Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,410,406	T→C	S43P (TCT→CCT)	dusB →	tRNA‑dihydrouridine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,410,406	0	T	C	100.0%	168.4 / NA	47	S43P (TCT→CCT)	dusB	tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/25); total (22/25)

TCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTG  >  NC_000913/3410271‑3410540

tCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGcctt                                                                                                                                     <  1:779258/139‑1 (MQ=255)

  ttAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCACTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttct                                                                                                                                   >  1:17241/1‑139 (MQ=255)

       tCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAAccc                                                                                                                              <  1:898093/139‑1 (MQ=255)

        cATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca                                                                                                                             <  2:96771/139‑1 (MQ=255)

        cATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca                                                                                                                             <  2:162522/139‑1 (MQ=255)

        cATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca                                                                                                                             <  2:29171/139‑1 (MQ=255)

                             gCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACaaa                                                                                                        >  2:848467/1‑139 (MQ=255)

                              cTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt                                                                                                       >  2:606188/1‑139 (MQ=255)

                               tCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtc                                                                                                      <  1:1032538/139‑1 (MQ=255)

                                cAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtct                                                                                                     >  2:8586/1‑139 (MQ=255)

                                  gAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCg                                                                                                   >  2:974746/1‑139 (MQ=255)

                                  gAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCg                                                                                                   >  2:1135406/1‑139 (MQ=255)

                                  gAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAATATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCg                                                                                                   >  1:851655/1‑139 (MQ=255)

                                   aaaTCGCCTGATCGTAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGTGCCGGATTGACAGTATCCGAGATGATCCCTTCTAACCCACAGGTTTGGGaaa                                                                                                                >  2:741077/1‑125 (MQ=255)

                                   aaaTCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGaaa                                                                                                                <  1:741077/125‑1 (MQ=255)

                                     aTCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGTTTTGGGAAAGCGACAAATCTCGttt                                                                                                <  1:128379/139‑1 (MQ=255)

                                       cGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTAc                                                                                              <  1:733988/139‑1 (MQ=255)

                                         ccTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACgg                                                                                            <  1:625307/139‑1 (MQ=255)

                                         ccTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACgg                                                                                            <  1:606188/139‑1 (MQ=255)

                                          cTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAATATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa                                                                                           <  2:851655/139‑1 (MQ=255)

                                              tCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGt                                                                                       <  2:1053041/139‑1 (MQ=255)

                                                 cAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGca                                                                                    <  2:812/139‑1 (MQ=255)

                                                     gcCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACAtt                                                                                <  2:562096/139‑1 (MQ=255)

                                                         aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc                                                                                                                                    <  2:984219/83‑1 (MQ=255)

                                                         aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc                                                                                                                                    >  1:984219/1‑83 (MQ=255)

                                                         aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt                                                                                                       <  1:586656/112‑1 (MQ=255)

                                                         aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt                                                                                                       >  2:586656/1‑112 (MQ=255)

                                                         aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATtgatg                                                                            <  2:506285/139‑1 (MQ=255)

                                                              tggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                       <  1:974746/139‑1 (MQ=255)

                                                                 cATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTCACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGt                                                                    >  1:872625/1‑139 (MQ=255)

                                                                   ttACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc                                                                                                                                    >  1:450430/1‑73 (MQ=255)

                                                                   ttACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc                                                                                                                                    <  2:450430/73‑1 (MQ=255)

                                                                          cagacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAcc                                                           >  1:1035622/1‑139 (MQ=255)

                                                                          cagacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAcc                                                           >  2:981556/1‑139 (MQ=255)

                                                                           agacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGca                                                                                    <  1:193458/113‑1 (MQ=255)

                                                                           agacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGca                                                                                    >  2:193458/1‑113 (MQ=255)

                                                                             acCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt                                                                                                       <  1:856811/92‑1 (MQ=255)

                                                                             acCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt                                                                                                       >  2:856811/1‑92 (MQ=255)

                                                                                 tttCGGACGTTGTGCTACGAGATGGGAGCGGGAATGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTAGCACCGTGCaaa                                                    >  1:727717/1‑139 (MQ=255)

                                                                                            gtgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGc                                         >  1:875988/1‑139 (MQ=255)

                                                                                             tgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTAc                                                                                              >  2:358614/1‑85 (MQ=255)

                                                                                             tgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTAc                                                                                              <  1:358614/85‑1 (MQ=255)

                                                                                               cTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGAt                                      >  1:929715/1‑139 (MQ=255)

                                                                                                    agaTGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCgaa                                 <  1:8586/139‑1 (MQ=255)

                                                                                                         ggAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAAccc                                                                                                                              <  2:1163548/41‑1 (MQ=255)

                                                                                                         ggAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAAccc                                                                                                                              >  1:1163548/1‑41 (MQ=255)

                                                                                                               ggATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAg                      >  2:1089915/1‑139 (MQ=255)

                                                                                                                      cAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagc               <  2:1066385/139‑1 (MQ=255)

                                                                                                                           tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACa                                                                                                          <  1:738812/43‑1 (MQ=255)

                                                                                                                           tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACa                                                                                                          >  2:738812/1‑43 (MQ=255)

                                                                                                                           tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAc                                                            <  2:264610/89‑1 (MQ=255)

                                                                                                                           tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAc                                                            >  1:264610/1‑89 (MQ=255)

                                                                                                                                   gatgCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTg  >  2:1072541/1‑139 (MQ=255)

TCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTG  >  NC_000913/3410271‑3410540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A