Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,410,406	T→C	S43P (TCT→CCT)	dusB →	tRNA‑dihydrouridine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,410,406	0	T	C	100.0%	118.9 / NA	34	S43P (TCT→CCT)	dusB	tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/18); total (16/18)

CTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG  >  NC_000913/3410272‑3410541

cTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc                                                                                                                                     <  2:249965/139‑1 (MQ=255)

   aaCTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTaa                                                                                                                                  >  1:6069/1‑139 (MQ=255)

      tCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAAccc                                                                                                                               <  1:580023/139‑1 (MQ=255)

          gcgcATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCcac                                                                                                                             <  2:532678/137‑1 (MQ=255)

          gcgcATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCcac                                                                                                                             >  1:532678/1‑137 (MQ=255)

                            gCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACaaa                                                                                                         >  1:165797/1‑139 (MQ=255)

                             cTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt                                                                                                        >  1:357687/1‑139 (MQ=255)

                                  aaaTCGTCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGt                                                                                                   >  1:426299/1‑139 (MQ=255)

                                       gCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACg                                                                                              >  1:17590/1‑139 (MQ=255)

                                       accTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACg                                                                                              >  2:286547/2‑139 (MQ=255)

                                         cTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa                                                                                            <  2:17590/139‑1 (MQ=255)

                                         cTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa                                                                                            <  1:305328/139‑1 (MQ=255)

                                         cTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa                                                                                            <  2:426299/139‑1 (MQ=255)

                                                cAGCGCCCATGCCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAACCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGca                                                                                     <  2:525176/139‑1 (MQ=255)

                                                        aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATtgatg                                                                             <  1:119119/139‑1 (MQ=255)

                                                                           gacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtct                                                                                                      >  1:463276/1‑95 (MQ=255)

                                                                           gacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtct                                                                                                      <  2:463276/95‑1 (MQ=255)

                                                                                    ggACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGc                                                 <  2:357687/139‑1 (MQ=255)

                                                                                          tgtgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAg                                           <  1:512947/139‑1 (MQ=255)

                                                                                          tgtgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAg                                           <  2:458435/139‑1 (MQ=255)

                                                                                                aCGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATcc                                     >  1:614211/1‑139 (MQ=255)

                                                                                                    gaTGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        >  2:744838/1‑100 (MQ=255)

                                                                                                    gaTGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:744838/100‑1 (MQ=255)

                                                                                                           gCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:298119/93‑1 (MQ=255)

                                                                                                           gCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        >  1:298119/1‑93 (MQ=255)

                                                                                                                 ttGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGacg                    <  1:780261/139‑3 (MQ=255)

                                                                                                                    aCAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcag                 <  1:286547/139‑1 (MQ=255)

                                                                                                                     cAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagc                <  2:6069/139‑1 (MQ=255)

                                                                                                                         aTCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATtgatga                                                                            >  1:585009/1‑75 (MQ=255)

                                                                                                                         aTCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATtgatga                                                                            <  2:585009/75‑1 (MQ=255)

                                                                                                                          tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAg                                           <  2:163263/107‑1 (MQ=255)

                                                                                                                          tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAg                                           >  1:163263/1‑107 (MQ=255)

                                                                                                                          tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTa           >  1:240520/1‑139 (MQ=255)

                                                                                                                              agaTGATGCCTTCCAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTaa       >  1:470680/1‑139 (MQ=255)

                                                                                                                                   atgCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTgg  >  1:258663/1‑139 (MQ=255)

CTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG  >  NC_000913/3410272‑3410541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A