Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,347,945	T→C	R277R (CGT→CGC)	yhbJ →	adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,347,945	0	T	C	100.0%	232.6 / NA	64	R277R (CGT→CGC)	yhbJ	adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/31); total (33/31)

TGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTAC  >  NC_000913/3347809‑3348074

tGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCAc                                                                                                                                 >  1:1035424/1‑139 (MQ=255)

    ggAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGGAGCACCGTTCGGGGTATATTGCAGAGCAACTGGCAGACGCCTTACGCTCGCGCGGTAAAAACGACCAGTCACGCCATCGCAAGCTg                                                                                                                             >  1:1092154/1‑139 (MQ=255)

     gAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTgg                                                                                                                            >  1:1003804/1‑139 (MQ=255)

      aaaCCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGa                                                                                                                           <  2:1035424/139‑1 (MQ=255)

       aaCCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGaa                                                                                                                          >  2:60245/1‑139 (MQ=255)

           aacaacCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAAc                                                                                                                      >  1:349766/1‑139 (MQ=255)

            acaacCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACg                                                                                                                     <  2:349766/139‑1 (MQ=255)

                   tAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCa                                                                                                              >  2:227750/1‑139 (MQ=255)

                   tAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCa                                                                                                              >  1:1165298/1‑139 (MQ=255)

                    aGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCAt                                                                                                             >  2:377679/1‑139 (MQ=255)

                    aGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCAt                                                                                                             <  2:1022160/139‑1 (MQ=255)

                    aGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCAt                                                                                                             >  2:140024/1‑139 (MQ=255)

                        aCTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTTGAAAAACGTAAACCATGAcc                                                                                                         >  1:937563/1‑139 (MQ=255)

                         cTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCg                                                                                                        <  2:643893/139‑1 (MQ=255)

                                   gCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAAc                                                                                              >  2:869590/1‑139 (MQ=255)

                                               aCCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATcac                                                                                  >  2:113209/1‑139 (MQ=255)

                                                  ggcggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa                                                                               <  1:1219723/139‑1 (MQ=255)

                                                    cggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAAt                                                                                     <  2:379606/131‑1 (MQ=255)

                                                    cggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAAt                                                                                     >  1:379606/1‑131 (MQ=255)

                                                    cggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaaca                                                                             >  1:1208286/1‑139 (MQ=255)

                                                          gCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg                                                                       <  1:869590/139‑1 (MQ=255)

                                                          gCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg                                                                       <  1:113209/139‑1 (MQ=255)

                                                           cACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg                                                                      <  1:377679/139‑1 (MQ=255)

                                                              cGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcat                                                                   >  1:1098758/1‑139 (MQ=255)

                                                               gTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  <  2:1098758/139‑1 (MQ=255)

                                                               gTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  <  2:1003804/139‑1 (MQ=255)

                                                                    gtgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCatgcatgc                                                             <  1:1029257/139‑1 (MQ=255)

                                                                       tataTTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGccca                                                          <  1:227750/139‑2 (MQ=255)

                                                                            tGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAATAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTg                                                     >  1:1007744/1‑139 (MQ=255)

                                                                                 agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  >  2:343248/1‑121 (MQ=255)

                                                                                 agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  <  1:343248/121‑1 (MQ=255)

                                                                                 agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                                                >  1:686936/1‑139 (MQ=255)

                                                                                 agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                                                >  2:110340/1‑139 (MQ=255)

                                                                                 agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                                                >  1:500656/1‑139 (MQ=255)

                                                                                   cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGtt                                                                                          >  1:1015184/1‑95 (MQ=255)

                                                                                   cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGtt                                                                                          <  2:1015184/95‑1 (MQ=255)

                                                                                   cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  <  2:948056/119‑1 (MQ=255)

                                                                                   cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  >  1:948056/1‑119 (MQ=255)

                                                                                    aaCTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAg                                             <  1:140024/139‑1 (MQ=255)

                                                                                    aaCTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAg                                             <  1:60245/139‑1 (MQ=255)

                                                                                      cTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCt                                           >  1:721189/1‑139 (MQ=255)

                                                                                             actactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGaa                                    >  1:180698/1‑139 (MQ=255)

                                                                                             actactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGaa                                    <  2:721189/139‑1 (MQ=255)

                                                                                                   tCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATg                              >  2:50191/1‑139 (MQ=255)

                                                                                                    ccGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGc                             >  1:892815/1‑139 (MQ=255)

                                                                                                         cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGaaa                                                                                                                         >  1:1051931/1‑42 (MQ=255)

                                                                                                         cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGaaa                                                                                                                         <  2:1051931/42‑1 (MQ=255)

                                                                                                         cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAGCCATGACCGTCAAGCa                                                                                                 >  2:485471/1‑66 (MQ=255)

                                                                                                         cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAGCCATGACCGTCAAGCa                                                                                                 <  1:485471/66‑1 (MQ=255)

                                                                                                         cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                                           <  1:189333/88‑1 (MQ=255)

                                                                                                         cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                                           >  2:189333/1‑88 (MQ=255)

                                                                                                         cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGt                        >  1:1070699/1‑139 (MQ=255)

                                                                                                           cgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                                           <  1:484070/86‑1 (MQ=255)

                                                                                                           cgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                                           >  2:484070/1‑86 (MQ=255)

                                                                                                           cgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGttt                      <  2:500656/139‑1 (MQ=255)

                                                                                                           cgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGttt                      <  1:254212/139‑1 (MQ=255)

                                                                                                                    aaCGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGa             >  2:1162576/1‑139 (MQ=255)

                                                                                                                     aCGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGaa            <  1:185820/139‑1 (MQ=255)

                                                                                                                        tCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTg         <  2:1070699/139‑1 (MQ=255)

                                                                                                                          cAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGaa            >  1:128494/1‑134 (MQ=255)

                                                                                                                          cAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGaa            <  2:128494/134‑1 (MQ=255)

                                                                                                                            gTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTg                                                                                         >  2:360774/1‑55 (MQ=255)

                                                                                                                            gTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTg                                                                                         <  1:360774/55‑1 (MQ=255)

                                                                                                                            gTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGctct     <  2:1165298/139‑1 (MQ=255)

                                                                                                                               aCGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTAc  <  1:50191/139‑1 (MQ=255)

TGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTAC  >  NC_000913/3347809‑3348074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A