Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I2 R1 760 52.7 2744488 90.2% 2475528 105.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,685,561 T→C intergenic (‑54/‑274) glyA ← / → hmp serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,685,5610TC100.0% 230.4 / NA 64intergenic (‑54/‑274)glyA/hmpserine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/35);  total (29/35)

CGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCTGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTCT  >  NC_000913/2685434‑2685696
                                                                                                                               |                                                                                                                                       
cGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACg                                                                                                                              >  1:373539/1‑139 (MQ=255)
   aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           <  1:1296501/139‑1 (MQ=255)
   aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           <  1:1364139/139‑1 (MQ=255)
   aCTTTTTCCTGCTCCATAGCCTCCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACACGTTAACATCCGCATCTCCTGACTCCTCTAACAATAAAATTTTTGGCCTTTGTAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           <  1:414284/139‑1 (MQ=255)
             gCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATaa                                                                                                                 >  2:136252/1‑139 (MQ=255)
             gCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATaa                                                                                                                 >  2:80998/1‑139 (MQ=255)
                  aTAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGaa                                                                                                            <  2:722791/139‑1 (MQ=255)
                     gCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCa                                                                                                         <  2:373539/139‑1 (MQ=255)
                      ccTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCAt                                                                                                        <  1:988273/139‑1 (MQ=255)
                        tGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtg                                                                                                      <  1:1046962/139‑1 (MQ=255)
                         gCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                         >  2:1284194/1‑119 (MQ=255)
                         gCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                         <  1:1284194/119‑1 (MQ=255)
                           cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGc                                                                                                   >  1:379197/1‑139 (MQ=255)
                                ttCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              <  2:60501/139‑1 (MQ=255)
                                 tCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAAc                                                                                             <  2:379197/139‑1 (MQ=255)
                                    gCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAgg                                                                                          >  2:978736/1‑139 (MQ=255)
                                     catcatAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGt                                                                                                                     <  2:1045256/111‑1 (MQ=255)
                                     catcatAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGt                                                                                                                     >  1:1045256/1‑111 (MQ=255)
                                     catcatAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGt                                                                                         >  2:55348/1‑139 (MQ=255)
                                        catAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCtt                                                                                      <  1:1284323/139‑1 (MQ=255)
                                        catAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCtt                                                                                      >  1:1269291/1‑139 (MQ=255)
                                                  aaTGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGaa                                                                            >  1:519214/1‑139 (MQ=255)
                                                   aTGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           <  1:1279043/91‑1 (MQ=255)
                                                   aTGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           >  2:1279043/1‑91 (MQ=255)
                                                    tGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAAtt                                                                          >  1:803457/1‑139 (MQ=255)
                                                        cATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACg                                                                      <  2:773366/139‑1 (MQ=255)
                                                         aTTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGc                                                                     <  1:55348/139‑1 (MQ=255)
                                                          tttCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCa                                                                    <  1:80998/139‑1 (MQ=255)
                                                           ttCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCaa                                                                   <  1:136252/139‑1 (MQ=255)
                                                                gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              >  1:301443/1‑139 (MQ=255)
                                                                gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              >  2:22534/1‑139 (MQ=255)
                                                                 cTTTAACATCCGCATCTACTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTTTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAtg                                                             <  2:839527/139‑2 (MQ=255)
                                                                      aCATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCtt                                                        <  2:301443/139‑1 (MQ=255)
                                                                          ccGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              >  2:453862/1‑129 (MQ=255)
                                                                          ccGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              <  1:453862/129‑1 (MQ=255)
                                                                            gCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGa                                                                                                                                   >  1:1019148/1‑58 (MQ=255)
                                                                            gCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGa                                                                                                                                   <  2:1019148/58‑1 (MQ=255)
                                                                            gCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           >  2:70767/1‑66 (MQ=255)
                                                                            gCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           <  1:70767/66‑1 (MQ=255)
                                                                            gCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTc                                                       <  2:3633/134‑1 (MQ=255)
                                                                            gCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTc                                                       >  1:3633/1‑134 (MQ=255)
                                                                            gCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCt                                                  >  2:612838/1‑139 (MQ=255)
                                                                               tctcCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACg                                               <  1:733405/139‑1 (MQ=255)
                                                                                 tcCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCa                                             <  1:22534/139‑1 (MQ=255)
                                                                                     gACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgtg                                                                                                    >  2:89246/1‑80 (MQ=255)
                                                                                     gACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgtg                                                                                                    <  1:89246/80‑1 (MQ=255)
                                                                                     gACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACaa                                                                                 >  1:1011286/1‑99 (MQ=255)
                                                                                     gACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACaa                                                                                 <  2:1011286/99‑1 (MQ=255)
                                                                                      aCTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCtt                                                        >  2:510714/1‑123 (MQ=255)
                                                                                      aCTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCtt                                                        <  1:510714/123‑1 (MQ=255)
                                                                                        tCAGCTAACAATAAAATTTTTCGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCttt                                      <  2:803457/139‑1 (MQ=255)
                                                                                          aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           >  1:9356/1‑52 (MQ=255)
                                                                                          aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           <  2:9356/52‑1 (MQ=255)
                                                                                          aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGtat                                                                                                                   <  1:1072168/60‑1 (MQ=255)
                                                                                          aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGtat                                                                                                                   >  2:1072168/1‑60 (MQ=255)
                                                                                           gCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGt                                                                                                                     >  2:491859/1‑57 (MQ=255)
                                                                                           gCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGt                                                                                                                     <  1:491859/57‑1 (MQ=255)
                                                                                                cAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              >  2:1236260/1‑75 (MQ=255)
                                                                                                cAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              <  1:1236260/75‑1 (MQ=255)
                                                                                                  aTAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGtat                                                                                                                   >  1:812625/1‑52 (MQ=255)
                                                                                                  aTAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGtat                                                                                                                   <  2:812625/52‑1 (MQ=255)
                                                                                                                   tataGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              >  1:1098920/1‑88 (MQ=255)
                                                                                                                   tataGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              <  2:1098920/88‑1 (MQ=255)
                                                                                                                            tCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTCt  >  1:586120/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                       
CGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCTGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTCT  >  NC_000913/2685434‑2685696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A