Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,533,045 |
C→T |
intergenic (+93/‑7) |
ydcC → / → pptA |
H repeat‑associated putative transposase/4‑oxalocrotonate tautomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,533,045 | 0 | C | T | 100.0%
| 36.3
/ NA
| 14 | intergenic (+93/‑7) | ydcC/pptA | H repeat‑associated putative transposase/4‑oxalocrotonate tautomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/6); total (8/6) |
AAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGAC > NC_000913/1532910‑1533162
|
aaaCTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTa > 2:281903/1‑139 (MQ=255)
tGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAACAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACg < 1:158767/139‑1 (MQ=255)
gCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa > 1:503292/1‑139 (MQ=255)
gCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa > 2:9780/1‑139 (MQ=255)
tCGTATAAACGCTTTACCGGACACATGACATTCACATTGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCAGCACATCGACATTAAATGTTTTCCGCGTGAATTGGACGAACAACAAAAAgcagca > 2:778564/1‑139 (MQ=255)
gCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACAt > 1:108371/1‑80 (MQ=255)
gCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACAt < 2:108371/80‑1 (MQ=255)
acacATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGa < 1:281903/139‑1 (MQ=255)
gtgtTAACTTTACTGACATCTTTCAAAAGGAGTATAATCATGCCGCACATAGACATTATATGTTTACCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa < 1:778564/139‑1 (MQ=255)
tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGaa > 1:388069/1‑139 (MQ=255)
tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGa < 2:503292/139‑1 (MQ=255)
aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGa < 1:155892/99‑1 (MQ=255)
aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGa > 2:155892/1‑99 (MQ=255)
aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGAc > 2:80662/1‑139 (MQ=255)
|
AAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGAC > NC_000913/1532910‑1533162
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
