Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 770 68.5 3470224 91.7% 3182195 103.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,019 G→A R620C (CGT→TGT)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0190GA100.0% 237.4 / NA 73R620C (CGT→TGT) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/37);  total (36/37)

ATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCA  >  NC_000913/2317898‑2318147
                                                                                                                          |                                                                                                                                
aTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGa                                                                                                                                >  2:1643272/1‑124 (MQ=255)
        acccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                         <  1:1077678/139‑1 (MQ=255)
                  gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGc                                                                                                >  2:345009/1‑139 (MQ=255)
                  gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACa                                                                                                   <  2:1231068/135‑1 (MQ=255)
                  gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACa                                                                                                   >  1:1231068/1‑135 (MQ=255)
                   tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAAt                                                                                                                    <  2:707258/117‑1 (MQ=255)
                   tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAAt                                                                                                                    >  1:707258/1‑117 (MQ=255)
                         ggAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtat                                                                                        <  2:1200370/139‑1 (MQ=255)
                         ggAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtat                                                                                        <  1:276013/139‑1 (MQ=255)
                             aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCAGCCGCGCGAACATGCAAAACTATACAg                                                                                    >  2:1102427/1‑139 (MQ=255)
                                    tGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                         >  2:152659/1‑111 (MQ=255)
                                    tGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                         <  1:152659/111‑1 (MQ=255)
                                                ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgc                                                                                                          >  1:177292/1‑98 (MQ=255)
                                                ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgc                                                                                                          <  2:177292/98‑1 (MQ=255)
                                                ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTa                                                                 >  2:1444213/1‑139 (MQ=255)
                                                ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTa                                                                 >  2:261803/1‑139 (MQ=255)
                                                     cTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCg                                                            <  1:1444213/139‑1 (MQ=255)
                                                        gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 >  2:497919/1‑99 (MQ=255)
                                                        gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 <  1:497919/99‑1 (MQ=255)
                                                              gggATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAAc                                                   >  1:165549/1‑139 (MQ=255)
                                                                gATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt                                                                   <  2:641447/121‑1 (MQ=255)
                                                                gATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt                                                                   >  1:641447/1‑121 (MQ=255)
                                                                  tCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGt                                               >  1:1307941/1‑139 (MQ=255)
                                                                         aGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATTGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                      <  2:74099/77‑1 (MQ=255)
                                                                         aGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATTGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                      >  1:74099/1‑77 (MQ=255)
                                                                          gcgcACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATaga                                                                                                                            <  1:647666/54‑1 (MQ=255)
                                                                          gcgcACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATaga                                                                                                                            >  2:647666/1‑54 (MQ=255)
                                                                           cgcACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATagag                                                                                                                           <  1:174786/54‑1 (MQ=255)
                                                                           cgcACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATagag                                                                                                                           >  2:174786/1‑54 (MQ=255)
                                                                           cgcACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGa                                      >  2:303188/1‑139 (MQ=255)
                                                                           cgcACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGa                                      >  1:836498/1‑139 (MQ=255)
                                                                           cgcACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGa                                      >  2:48899/1‑139 (MQ=255)
                                                                            gcACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGAc                                     <  2:165549/139‑1 (MQ=255)
                                                                                cacTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACa                                                  <  1:469573/122‑1 (MQ=255)
                                                                                cacTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACa                                                  >  2:469573/1‑122 (MQ=255)
                                                                                cacTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACctgc                                 <  2:1053381/139‑1 (MQ=255)
                                                                                cacTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACctgc                                 <  1:303188/139‑1 (MQ=255)
                                                                                 acTTCTTTCGCCGGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACctgc                                 <  1:345009/139‑1 (MQ=255)
                                                                                  cttcttTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCg                                                                           >  1:600065/1‑95 (MQ=255)
                                                                                  cttcttTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCg                                                                           <  2:600065/95‑1 (MQ=255)
                                                                                  cttcttTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACctgctg                               >  1:215040/1‑139 (MQ=255)
                                                                                     cttTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGt                                                           >  1:913142/1‑108 (MQ=255)
                                                                                     cttTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGt                                                           <  2:913142/108‑1 (MQ=255)
                                                                                        tCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATgg                                                              <  2:996094/102‑1 (MQ=255)
                                                                                        tCGCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATgg                                                              >  1:996094/1‑102 (MQ=255)
                                                                                          gCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 <  2:116552/65‑1 (MQ=255)
                                                                                          gCC‑GGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 >  1:116552/1‑65 (MQ=255)
                                                                                           cc‑GGTATCCCCACGCCGGTATCGCGAACACAGATaga                                                                                                                            <  2:1408128/37‑1 (MQ=255)
                                                                                           cc‑GGTATCCCCACGCCGGTATCGCGAACACAGATaga                                                                                                                            >  1:1408128/1‑37 (MQ=255)
                                                                                                tATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCggg                  >  2:332167/1‑139 (MQ=255)
                                                                                                 aTCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                 <  2:836498/139‑1 (MQ=255)
                                                                                                   ccccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCg               <  1:111722/139‑1 (MQ=255)
                                                                                                   ccccACGCCGGTATCGCGAACACAGATAGAGAGATAAGCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCg               <  2:567793/139‑1 (MQ=255)
                                                                                                       aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                    <  1:219133/98‑1 (MQ=255)
                                                                                                       aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                    >  2:219133/1‑98 (MQ=255)
                                                                                                         gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGgaga                                         >  2:381126/1‑107 (MQ=255)
                                                                                                         gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGgaga                                         <  1:381126/107‑1 (MQ=255)
                                                                                                         gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCg                    <  2:812339/128‑1 (MQ=255)
                                                                                                         gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCg                    >  1:812339/1‑128 (MQ=255)
                                                                                                           cGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTaa       <  2:215040/139‑1 (MQ=255)
                                                                                                             gTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCAt                      <  2:347256/122‑1 (MQ=255)
                                                                                                             gTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCAt                      >  1:347256/1‑122 (MQ=255)
                                                                                                              tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGc                                                                                   <  1:582917/60‑1 (MQ=255)
                                                                                                              tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGc                                                                                   >  2:582917/1‑60 (MQ=255)
                                                                                                              tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCg                                                                           >  1:436743/1‑68 (MQ=255)
                                                                                                              tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCg                                                                           <  2:436743/68‑1 (MQ=255)
                                                                                                              tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGc    >  2:1235271/1‑139 (MQ=255)
                                                                                                                tCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa  <  1:332167/139‑1 (MQ=255)
                                                                                                                tCGCGAACACAGATAGAGAGATAATCGCCAGCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTCTGGCGTTACTCAACATGTTGGAGATGTCCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa  <  1:1102427/139‑1 (MQ=255)
                                                                                                                   cgAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 >  2:739151/1‑41 (MQ=255)
                                                                                                                   cgAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 <  1:739151/41‑1 (MQ=255)
                                                                                                                     aaCACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGc              <  1:145454/122‑1 (MQ=255)
                                                                                                                     aaCACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGc              >  2:145454/1‑122 (MQ=255)
                                                                                                                          |                                                                                                                                
ATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCC‑GGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCA  >  NC_000913/2317898‑2318147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A