTg                                                                                            >  1:449037/1‑139 (MQ=255)
                                    tCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGc                                                                                          <  2:449037/139‑1 (MQ=255)
                                       aaaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAAt                                                                                       <  1:1212259/139‑1 (MQ=255)
                                               tcaTGTAAATGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTCGCTGTTGGCAATCCTTGTTc                                                                               <  1:1106743/137‑1 (MQ=255)
                                               tGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACATCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc                                                                               <  2:1689090/139‑1 (MQ=255)
                                                    tAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa                                                                          >  1:1651728/1‑139 (MQ=255)
                                                     aaaaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaa                                                                         >  2:1067739/1‑139 (MQ=255)
                                                           cAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGc                                                                                          >  2:754736/1‑116 (MQ=255)
                                                           cAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGc                                                                                          <  1:754736/116‑1 (MQ=255)
                                                            aaaCGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             <  1:431132/80‑1 (MQ=255)
                                                            aaaCGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             >  2:431132/1‑80 (MQ=255)
                                                            aaaCGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGt                                                                                                          >  1:8252/1‑99 (MQ=255)
                                                            aaaCGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGt                                                                                                          <  2:8252/99‑1 (MQ=255)
                                                             aaCGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtagt                                                                 >  2:250219/1‑139 (MQ=255)
                                                               cgcgAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg                                                               <  1:250219/139‑1 (MQ=255)
                                                                    ggCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGt                                                          >  2:622018/1‑139 (MQ=255)
                                                                        aaGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATc                                                                                      >  1:1817853/1‑107 (MQ=255)
                                                                        aaGACCTCGCGTTTTGCGTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATc                                                                                      <  2:1817853/107‑1 (MQ=255)
                                                                              tCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                                >  1:155280/1‑107 (MQ=255)
                                                                              tCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                                <  2:155280/107‑1 (MQ=255)
                                                                              tCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGc                                                >  2:775361/1‑139 (MQ=255)
                                                                               cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAg                                                                                                                                     <  1:1818896/53‑1 (MQ=255)
                                                                               cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAg                                                                                                                                     >  2:1818896/1‑53 (MQ=255)
                                                                               cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             >  2:1616345/1‑61 (MQ=255)
                                                                               cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             <  1:1616345/61‑1 (MQ=255)
                                                                               cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGc                                                                                                 <  1:757121/89‑1 (MQ=255)
                                                                               cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGc                                                                                                 >  2:757121/1‑89 (MQ=255)
                                                                               cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                                >  2:1674545/1‑106 (MQ=255)
                                                                               cgcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                                <  1:1674545/106‑1 (MQ=255)
                                                                                gcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             >  2:1160149/1‑60 (MQ=255)
                                                                                gcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGa                                                                                                                             <  1:1160149/60‑1 (MQ=255)
                                                                                gcgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCAc                                              >  1:1241687/1‑139 (MQ=255)
                                                                                 cgTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACg                                             <  1:622018/139‑1 (MQ=255)
                                                                                                 aCCAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGa                             >  1:1868100/1‑139 (MQ=255)
                                                                                                  ccAGATGATATTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGAc                            <  2:1241687/139‑1 (MQ=255)
                                                                                                          tatTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                                <  2:1412279/79‑1 (MQ=255)
                                                                                                          tatTTTTCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                                >  1:1412279/1‑79 (MQ=255)
                                                                                                            tttttCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTgg                                                                                                  <  2:695623/59‑1 (MQ=255)
                                                                                                            tttttCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTgg                                                                                                  >  1:695623/1‑59 (MQ=255)
                                                                                                            tttttCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCa                                               <  1:1745411/110‑1 (MQ=255)
                                                                                                            tttttCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCa                                               >  2:1745411/1‑110 (MQ=255)
                                                                                                               ttCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGc                                                <  1:667878/106‑1 (MQ=255)
                                                                                                               ttCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGc                                                >  2:667878/1‑106 (MQ=255)
                                                                                                               ttCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCa               >  2:1869791/1‑139 (MQ=255)
                                                                                                                tCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTgg                                                                                           >  2:415337/1‑62 (MQ=255)
                                                                                                                tCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTgg                                                                                           <  1:415337/62‑1 (MQ=255)
                                                                                                                tCTGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCaa              >  2:1001500/1‑139 (MQ=255)
                                                                                                                  tGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACcgcg                                                                                                            >  2:1798435/1‑43 (MQ=255)
                                                                                                                  tGAAAGCACATGGACCAGGTGTTTGAACATATTAAACACcgcg                                                                                                            <  1:1798435/43‑1 (MQ=255)
                                                                                                                     aaGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCt         <  1:1869791/139‑1 (MQ=255)
                                                                                                                      aGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTg        <  1:775361/139‑1 (MQ=255)
                                                                                                                      aGCACATGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTg        <  1:1001500/139‑1 (MQ=255)
                                                                                                                           aTGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCAtt                                        >  1:765474/1‑102 (MQ=255)
                                                                                                                           aTGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCAtt                                        <  2:765474/102‑1 (MQ=255)
                                                                                                                            tGGACCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCg  <  1:646714/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                       
CGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCG  >  NC_000913/1330327‑1330589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A