Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A12 F1 I1 R1 | 760 | 58.2 | 3022526 | 90.8% | 2744453 | 102.0 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,685,561 | T→C | intergenic (‑54/‑274) | glyA ← / → hmp | serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,685,561 | 0 | T | C | 100.0% | 223.5 / NA | 61 | intergenic (‑54/‑274) | glyA/hmp | serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/30); total (31/30) | |||||||||||
TGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCTGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTC > NC_000913/2685431‑2685695 | tGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACa < 2:1397193/139‑1 (MQ=255) gACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACac > 2:290164/1‑138 (MQ=255) cGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACg < 1:1376563/139‑1 (MQ=255) aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg > 1:757770/1‑139 (MQ=255) aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg < 1:290164/139‑1 (MQ=255) aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg < 2:973628/139‑1 (MQ=255) tAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGaa < 1:28071/139‑1 (MQ=255) tAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAAt > 1:1126157/1‑139 (MQ=255) gCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgt > 2:1330919/1‑139 (MQ=255) cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGc > 1:543133/1‑139 (MQ=255) cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGc > 2:200887/1‑139 (MQ=255) cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTAACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGc > 1:1349524/1‑139 (MQ=255) ttCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg < 1:1140193/110‑1 (MQ=255) ttCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg > 2:1140193/1‑110 (MQ=255) ttCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa > 2:881289/1‑139 (MQ=255) ttCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa < 1:1330919/139‑1 (MQ=255) tCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAAc < 2:543133/139‑1 (MQ=255) catcatAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGt > 2:284952/1‑139 (MQ=255) catAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCtt < 1:962421/139‑1 (MQ=255) aaTCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGAc < 2:757770/139‑1 (MQ=255) tGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGa > 1:1090915/1‑82 (MQ=255) tGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGa < 2:1090915/82‑1 (MQ=255) aCGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACg > 2:944849/1‑77 (MQ=255) aCGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACg < 1:944849/77‑1 (MQ=255) aCGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACg > 1:913391/1‑139 (MQ=255) gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCg < 2:1382418/103‑1 (MQ=255) gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCg > 1:1382418/1‑103 (MQ=255) aaCATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg > 2:1241802/1‑73 (MQ=255) aaCATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg < 1:1241802/73‑1 (MQ=255) cATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTc < 1:881289/139‑1 (MQ=255) ccGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAgg < 1:200887/139‑1 (MQ=255) ccTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa < 1:749718/89‑1 (MQ=255) ccTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa > 2:749718/1‑89 (MQ=255) tGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGa < 2:288198/59‑1 (MQ=255) tGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGa > 1:288198/1‑59 (MQ=255) aGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTgg > 1:572134/1‑139 (MQ=255) aGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTgg > 2:258887/1‑139 (MQ=255) cTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg > 1:188201/1‑50 (MQ=255) cTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCg < 2:188201/50‑1 (MQ=255) cTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACg < 1:722634/103‑1 (MQ=255) cTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACg > 2:722634/1‑103 (MQ=255) cTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCttt > 2:1041549/1‑135 (MQ=255) cTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCttt < 1:1041549/135‑1 (MQ=255) cTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCt > 2:572139/1‑133 (MQ=255) cTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCt < 1:572139/133‑1 (MQ=255) aaCAATAAAATTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGaa < 2:1349524/139‑1 (MQ=255) aaTTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgtg > 1:446469/1‑63 (MQ=255) aaTTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgtg < 2:446469/63‑1 (MQ=255) aaTTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa < 2:934350/69‑1 (MQ=255) aaTTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa > 1:934350/1‑69 (MQ=255) aTTTTTGGCC‑TTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCt < 1:258887/139‑1 (MQ=255) gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTATGCAAACGAt > 2:1124902/1‑80 (MQ=255) gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTATGCAAACGAt < 1:1124902/80‑1 (MQ=255) gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCaaa < 2:1442308/76‑1 (MQ=255) gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCaaa > 1:1442308/1‑76 (MQ=255) gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATaa < 1:1089661/113‑1 (MQ=255) gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATaa > 2:1089661/1‑113 (MQ=255) gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTc > 2:1417935/1‑139 (MQ=255) gTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTc > 1:647841/1‑139 (MQ=255) tCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgtg < 2:756364/41‑1 (MQ=255) tCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAgg > 1:284172/1‑89 (MQ=255) | TGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCC‑TTTATAGGCGGTCCTGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTC > NC_000913/2685431‑2685695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |