Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,597,904 |
G→A |
P321L (CCG→CTG) |
bamC ← |
BamABCDE complex OM biogenesis lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,597,904 | 0 | G | A | 97.8%
| 140.9
/ NA
| 46 | P321L (CCG→CTG) | bamC | BamABCDE complex OM biogenesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (20/25); total (21/25) |
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACG > NC_000913/2597769‑2598035
|
cAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGAt < 2:478844/139‑1 (MQ=255)
aCGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGa > 2:903220/1‑139 (MQ=255)
cGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGAt < 2:211958/139‑1 (MQ=255)
gCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATg < 1:6256/139‑1 (MQ=255)
gCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGgc < 1:310313/139‑1 (MQ=255)
atcatatcaGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGgctgct > 1:985640/1‑139 (MQ=255)
aGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAGTCTGAGTCAGAGTATGACCTTTCGGATCGATGAATTGTAGGCTGCTGCGGTTATCTAAATc > 1:775696/1‑139 (MQ=255)
ccGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCa < 1:903220/139‑1 (MQ=255)
tgatTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg < 1:129106/139‑1 (MQ=255)
ttCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGAt < 2:926966/97‑1 (MQ=255)
ttCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGAt > 1:926966/1‑97 (MQ=255)
tCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGt > 2:442167/1‑139 (MQ=255)
cccTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtata > 2:510116/1‑139 (MQ=255)
tATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGAt > 2:678425/1‑82 (MQ=255)
tATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGAt < 1:678425/82‑1 (MQ=255)
aCTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 2:985640/139‑1 (MQ=255)
aCTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 1:408299/139‑1 (MQ=255)
aCTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTCAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTCCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 2:775696/139‑1 (MQ=255)
tGCTAAACGCAGCCTGGATGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGcc > 2:802090/1‑109 (MQ=255)
tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGgctgc < 1:730195/90‑1 (MQ=255)
tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGgctgc > 2:730195/1‑90 (MQ=255)
tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGcc < 1:802090/109‑1 (MQ=255)
tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 1:1089929/136‑1 (MQ=255)
tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc > 2:1089929/1‑136 (MQ=255)
tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAg > 1:549919/1‑139 (MQ=255)
aGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGcc < 1:442167/139‑1 (MQ=255)
aGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg > 1:379731/1‑93 (MQ=255)
aGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg < 2:379731/93‑1 (MQ=255)
cgcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGcc > 1:360378/1‑126 (MQ=255)
cgcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGcc < 2:360378/126‑1 (MQ=255)
gcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc > 1:585164/1‑104 (MQ=255)
gcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 2:585164/104‑1 (MQ=255)
tGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCg < 2:402551/139‑1 (MQ=255)
gAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCgg > 1:378326/1‑139 (MQ=255)
aGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGc > 2:203930/1‑139 (MQ=255)
tGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGt < 2:508608/67‑1 (MQ=255)
tGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGt > 1:508608/1‑67 (MQ=255)
tGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAg < 1:1034275/125‑1 (MQ=255)
tGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAg > 2:1034275/1‑125 (MQ=255)
gACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg < 1:1159805/55‑1 (MQ=255)
gACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg > 2:1159805/1‑55 (MQ=255)
aCCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 2:86726/75‑1 (MQ=255)
aCCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc > 1:86726/1‑75 (MQ=255)
aCCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACg > 2:255422/1‑139 (MQ=255)
ttCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCt < 2:298669/49‑1 (MQ=255)
ttCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 1:674140/71‑1 (MQ=255)
|
CAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACG > NC_000913/2597769‑2598035
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
