Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A4 F1 I2 R1 | 780 | 68.3 | 3343050 | 93.5% | 3125751 | 102.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,533,045 | C→T | intergenic (+93/‑7) | ydcC → / → pptA | H repeat‑associated putative transposase/4‑oxalocrotonate tautomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,533,045 | 0 | C | T | 100.0% | 241.1 / NA | 75 | intergenic (+93/‑7) | ydcC/pptA | H repeat‑associated putative transposase/4‑oxalocrotonate tautomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (36/39); total (36/39) | |||||||||||
AAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCT > NC_000913/1532910‑1533180 | aaaCTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTa > 1:1424617/1‑139 (MQ=255) cTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATc < 2:530975/139‑1 (MQ=255) tACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTGCCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCa > 2:1529482/1‑139 (MQ=255) cTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGc < 1:813450/139‑1 (MQ=255) gTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATAATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCg > 2:1092373/1‑139 (MQ=255) gAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGt > 2:1415219/1‑139 (MQ=255) cGGGCTTTCGTAATCTTGCGCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGtttt < 1:1092373/139‑1 (MQ=255) cTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCcgc < 1:415016/139‑1 (MQ=255) ttGCCATGGGCATTATGGTCGTATAAACGCTTTACCGAAACAATGACATTCACAAGTTGTTTTAATTTTACTACCATCTTCAAAAAGGAGTGTATTCTTGCCGCCCATCAAAATT‑AATGTTTTCCGCGTGAACTGGACg < 2:959364/139‑1 (MQ=255) gCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTaa > 2:1109636/1‑93 (MQ=255) gCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTaa < 1:1109636/93‑1 (MQ=255) gCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGAc > 1:1001905/1‑137 (MQ=255) gCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGAc < 2:1001905/137‑1 (MQ=255) gCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa > 2:814800/1‑139 (MQ=255) gCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa > 1:385349/1‑139 (MQ=255) cccTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGaa > 1:1097870/1‑139 (MQ=255) tattGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAGAAGGAGTGTAATCATGCCGCACATCGACATTa < 2:482281/103‑1 (MQ=255) tattGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAGAAGGAGTGTAATCATGCCGCACATCGACATTa > 1:482281/1‑103 (MQ=255) tattGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAag > 1:1441023/1‑139 (MQ=255) cGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCAc < 2:587996/139‑1 (MQ=255) tttACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGc > 2:1004681/1‑71 (MQ=255) tttACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGc < 1:1004681/71‑1 (MQ=255) ttACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctg > 2:1214428/1‑133 (MQ=255) ttACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctg < 1:1214428/133‑1 (MQ=255) ttACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGata > 1:1152070/1‑139 (MQ=255) tACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCg < 2:818205/71‑1 (MQ=255) tACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCg > 1:818205/1‑71 (MQ=255) tACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGatat > 2:1386451/1‑139 (MQ=255) aCCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATAtt > 2:1504552/1‑139 (MQ=255) cGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTAc < 2:1097870/139‑1 (MQ=255) acaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGaa < 1:1411726/102‑1 (MQ=255) acaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGaa > 2:1411726/1‑102 (MQ=255) aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCg > 1:1592051/1‑61 (MQ=255) aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCg < 2:1592051/61‑1 (MQ=255) aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGttt < 1:279107/81‑1 (MQ=255) aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGttt > 2:279107/1‑81 (MQ=255) aCATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgct > 1:632077/1‑118 (MQ=255) aCATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgct < 2:632077/118‑1 (MQ=255) tCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTc < 1:1529482/139‑1 (MQ=255) tgtgtgTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTc > 2:426378/1‑133 (MQ=255) tgtgtgTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTc < 1:426378/133‑1 (MQ=255) tgtgtgTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATc > 2:494725/1‑139 (MQ=255) gtgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCt < 2:1152070/139‑1 (MQ=255) gtgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCt > 1:1375968/1‑139 (MQ=255) gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa < 2:1375968/139‑1 (MQ=255) gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa < 1:494725/139‑1 (MQ=255) gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa < 2:1424617/139‑1 (MQ=255) tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGaa > 2:350787/1‑139 (MQ=255) gtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACt < 1:232236/99‑1 (MQ=255) gtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAAc < 2:385349/139‑1 (MQ=255) gtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAAATGGACGAACAACAAAAAGCAGCACt > 2:232236/1‑99 (MQ=255) ttAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCAt < 2:920741/132‑1 (MQ=255) ttAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCAt > 1:920741/1‑132 (MQ=255) ttAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACa > 2:1456375/1‑139 (MQ=255) ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCg > 2:1235922/1‑36 (MQ=255) ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCg < 1:1235922/36‑1 (MQ=255) ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACt > 2:1422583/1‑92 (MQ=255) ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACt < 1:1422583/92‑1 (MQ=255) tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGa < 1:1386451/139‑1 (MQ=255) aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACAt < 1:30916/45‑1 (MQ=255) aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACAt > 2:30916/1‑45 (MQ=255) aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGAc < 2:1441023/139‑1 (MQ=255) tGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa > 1:858652/1‑126 (MQ=255) tGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa < 2:858652/126‑1 (MQ=255) cTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGAt < 1:350787/139‑1 (MQ=255) tttCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATa > 2:419321/1‑139 (MQ=255) taaaaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACg < 1:838325/97‑1 (MQ=255) tCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGATGCAGATATTACCGACg > 2:838325/1‑99 (MQ=255) aaaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctgc > 2:1471975/1‑83 (MQ=255) aaaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctgc < 1:1471975/83‑1 (MQ=255) aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctg > 1:244425/1‑81 (MQ=255) aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctg < 2:244425/81‑1 (MQ=255) aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAt < 1:1456375/139‑1 (MQ=255) aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAt < 1:1504552/139‑1 (MQ=255) gAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGct < 1:814800/139‑1 (MQ=255) | AAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCT > NC_000913/1532910‑1533180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |