Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 780 68.3 3343050 93.5% 3125751 102.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 518,721 C→A M143I (ATG→ATT)  ybbO ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913518,7210CA100.0% 156.7 / NA 49M143I (ATG→ATT) ybbOputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/24);  total (25/24)

CAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATA  >  NC_000913/518592‑518852
                                                                                                                                 |                                                                                                                                   
cAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATa                                                                                                                            >  2:1007265/1‑139 (MQ=255)
    cTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGcc                                                                                                                        <  1:742217/139‑1 (MQ=255)
             aTTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGt                                                                                                               >  1:578712/1‑139 (MQ=255)
                 cGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCgg                                                                                                           >  1:513699/1‑139 (MQ=255)
                   cTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTa                                                                                                         >  2:15814/1‑139 (MQ=255)
                         cgcAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATcg                                                                                                   <  1:15814/139‑1 (MQ=255)
                              cTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCAc                                                                                                                  >  1:1106454/1‑119 (MQ=255)
                              cTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGt                                                                                              >  1:977574/1‑139 (MQ=255)
                              cTCCATGCGCAGTGCATCTGACCACGCCTCCACCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCAc                                                                                                                  <  2:1106454/119‑1 (MQ=255)
                                 cATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAAc                                                                                           <  2:1241626/139‑1 (MQ=255)
                                  aTGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtaat                                                                                                                                   <  1:1612608/98‑1 (MQ=255)
                                  aTGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtaat                                                                                                                                   >  2:1612608/1‑98 (MQ=255)
                                    gcgcAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAgg                                                                                        <  2:513699/139‑1 (MQ=255)
                                      gcAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGgcg                                                                                      <  1:1165078/139‑1 (MQ=255)
                                        aGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACa                                                                                          >  2:1009032/1‑133 (MQ=255)
                                        aGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACa                                                                                          <  1:1009032/133‑1 (MQ=255)
                                         gTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCAt                                                                                   <  1:1295343/139‑1 (MQ=255)
                                          tGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtaat                                                                                                                                   <  2:1242251/90‑1 (MQ=255)
                                          tGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCa                                                                                    >  1:1391967/1‑137 (MQ=255)
                                          tGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCa                                                                                    <  2:1391967/137‑1 (MQ=255)
                                             aTCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTg                                                                               >  1:1639265/1‑139 (MQ=255)
                                              tCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGa                                                                              >  2:966251/1‑139 (MQ=255)
                                                      cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACTATACGCCCTTCACCGTGCGGTAACATcg                                                                                                   <  2:1034919/110‑1 (MQ=255)
                                                      cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATcg                                                                                                   >  1:1034919/1‑110 (MQ=255)
                                                      cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgtgt                                                                      >  1:148830/1‑139 (MQ=255)
                                                                      ttACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTgg                                                      <  2:1330269/139‑1 (MQ=255)
                                                                            gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTaa                                                                                            <  1:78132/95‑1 (MQ=255)
                                                                            gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTaa                                                                                            >  2:78132/1‑95 (MQ=255)
                                                                            gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGaaa                                                >  2:363913/1‑139 (MQ=255)
                                                                                cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAAt                                                                                                                             >  1:833370/1‑58 (MQ=255)
                                                                                cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAAt                                                                                                                             >  1:878959/1‑58 (MQ=255)
                                                                                cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAAt                                                                                                                             <  2:878959/58‑1 (MQ=255)
                                                                                cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAAt                                                                                                                             <  2:833370/58‑1 (MQ=255)
                                                                                cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActg                                            <  2:977574/139‑1 (MQ=255)
                                                                                                cGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCa                            <  2:148830/139‑1 (MQ=255)
                                                                                                cGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCa                            <  1:1007265/139‑1 (MQ=255)
                                                                                                 ggCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCAc                           >  2:1498219/1‑139 (MQ=255)
                                                                                                 ggCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCAc                           >  2:670648/1‑139 (MQ=255)
                                                                                                               tCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAct                                             >  1:107242/1‑107 (MQ=255)
                                                                                                               tCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAct                                             <  2:107242/107‑1 (MQ=255)
                                                                                                                cccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa            <  2:1639265/139‑1 (MQ=255)
                                                                                                                 ccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAg           <  1:670648/139‑1 (MQ=255)
                                                                                                                  cATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActgc                                           >  2:322021/1‑106 (MQ=255)
                                                                                                                  cATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActgc                                           <  1:322021/106‑1 (MQ=255)
                                                                                                                   aTCACCGATGTTGTAATCACAATACGCCCTTCACCGTGCGGTAAAATCGCGGGt                                                                                              >  1:1179688/1‑54 (MQ=38)
                                                                                                                   aTCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGt                                                                                              <  2:1179688/54‑1 (MQ=255)
                                                                                                                    tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCAt                                   >  1:114929/1‑112 (MQ=255)
                                                                                                                    tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCAt                                   <  2:114929/112‑1 (MQ=255)
                                                                                                                    tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTg                      <  1:561415/125‑1 (MQ=255)
                                                                                                                    tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTg                      >  2:561415/1‑125 (MQ=255)
                                                                                                                        cGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCa    >  2:696062/1‑139 (MQ=255)
                                                                                                                          atgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATa  >  2:1560821/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                   
CAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATA  >  NC_000913/518592‑518852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A