Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,685,561	T→C	intergenic (‑54/‑274)	glyA ← / → hmp	serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,685,561	0	T	C	100.0%	133.7 / NA	37	intergenic (‑54/‑274)	glyA/hmp	serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/23); total (14/23)

GACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCTGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGAT  >  NC_000913/2685432‑2685692

gACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACaa                                                                                                                            >  2:97457/1‑139 (MQ=255)

       tttttCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                     <  1:196034/139‑1 (MQ=255)

       tttttCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                     <  2:937864/139‑1 (MQ=255)

       tttttCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                     <  1:307248/139‑1 (MQ=255)

       tttttCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                     <  1:370441/139‑1 (MQ=255)

             cTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGtat                                                                                                               <  1:209281/139‑1 (MQ=255)

             cTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGtat                                                                                                               <  1:683208/139‑1 (MQ=255)

               gCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATaa                                                                                                             <  2:430342/139‑1 (MQ=255)

                        ccTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCAt                                                                                                    <  1:817024/139‑1 (MQ=255)

                             cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                     <  1:1188802/117‑1 (MQ=255)

                             cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                     >  2:1188802/1‑117 (MQ=255)

                                       catcatAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGt                                                                                     <  1:97457/139‑1 (MQ=255)

                                            tAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgt                                                                                                 >  2:835144/1‑122 (MQ=255)

                                            tAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgt                                                                                                 <  1:835144/122‑1 (MQ=255)

                                            tAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAAc                                                                                         <  2:290580/130‑1 (MQ=255)

                                            tAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAAc                                                                                         >  1:290580/1‑130 (MQ=255)

                                                      tGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                          >  1:1132907/1‑119 (MQ=255)

                                                      tGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                          <  2:1132907/119‑1 (MQ=255)

                                                                  gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                          >  2:653621/1‑139 (MQ=255)

                                                                  gCTTTAACATCCGCAGCTCCTGAGTCAGCTAACAATAAAGTTTTTTGCCTTTATAGGTGGTCCCGTTGGACAACGGCGAACCGTATAACCGAATGAATGTGCGATAACAAGTCTTGACACAGGAATTTACGCAAACgtt                                                          <  1:267466/139‑3 (MQ=255)

                                                                            ccGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAgg                                                <  1:653621/139‑1 (MQ=255)

                                                                                   tcCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                          <  1:419249/122‑1 (MQ=255)

                                                                                   tcCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                          >  2:419249/1‑122 (MQ=255)

                                                                                            aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                     <  2:94912/54‑1 (MQ=255)

                                                                                            aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                     >  1:94912/1‑54 (MQ=255)

                                                                                            aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTgg                                >  2:984481/1‑139 (MQ=255)

                                                                                              cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                          <  2:385480/79‑1 (MQ=255)

                                                                                              cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                          >  1:385480/1‑79 (MQ=255)

                                                                                              cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                          >  2:282917/1‑111 (MQ=255)

                                                                                              cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                          <  1:282917/111‑1 (MQ=255)

                                                                                               tAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCAt                                                                                                    <  2:295048/68‑1 (MQ=255)

                                                                                               tAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCAt                                                                                                    >  1:295048/1‑68 (MQ=255)

                                                                                                  cAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCttt                                  >  2:19481/1‑131 (MQ=255)

                                                                                                  cAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCttt                                  <  1:19481/131‑1 (MQ=255)

                                                                                                                          gCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGAt  <  2:318426/139‑1 (MQ=255)

                                                                                                                             gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGGGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTgg                                <  2:322988/106‑1 (MQ=255)

                                                                                                                             gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGGGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTgg                                >  1:322988/1‑106 (MQ=255)

GACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCTGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGAT  >  NC_000913/2685432‑2685692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A