Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,318,019 |
G→A |
R620C (CGT→TGT) |
rcsC ← |
hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,318,019 | 0 | G | A | 100.0%
| 106.7
/ NA
| 34 | R620C (CGT→TGT) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/16); total (18/16) |
TCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAC > NC_000913/2317884‑2318154
|
tCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGAt < 2:522361/139‑1 (MQ=255)
cGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCACACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg < 1:71219/139‑1 (MQ=255)
gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATagag > 1:103955/1‑111 (MQ=255)
gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATagag < 2:103955/111‑1 (MQ=255)
gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATaga < 2:521888/110‑1 (MQ=255)
gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATaga > 1:521888/1‑110 (MQ=255)
gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCa > 1:100346/1‑139 (MQ=255)
cTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACt > 1:1191756/1‑139 (MQ=255)
aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAg > 1:1118798/1‑139 (MQ=255)
aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAg < 2:1020666/139‑1 (MQ=255)
aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAg > 1:51538/1‑139 (MQ=255)
agGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa < 2:669474/139‑1 (MQ=255)
agGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa > 1:669474/1‑139 (MQ=255)
gggATCAAACAAGCACACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATagag < 1:1081741/67‑1 (MQ=255)
gggATCAAACAAGCACACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATagag > 2:1081741/1‑67 (MQ=255)
tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaa < 2:626076/93‑1 (MQ=255)
tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaa > 1:626076/1‑93 (MQ=255)
cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCgg < 1:1133819/111‑1 (MQ=255)
cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCgg > 2:1133819/1‑111 (MQ=255)
cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGa > 1:522155/1‑139 (MQ=255)
cttTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATgg < 1:1187500/105‑1 (MQ=255)
cttTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATgg > 2:1187500/1‑105 (MQ=255)
tCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGAc > 1:1082678/1‑127 (MQ=255)
tCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGAc < 2:1082678/127‑1 (MQ=255)
tCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACg > 1:1215251/1‑139 (MQ=255)
gCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCa > 2:600649/1‑139 (MQ=255)
aTCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt < 2:1191756/139‑1 (MQ=255)
ccccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCg < 2:1215251/139‑1 (MQ=255)
gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCAGCTGTAAACGCATCGGGTCGCCAttt > 1:344638/1‑139 (MQ=255)
ccGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg < 2:756322/50‑1 (MQ=255)
ccGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg > 1:756322/1‑50 (MQ=255)
aaCACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTa < 2:1017881/128‑1 (MQ=255)
aaCACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTa > 1:1017881/1‑128 (MQ=255)
aaCACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGc < 2:51538/139‑1 (MQ=255)
cacaGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcac > 2:686693/1‑139 (MQ=255)
|
TCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAC > NC_000913/2317884‑2318154
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
