Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	518,721	C→A	M143I (ATG→ATT)	ybbO ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	518,721	0	C	A	100.0%	112.4 / NA	35	M143I (ATG→ATT)	ybbO	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/18); total (17/18)

AGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATAC  >  NC_000913/518593‑518853

aGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATAc                                                                                                                            <  1:744579/139‑1 (MQ=255)

      aCTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCtt                                                                                                                      >  1:714119/1‑139 (MQ=255)

            aTTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGt                                                                                                                <  1:150180/139‑1 (MQ=255)

                cGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATcaca                                                                                                                                <  2:13139/119‑1 (MQ=255)

                cGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATcaca                                                                                                                                >  1:13139/1‑119 (MQ=255)

                  cTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTa                                                                                                          <  2:441688/139‑1 (MQ=255)

                                 aTGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACa                                                                                           <  1:591965/139‑1 (MQ=255)

                                         tGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATg                                                                                   >  2:836584/1‑139 (MQ=255)

                                              cTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAg                                                                              >  2:646694/1‑139 (MQ=255)

                                                     cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgtgt                                                                       >  1:108950/1‑139 (MQ=255)

                                                                cATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGaaaaa                                                            >  1:787064/1‑139 (MQ=255)

                                                                           gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCGCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATcg                                                                                                    <  2:151065/88‑1 (MQ=255)

                                                                           gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCGCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATcg                                                                                                    >  1:151065/1‑88 (MQ=255)

                                                                             cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCg                                                                                                                 <  1:622808/73‑1 (MQ=255)

                                                                             cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCg                                                                                                                 >  2:622808/1‑73 (MQ=255)

                                                                             cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAc                                               >  1:253118/1‑139 (MQ=255)

                                                                              gcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGgc                                                                                        >  1:117316/1‑97 (MQ=255)

                                                                              gcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGgc                                                                                        <  2:117316/97‑1 (MQ=255)

                                                                              gcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAct                                              <  1:968806/139‑1 (MQ=255)

                                                                              gcgTAAGCGCCACGAACCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAct                                              <  2:758900/139‑1 (MQ=255)

                                                                               cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActg                                             >  1:187390/1‑139 (MQ=255)

                                                                               cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActg                                             >  1:305432/1‑139 (MQ=255)

                                                                               cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActg                                             >  2:42197/1‑139 (MQ=255)

                                                                                gTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActgc                                            <  2:108950/139‑1 (MQ=255)

                                                                                               cGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCa                             <  2:648023/139‑1 (MQ=255)

                                                                                                  cGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACgg                          >  1:554832/1‑139 (MQ=255)

                                                                                                    tGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCt                        <  2:187390/139‑1 (MQ=255)

                                                                                                      gagaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGa                      <  1:238559/139‑1 (MQ=255)

                                                                                                      gagaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGa                      >  1:222663/1‑139 (MQ=255)

                                                                                                       agaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGAt                     >  2:112921/1‑139 (MQ=255)

                                                                                                               cccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa             <  2:305432/139‑1 (MQ=255)

                                                                                                               cccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa             <  1:836584/139‑1 (MQ=255)

                                                                                                               cccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa             <  2:554832/139‑1 (MQ=255)

                                                                                                                    cACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAggggg        <  2:222663/139‑1 (MQ=255)

                                                                                                                          tgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATAc  >  1:938957/1‑139 (MQ=255)

AGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATAC  >  NC_000913/518593‑518853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A