Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,318,019	G→A	R620C (CGT→TGT)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,318,019	0	G	A	100.0%	160.3 / NA	50	R620C (CGT→TGT)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/22); total (28/22)

TTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTG  >  NC_000913/2317888‑2318150

tttttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATagag                                                                                                                              >  2:529149/1‑139 (MQ=255)

                     cagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                         <  2:1234124/139‑1 (MQ=255)

                     cagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                         <  1:735668/139‑1 (MQ=255)

                      agaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGaa                                                                                                        >  1:1019707/1‑139 (MQ=255)

                       gaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAAc                                                                                                       >  2:1427743/1‑139 (MQ=255)

                          cGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                    <  2:1430612/139‑1 (MQ=255)

                             tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGa                                                                                                                                   >  2:681972/1‑105 (MQ=255)

                             tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaa                                                                                                 <  1:1427743/139‑1 (MQ=255)

                                        ttACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGc                                                                                      <  1:529149/139‑1 (MQ=255)

                                                        ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                    >  1:1086444/1‑109 (MQ=255)

                                                        ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                    <  2:1086444/109‑1 (MQ=255)

                                                        ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt                                                                      >  1:224595/1‑139 (MQ=255)

                                                          ccGACCTGTAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc                                                                                                                   >  2:1414253/1‑92 (MQ=255)

                                                          ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc                                                                                                                   <  1:1414253/92‑1 (MQ=255)

                                                          ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTa                                                                    >  1:36460/1‑139 (MQ=255)

                                                                  gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGt                                                                                  >  2:91117/1‑117 (MQ=255)

                                                                  gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGt                                                                                  <  1:91117/117‑1 (MQ=255)

                                                                       agGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                       <  2:1019707/139‑1 (MQ=255)

                                                                        gggATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAAc                                                      >  2:360273/1‑139 (MQ=255)

                                                                            tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATATAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGt                                                  >  1:1403772/1‑139 (MQ=255)

                                                                             cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGtt                                                 >  1:303196/1‑139 (MQ=255)

                                                                                 cAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATATAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGgag                                             <  2:1403772/139‑1 (MQ=255)

                                                                                 cAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGgag                                             <  2:36460/139‑1 (MQ=255)

                                                                                   aGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGAt                                           >  2:1347953/1‑139 (MQ=255)

                                                                                     cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAgagaga                                                                                                                           <  2:1192235/57‑1 (MQ=255)

                                                                                     cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAgagaga                                                                                                                           >  1:1192235/1‑57 (MQ=255)

                                                                                     cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                            >  2:338222/1‑72 (MQ=255)

                                                                                     cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                            <  1:338222/72‑1 (MQ=255)

                                                                                     cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCg                                                               <  2:483955/117‑1 (MQ=255)

                                                                                     cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCg                                                               >  1:483955/1‑117 (MQ=255)

                                                                                          cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCa                                                                                                                  <  1:1278638/61‑1 (MQ=255)

                                                                                          cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCa                                                                                                                  >  2:1278638/1‑61 (MQ=255)

                                                                                                       ggTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCACCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCg                       >  1:359036/1‑139 (MQ=255)

                                                                                                          aTCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                    <  2:224595/139‑1 (MQ=255)

                                                                                                           tCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTc                   <  2:303196/139‑1 (MQ=255)

                                                                                                             cccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGc                 <  1:360273/139‑1 (MQ=255)

                                                                                                               cACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCa               >  2:974142/1‑139 (MQ=255)

                                                                                                                aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCAt              >  1:284271/1‑139 (MQ=255)

                                                                                                                      gTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTa                                                                    >  1:797781/1‑79 (MQ=255)

                                                                                                                      gTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTa                                                                    <  2:797781/79‑1 (MQ=255)

                                                                                                                       tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                    <  1:64980/46‑1 (MQ=255)

                                                                                                                       tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                    >  2:64980/1‑46 (MQ=255)

                                                                                                                       tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGc       >  2:471646/1‑139 (MQ=255)

                                                                                                                       tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGc       >  2:653953/1‑139 (MQ=255)

                                                                                                                       tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGc       >  1:1370579/1‑139 (MQ=255)

                                                                                                                            cgAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGcc      >  1:812069/1‑135 (MQ=255)

                                                                                                                            cgAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGcc      <  2:812069/135‑1 (MQ=255)

                                                                                                                            cgAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTg  >  2:1038760/1‑139 (MQ=255)

                                                                                                                              aaCACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCgg                      >  2:437757/1‑117 (MQ=255)

                                                                                                                              aaCACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCgg                      <  1:437757/117‑1 (MQ=255)

TTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTG  >  NC_000913/2317888‑2318150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A